Abstract: A computer-implemented method performed by a depository computing system includes receiving a check deposit and a withdrawal request associated with a customer account of a customer where the customer account is associated with a first amount and the withdrawal request comprises a second amount greater than the first amount, forwarding an inquiry message regarding the check deposit to a paying computing system, determining real time account status information of a payor account of a payor, and authorizing in real time relative to when the real time account status information is accessed, the withdrawal request for the second amount where the authorization occurs prior to settlement of the check deposit.

Abstract: A computer-implemented method includes receiving a check deposit and a withdrawal request associated with an account of a customer where the account is associated with a first amount and where the withdrawal request comprises a second amount greater than the first amount, forwarding an inquiry message regarding the check deposit to a paying computing system where the inquiry message includes an identifier of the paying computing system that maintains an account of a payor in a paying database, determining real time account status information of the account of the payor where the real time account status information is based on accessing an organized data structure, and authorizing the withdrawal request for the second amount in real time relative to when the real time account status information is accessed where the authorization occurs prior to settlement of the check deposit.

Abstract: A process includes receiving a check deposit and a withdrawal request associated with an account of a customer, the account associated with a first amount, the check deposit includes a second amount greater than the first amount, and the withdrawal request includes a third amount greater than the first amount. The process further includes forwarding, via an account verification system, an inquiry message regarding the check deposit to a paying computing system, the inquiry message including an identifier of the paying computing system, the inquiry message forwarded prior to settlement of the check deposit. The process further includes determining real time account status information of the account of the payor, and authorizing in real time relative to when the real time account status is accessed.

Abstract: A computer-implemented system and method receive information regarding a funds transfer from a payor to a payee. The information is received by a depository computer system. The depository computer system forwards the information regarding the funds transfer to an account verification service computer system. The depository receives an indication of a real time account status of an account of the payor. The account status information is received from a paying bank computer system that maintains the account of the payor via the account verification service computer system. Release of the funds to the payee is authorized based on the account status information received via the account verification service computer system. The authorization occurs in real time relative to when the funds transfer information is initially received.

Abstract: A computer-implemented system and method receive information regarding a funds transfer from a payor to a payee. The information is received by a depository computer system. The depository computer system forwards the information regarding the funds transfer to an account verification service computer system. The depository receives an indication of a real time account status of an account of the payor. The account status information is received from a paying bank computer system that maintains the account of the payor via the account verification service computer system. Release of the funds to the payee is authorized based on the account status information received via the account verification service computer system. The authorization occurs in real time relative to when the funds transfer information is initially received.

Abstract: A computer-implemented system and method receive information regarding a funds transfer from a payor to a payee. The information is received by a depository computer system. The depository computer system forwards the information regarding the funds transfer to an account verification service computer system. The depository receives an indication of a real time account status of an account of the payor. The account status information is received from a paying bank computer system that maintains the account of the payor via the account verification service computer system. Release of the funds to the payee is authorized based on the account status information received via the account verification service computer system. The authorization occurs in real time relative to when the funds transfer information is initially received.

Abstract: The present invention provides a method of isolating nucleic acid and protein from the same biological sample, comprising, in the following order: a) disrupting the biological sample; b) contacting the disrupted biological sample of (a) with a protein lysis buffer that lacks any component that denatures or reduces protein to produce a first lysate; c) centrifuging the first lysate of (b) to produce a first supernatant containing protein and a pellet containing nucleic acid; d) removing the first supernatant of (c), thereby isolating protein from the biological sample; e) contacting the pellet of (d) with nucleic acid lysis buffer to produce a second lysate; f) centrifuging the second lysate of (e) to produce a second supernatant containing nucleic acid; and g) removing the second supernatant of (f), thereby isolating nucleic acid from the same biological sample.

Abstract: Method and kit for providing a training course for operating a towing device.

Abstract: A computer system may manage orders for goods from a plurality of purchasers. The computer system may receive the orders, calculate an approximate volume that the goods which are the subject of each order would collectively occupy during shipment, and compare each calculated approximate volume to a pre-determine maximum allowed volume that the purchaser of the order is permitted. The computer system may accept each order whose approximate volume does not exceed the maximum allowed volume for the purchaser of the order, or reject each order whose approximate volume exceeds this maximum allowed volume.

Abstract: A computer system may manage orders for goods from a plurality of purchasers. The computer system may receive the orders, calculate an approximate volume that the goods which are the subject of each order would collectively occupy during shipment, and compare each calculated approximate volume to a pre-determine maximum allowed volume that the purchaser of the order is permitted. The computer system may accept each order whose approximate volume does not exceed the maximum allowed volume for the purchaser of the order, or reject each order whose approximate volume exceeds this maximum allowed volume.

Abstract: Systems and methods for applying a coating to an interior surface of a conduit. In one embodiment, a spray gun configured to apply a coating is attached to an extension arm which may be inserted into the bore of a pipe. The spray gun may be a thermal spray gun adapted to apply a powder coating. An evacuation system may be used to provide a volume area of reduced air pressure for drawing overspray out of the pipe interior during coating. The extension arm as well as the spray gun may be cooled to maintain a consistent temperature in the system, allowing for more consistent coating.

