Abstract: A method of using a sequencing cell includes applying an alternating signal across a nanopore of the sequencing cell. The method further includes acquiring a first set of voltage data during a first portion of a plurality of cycles of the alternating signal. The method further includes determining a shifted set of voltage data from the first set of voltage data, computing difference data values by computing differences between data points of the first set of voltage data and corresponding data points of the shifted set of voltage data, identifying a plurality of noise data points as data points having difference data values that are larger than a first threshold value, and removing the plurality of noise data points from the first set of voltage data.

Abstract: A method of using a sequencing cell includes applying an alternating signal across a nanopore of the sequencing cell. The method further includes acquiring a first set of voltage data during a first portion of a plurality of cycles of the alternating signal. The method further includes determining a shifted set of voltage data from the first set of voltage data, computing difference data values by computing differences between data points of the first set of voltage data and corresponding data points of the shifted set of voltage data, identifying a plurality of noise data points as data points having difference data values that are larger than a first threshold value, and removing the plurality of noise data points from the first set of voltage data.

Abstract: An alternating signal is applied across a nanopore of a sequencing cell, the nanopore being configured to receive a tag that is connected to a nucleotide, thereby creating a threading event. A first set of voltage data is acquired during a first portion of a plurality of cycles of the alternating signal. Each data point of the first set of voltage data corresponds to a value of a resistance of the nanopore at a different time, where the resistance of the nanopore changes when the tag is received within the nanopore. A shifted set of voltage data is determined from the first set of voltage data and difference data is computed by computing differences between data points of the first set of voltage data and corresponding data points of the shifted set of voltage data. Threading events may be identified based on the difference data.

Abstract: An alternating signal is applied across a nanopore of a sequencing cell, the nanopore being configured to receive a tag that is connected to a nucleotide, thereby creating a threading event. A first set of voltage data is acquired during a first portion of a plurality of cycles of the alternating signal. Each data point of the first set of voltage data corresponds to a value of a resistance of the nanopore at a different time, where the resistance of the nanopore changes when the tag is received within the nanopore. A shifted set of voltage data is determined from the first set of voltage data and difference data is computed by computing differences between data points of the first set of voltage data and corresponding data points of the shifted set of voltage data. Threading events may be identified based on the difference data.

Abstract: Provided are methods, compositions, and systems for the detection of a target analyte. Also provided are methods, compositions, and systems for determining the concentration one or more target analytes in fluid solution. The compositions include nanopore conjugates in which a nanopore protein monomer is joined to a capture tag. Tethered to the nanopore protein conjugate is an analyte ligand directed to a specific analyte. When a voltage is applied across a nanopore assembly including the nanopore conjugate, the nanopore captures the capture tag at a given capture rate. In the presence of the analyte to the analyte ligand, however, the capture rate of the capture tag changes, thus permitting detection of the analyte by the nanopore assembly. Further, based on the capture rate associated with binding between the analyte and the analyte ligand, the concentration of the analyte can be determined using association/dissociation kinetics.

Abstract: A method of using a sequencing cell includes applying an alternating signal across a nanopore of the sequencing cell. The method further includes acquiring a first set of voltage data during a first portion of a plurality of cycles of the alternating signal. The method further includes determining a shifted set of voltage data from the first set of voltage data, computing difference data values by computing differences between data points of the first set of voltage data and corresponding data points of the shifted set of voltage data, identifying a plurality of noise data points as data points having difference data values that are larger than a first threshold value, and removing the plurality of noise data points from the first set of voltage data.

Abstract: An alternating signal is applied across a nanopore of a sequencing cell, the nanopore being configured to receive a tag that is connected to a nucleotide, thereby creating a threading event. A first set of voltage data is acquired during a first portion of a plurality of cycles of the alternating signal. Each data point of the first set of voltage data corresponds to a value of a resistance of the nanopore at a different time, where the resistance of the nanopore changes when the tag is received within the nanopore. A shifted set of voltage data is determined from the first set of voltage data and difference data is computed by computing differences between data points of the first set of voltage data and corresponding data points of the shifted set of voltage data. Threading events may be identified based on the difference data.