Abstract: A method for mass-producing plants includes a step of accommodating a liquid cultivation medium and a plant material into each of a plurality of culture bags that are configured so as to be capable of standing up without assistance by being opened up from a folded state, sealing openings of the culture bags, and arranging the sealed culture bags in a row in a predetermined cultivation space, and a step of cultivating the plant material within each of the plurality of culture bags with an interior of each of the plurality of culture bags arranged in a row in the cultivation space being maintained at an environment appropriate for cultivation of the plant material.

Abstract: The present invention provides a method for transforming a plant of the family Araceae, comprising introducing a foreign gene into an embryogenic callus of the plant through infection of the callus with Agrobacterium or a microorganism capable of introducing a gene therein by an analogous mechanism to that of Agrobacterium, thereby preparing a transformed embryogenic callus.

Abstract: A novel method is provided, which makes it possible to perform mass propagation of a plant of the family Araceae, the family Bromeliaceae, or the family Marantaceae. Specifically, a method for producing an embryogenic callus, comprising culturing a leaf sheath or a part thereof of a plant selected from the group consisting of the family Araceae, the family Bromeliaceae, and the family Marantaceae as an explant and then inducing an embryogenic callus and a propagation method comprising regenerating a plant through induction of a somatic embryo from the embryogenic callus obtained by the above method are also provided herein.

Abstract: The present invention relates to improved promoters and utilization thereof, in particular to promoters which are improved so as not to undergo methylation in the course of constructing transformants, and utilization thereof. According to the present invention, the expression efficiency of a structural gene can be enhanced even in a plant, e.g. chrysanthemum, which has weak expression of the structural gene by a cauliflower mosaic virus 35S promoter which as been considered a high expression promoter for plants.

Abstract: The present invention relates to improved promoters and utilization thereof, in particular to promoters which are improved so as not to undergo methylation in the course of constructing transformants, and utilization thereof. The improved promoters of the present invention are represented by the following (1) and (2): (1) a DNA comprising the nucleotide sequences shown in SEQ ID NOS: 1 to 4; (2) a DNA comprising a nucleotide sequence consisting of the nucleotide sequences shown in SEQ ID NOS 1 to 4, wherein one to several nucleotides are deleted, added or inserted and the deleted, added or inserted sequence is free from any consecutive sequences represented by CG, CAG, CTG, CCG or CGG; the DNA having promoter activity. According to the present invention, the expression efficiency of a structural gene can be enhanced even in a plant, e.g. chrysanthemum, which has weak expression of the structural gene by a cauliflower mosaic virus 35S promoter which has been considered a high expression promoter for plants.

Abstract: The present invention relates to improved promoters and utilization thereof, in particular to promoters which are improved so as not to undergo methylation in the course of constructing transformants, and utilization thereof. According to the present invention, the expression efficiency of a structural gene can be enhanced even in a plant, e.g. chrysanthemum, which has weak expression of the structural gene by a cauliflower mosaic virus 35S promoter which as been considered a high expression promoter for plants.

Abstract: Provided is a plant having improved efficiency in propagation by cutting resulting from the enhanced rooting efficiency, and improved vase life. A method of producing a transformed plant having improved rooting efficiency and/or prolonged vase life, comprising transforming a plant using a gene wherein a DNA encoding a protein that binds to a stress-responsive element contained in a stress-responsive promoter and regulates the transcription of a gene located downstream of the element is ligated downstream of the stress-responsive promoter.

Abstract: The present invention relates to improved promoters and utilization thereof, in particular to promoters which are improved so as not to undergo methylation in the course of constructing transformants, and utilization thereof.

Abstract: This invention provides a process for easy and large-scale production of tubers from plants belonging to the genus Solanum, wherein the improvement resides in that tubers are formed with high efficiency by exposing stolons and shoots, elongated in a liquid phase under a carbon dioxide-applied condition during light periods in a shoot propagation step, to a gaseous phase in a subsequent tuber formation step, and that tubers are formed in a large number not only in a boundary medium surface area but also in a gaseous phase area distant from the medium surface by culturing the stolons and shoots during the tuber formation step in a culture jar with aeration of the gaseous phase. By employing such culture steps and conditions, tuber forming efficiency and quality of the produced tubers are improved.