Abstract: Systems and methods for applying a coating to an interior surface of a conduit. In one embodiment, a spray gun configured to apply a coating is attached to an extension arm which may be inserted into the bore of a pipe. The spray gun may be a thermal spray gun adapted to apply a powder coating. An evacuation system may be used to provide a volume area of reduced air pressure for drawing overspray out of the pipe interior during coating. The extension arm as well as the spray gun may be cooled to maintain a consistent temperature in the system, allowing for more consistent coating.

Abstract: A method and system for detecting, locating and quantifying a physical phenomena such as strain or a deformation in a structure. A plurality of laterally adjacent conductors may each include a plurality of segments. Each segment is constructed to exhibit a unit value representative of a defined energy transmission characteristic. A plurality of identity groups are defined with each identity group comprising a plurality of segments including at least one segment from each of the plurality of conductors. The segments contained within an identity group are configured and arranged such that each of their associated unit values may be represented by a concatenated digit string which is a unique number relative to the other identity groups. Additionally, the unit values of the segments within an identity group maintain unique ratios with respect to the other unit values in the identity group.

Abstract: A method and system for detecting, locating and quantifying a physical phenomena such as strain or a deformation in a structure. A plurality of laterally adjacent conductors may each include a plurality of segments. Each segment is constructed to exhibit a unit value representative of a defined energy transmission characteristic. A plurality of identity groups are defined with each identity group comprising a plurality of segments including at least one segment from each of the plurality of conductors. The segments contained within an identity group are configured and arranged such that each of their associated unit values may be represented by a concatenated digit string which is a unique number relative to the other identity groups. Additionally, the unit values of the segments within an identity group maintain unique ratios with respect to the other unit values in the identity group.

Abstract: Systems and methods for applying a coating to an interior surface of a conduit. In one embodiment, a spray gun configured to apply a coating is attached to an extension arm which may be inserted into the bore of a pipe. The spray gun may be a thermal spray gun adapted to apply a powder coating. An evacuation system may be used to provide a volume area of reduced air pressure for drawing overspray out of the pipe interior during coating. The extension arm as well as the spray gun may be cooled to maintain a consistent temperature in the system, allowing for more consistent coating.

Abstract: A method and system for detecting, locating and quantifying a physical phenomena such as strain or a deformation in a structure. A plurality of laterally adjacent conductors may each include a plurality of segments. Each segment is constructed to exhibit a unit value representative of a defined energy transmission characteristic. A plurality of identity groups are defined with each identity group comprising a plurality of segments including at least one segment from each of the plurality of conductors. The segments contained within an identity group are configured and arranged such that each of their associated unit values may be represented by a concatenated digit string which is a unique number relative to the other identity groups. Additionally, the unit values of the segments within an identity group maintain unique ratios with respect to the other unit values in the identity group.

Abstract: A method and system for detecting, locating and quantifying a physical phenomena such as strain or a deformation in a structure. A plurality of laterally adjacent conductors may each include a plurality of segments. Each segment is constructed to exhibit a unit value representative of a defined energy transmission characteristic. A plurality of identity groups are defined with each identity group comprising a plurality of segments including at least one segment from each of the plurality of conductors. The segments contained within an identity group are configured and arranged such that each of their associated unit values may be represented by a concatenated digit string which is a unique number relative to the other identity groups. Additionally, the unit values of the segments within an identity group maintain unique ratios with respect to the other unit values in the identity group.

Abstract: A method and system for detecting, locating and quantifying a physical phenomena such as strain or a deformation in a structure. A plurality of laterally adjacent conductors may each include a plurality of segments. Each segment is constructed to exhibit a unit value representative of a defined energy transmission characteristic. A plurality of identity groups are defined with each identity group comprising a plurality of segments including at least one segment from each of the plurality of conductors. The segments contained within an identity group are configured and arranged such that each of their associated unit values may be represented by a concatenated digit string which is a unique number relative to the other identity groups. Additionally, the unit values of the segments within an identity group maintain unique ratios with respect to the other unit values in the identity group.

Abstract: The present invention relates to the discovery, identification and characterization of nucleic acids that encode a novel G protein coupled receptor (I5E) protein. The invention encompasses I5E nucleotides, host cell expression systems, I5E proteins, fusion proteins, polypeptides and peptides, antibodies to the receptor., transgenic animals that express an I5E transgene, or recombinant knock-out animals that do not express the I5E, antagonists and agonists of the receptor, and other compounds that modulate I5E gene expression or I5E activity that can be used for diagnosis, drug screening, clinical trial monitoring, and/or used to treat disorders such as inflammatory, central nervous system or gastrointestinal disorders.

Abstract: The present invention relates to mammalian mahogany genes, including the human mahogany gene, which are novel genes involved in the control of mammalian body weight. The invention encompasses nucleotide sequences of the mahogany gene, host cell expression systems of the mahogany gene, and hosts which have been transformed by these expression systems, including transgenic animals. The invention also encompasses novel mahogany gene products, including mahogany proteins, polypeptides and peptides containing amino acid sequences mahogany proteins, fusion proteins of mahogany proteins polypeptides and peptides, and antibodies directed against such mahogany gene products. The present invention also relates to methods and compositions for the diagnosis and treatment of mammalian body weight disorders, including obesity, cachexia, and anorexia, and for the identification of subjects susceptible to such disorders.