Patents by Inventor Karel Svoboda
Karel Svoboda has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11572401Abstract: Genetically encoded calcium indicator (GECI) polypeptides and the nucleic acid molecules encoding such polypeptides are provided. In addition, methods of using such nucleic acids and polypeptides in methods of screening for agonists or antagonists of G-protein coupled receptor (GPCR) or ion channels and methods of monitoring neural activity also are provided.Type: GrantFiled: November 21, 2018Date of Patent: February 7, 2023Assignee: Howard Hughes Medical InstituteInventors: Douglas Kim, Karel Svoboda, Loren Looger, Eric Schreiter
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Patent number: 10901194Abstract: A microscope system including: a source of light; a sample objective configured for focusing the light at a focal plane within a sample; a remote focus unit configured for changing a position of the focal plane along an axis perpendicular to the focal plane; one or more optical element configured for directing the focused light to a location within the focal plane; and a detector configured for detecting light emitted from the focal plane within the sample; wherein the one or more optical element is located after the remote focus unit along a beam path of the light from the source to the sample objective, such that the changing the position of the focal plane along the axis is performed before the directing the focused light to the location within the focal plane.Type: GrantFiled: November 15, 2018Date of Patent: January 26, 2021Assignee: Howard Hughes Medical InstituteInventors: Karel Svoboda, Daniel Flickinger, Nicholas Sofroniew
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Patent number: 10509026Abstract: Genetically encoded calcium indicator (GECI) polypeptides and the nucleic acid molecules encoding such polypeptides are provided. In addition, methods of using such nucleic acids and polypeptides in methods of screening for agonists or antagonists of G-protein coupled receptor (GPCR) or ion channels and methods of monitoring neural activity also are provided.Type: GrantFiled: April 12, 2018Date of Patent: December 17, 2019Assignee: Howard Hughes Medical InstituteInventors: Loren Looger, Karel Svoboda, Douglas Kim, Rex Kerr
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Publication number: 20190153067Abstract: Genetically encoded calcium indicator (GECI) polypeptides and the nucleic acid molecules encoding such polypeptides are provided. In addition, methods of using such nucleic acids and polypeptides in methods of screening for agonists or antagonists of G-protein coupled receptor (GPCR) or ion channels and methods of monitoring neural activity also are provided.Type: ApplicationFiled: November 21, 2018Publication date: May 23, 2019Inventors: Douglas Kim, Karel Svoboda, Loren Looger, Eric Schreiter
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Patent number: 10295811Abstract: A microscope system including: a source of light; a sample objective configured for focusing the light at a focal plane within a sample; a remote focus unit configured for changing a position of the focal plane along an axis perpendicular to the focal plane; one or more optical element configured for directing the focused light to a location within the focal plane; and a detector configured for detecting light emitted from the focal plane within the sample; wherein the one or more optical element is located after the remote focus unit along a beam path of the light from the source to the sample objective, such that the changing the position of the focal plane along the axis is performed before the directing the focused light to the location within the focal plane.Type: GrantFiled: November 1, 2016Date of Patent: May 21, 2019Assignee: Howard Hughes Medical InstituteInventors: Karel Svoboda, Daniel Flickinger, Nicholas Sofroniew
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Publication number: 20190101735Abstract: A microscope system including: a source of light; a sample objective configured for focusing the light at a focal plane within a sample; a remote focus unit configured for changing a position of the focal plane along an axis perpendicular to the focal plane; one or more optical element configured for directing the focused light to a location within the focal plane; and a detector configured for detecting light emitted from the focal plane within the sample; wherein the one or more optical element is located after the remote focus unit along a beam path of the light from the source to the sample objective, such that the changing the position of the focal plane along the axis is performed before the directing the focused light to the location within the focal plane.Type: ApplicationFiled: November 15, 2018Publication date: April 4, 2019Inventors: Karel Svoboda, Daniel Flickinger, Nicholas Sofroniew
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Publication number: 20180299429Abstract: Genetically encoded calcium indicator (GECI) polypeptides and the nucleic acid molecules encoding such polypeptides are provided. In addition, methods of using such nucleic acids and polypeptides in methods of screening for agonists or antagonists of G-protein coupled receptor (GPCR) or ion channels and methods of monitoring neural activity also are provided.Type: ApplicationFiled: April 12, 2018Publication date: October 18, 2018Inventors: Loren Looger, Karel Svoboda, Douglas Kim, Rex Kerr
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Patent number: 10053492Abstract: Protein indicators useful for calcium imaging, in particular, red genetically-encoded calcium indicators (GECIs) disclosed herein rival best-of-class green GECIs in terms of sensitivity for detecting neural activity, and can be monitored in vivo. The presently-disclosed subject matter further includes a method of monitoring cell activity comprising stimulating a cell comprising a red GECI polypeptide; and detecting fluorescence emitted by the cell.Type: GrantFiled: March 17, 2017Date of Patent: August 21, 2018Assignee: HOWARD HUGHES MEDICAL INSTITUTEInventors: Douglas S. Kim, Loren L. Looger, Eric R. Schreiter, Karel Svoboda
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Patent number: 9945844Abstract: Genetically encoded calcium indicator (GECI) polypeptides and the nucleic acid molecules encoding such polypeptides are provided. In addition, methods of using such nucleic acids and polypeptides in methods of screening for agonists or antagonists of G-protein coupled receptor (GPCR) or ion channels and methods of monitoring neural activity also are provided.Type: GrantFiled: November 3, 2016Date of Patent: April 17, 2018Assignee: Howard Hughes Medical InstituteInventors: Loren Looger, Karel Svoboda, Douglas Kim, Rex Kerr
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Publication number: 20170292943Abstract: Genetically encoded calcium indicator (GECI) polypeptides and the nucleic acid molecules encoding such polypeptides are provided. In addition, methods of using such nucleic acids and polypeptides in methods of screening for agonists or antagonists of G-protein coupled receptor (GPCR) or ion channels and methods of monitoring neural activity also are provided.Type: ApplicationFiled: November 3, 2016Publication date: October 12, 2017Inventors: Loren Looger, Karel Svoboda, Douglas Kim, Rex Kerr
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Publication number: 20170198015Abstract: Protein indicators useful for calcium imaging, in particular, red genetically-encoded calcium indicators (GECIs) disclosed herein rival best-of-class green GECIs in terms of sensitivity for detecting neural activity, and can be monitored in vivo. The presently-disclosed subject matter further includes a method of monitoring cell activity comprising stimulating a cell comprising a red GECI polypeptide; and detecting fluorescence emitted by the cell.Type: ApplicationFiled: March 17, 2017Publication date: July 13, 2017Inventors: Douglas S. KIM, Loren L. LOOGER, Eric R. SCHREITER, Karel SVOBODA
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Patent number: 9644007Abstract: Protein indicators useful for calcium imaging, in particular, red genetically-encoded calcium indicators (GECIs) disclosed herein rival best-of-class green GECIs in terms of sensitivity for detecting neural activity, and can be monitored in vivo. The presently-disclosed subject matter further includes a method of monitoring cell activity comprising stimulating a cell comprising a red GECI polypeptide; and detecting fluorescence emitted by the cell.Type: GrantFiled: December 18, 2015Date of Patent: May 9, 2017Assignee: Howard Hughes Medical InstituteInventors: Douglas S. Kim, Loren L. Looger, Eric R. Schreiter, Karel Svoboda
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Publication number: 20170123196Abstract: A microscope system including: a source of light; a sample objective configured for focusing the light at a focal plane within a sample; a remote focus unit configured for changing a position of the focal plane along an axis perpendicular to the focal plane; one or more optical element configured for directing the focused light to a location within the focal plane; and a detector configured for detecting light emitted from the focal plane within the sample; wherein the one or more optical element is located after the remote focus unit along a beam path of the light from the source to the sample objective, such that the changing the position of the focal plane along the axis is performed before the directing the focused light to the location within the focal plane.Type: ApplicationFiled: November 1, 2016Publication date: May 4, 2017Inventors: Karel Svoboda, Daniel Flickinger, Nicholas Sofroniew
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Patent number: 9518980Abstract: Genetically encoded calcium indicator (GECI) polypeptides and the nucleic acid molecules encoding such polypeptides are provided. In addition, methods of using such nucleic acids and polypeptides in methods of screening for agonists or antagonists of G-protein coupled receptor (GPCR) or ion channels and methods of monitoring neural activity also are provided.Type: GrantFiled: March 13, 2013Date of Patent: December 13, 2016Assignee: Howard Hughes Medical InstituteInventors: Loren Looger, Karel Svoboda, Douglas Kim, Rex Kerr
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Patent number: 9488642Abstract: Genetically encoded calcium indicator (GECI) polypeptides and the nucleic acid molecules encoding such polypeptides are provided. In addition, methods of using such nucleic acids and polypeptides in methods of screening for agonists or antagonists of G-protein coupled receptor (GPCR) or ion channels and methods of monitoring neural activity also are provided.Type: GrantFiled: July 16, 2015Date of Patent: November 8, 2016Assignee: Howard Hughes Medical InstituteInventors: Loren Looger, Karel Svoboda, Douglas Kim, Rex Kerr
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Publication number: 20150315258Abstract: Genetically encoded calcium indicator (GECI) polypeptides and the nucleic acid molecules encoding such polypeptides are provided. In addition, methods of using such nucleic acids and polypeptides in methods of screening for agonists or antagonists of G-protein coupled receptor (GPCR) or ion channels and methods of monitoring neural activity also are provided.Type: ApplicationFiled: July 16, 2015Publication date: November 5, 2015Inventors: Loren Looger, Karel Svoboda, Douglas Kim, Rex Kerr
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Publication number: 20140101785Abstract: Genetically encoded calcium indicator (GECI) polypeptides and the nucleic acid molecules encoding such polypeptides are provided. In addition, methods of using such nucleic acids and polypeptides in methods of screening for agonists or antagonists of G-protein coupled receptor (GPCR) or ion channels and methods of monitoring neural activity also are provided.Type: ApplicationFiled: March 13, 2013Publication date: April 10, 2014Applicant: HOWARD HUGHES MEDICAL INSTITUTEInventors: Loren Looger, Karel Svoboda, Douglas Kim, Rex Kerr
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Publication number: 20050288247Abstract: The invention provides molecular systems for inducible and reversible inactivation of synaptic transmission. These systems can be used for studying neuronal networks and for treating conditions involving abnormally high neuronal activity or excitotoxic damage.Type: ApplicationFiled: June 24, 2005Publication date: December 29, 2005Inventors: Karel Svoboda, Alla Karpova
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Publication number: 20020004632Abstract: A system and method provides automated detailed analysis of microscopic neuronal cell morphology. Accordingly, the effects of various substances on the structure and function of neurons can be evaluated based on morphology of the neurons. An algorithm is presented which utilizes a geometric approach for automatically detecting and quantifying the three-dimensional structure of dendritic spines from stacks of image data acquired using microscopy. Results are presented on the measurement of dendritic spine length, volume, density, and shape classification for both static and time-lapse images of dendrites. The approaches presented here are generalizable to other aspects of neuronal morphology.Type: ApplicationFiled: April 10, 2001Publication date: January 10, 2002Inventors: W. Brent Lindquist, Ying Ying Koh, Karel Svoboda
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Patent number: 4779757Abstract: A container for chemically aggressive liquids and gases has an internal lining which has a coating of meshed wire or expanded metal welded to at least a portion of the inner face of the container and a layer of synthetic resin putty applied to said coating.Type: GrantFiled: August 6, 1987Date of Patent: October 25, 1988Assignee: Krupp-Koppers GmbHInventors: Karl O. Fuckert, Karel Svoboda