Abstract: This invention describes a novel method of producing bioactive recombinant proteins from plants. General methods of designing and engineering plants for expression and production of such proteins are also disclosed. Methods for the expression of Transforming Growth Factor-? (TGF-?) proteins, such as Müllerian Inhibiting Substance (MIS), in plants, and methods of producing recombinant proteins from plants are specifically disclosed. Furthermore, the present invention provides methodology for the direct expression and production of a bioactive C-terminal fragment of a TGF-? protein, such as C-terminal MIS. The new method is more cost-effective than other large-scale expression systems, by eliminating the need for costly cell culture and fermentation manufacturing facilities.

Abstract: The invention relates to the production of biologically active recombinant human and animal urokinases involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal urokinases in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting biological activity. The invention is demonstrated by working examples in which transgenic tobacco plants having recombinant expression constructs comprising human urokinase and urokinase nucleotide sequences produced biologically active urokinase. The recombinant urokinases produced in accordance with the invention may be used for a variety of purposes, including but not limited to thrombolytic therapy and clearing catheters.

Abstract: This invention describes a novel method of producing bioactive recombinant proteins from plants. General methods of designing and engineering plants for expression and production of such proteins are also disclosed. Methods for the expression of Transforming Growth Factor-&bgr; (TGF-&bgr;) proteins, such as Müllerian Inhibiting Substance (MIS), in plants, and methods of producing recombinant proteins from plants are specifically disclosed. Furthermore, the present invention provides methodology for the direct expression and production of a bioactive C-terminal fragment of a TGF-&bgr; protein, such as C-terminal MIS. The new method is more cost-effective than other large-scale expression systems, by eliminating the need for costly cell culture and fermentation manufacturing facilities.

Abstract: The invention relates to the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which transgenic tobacco plants having recombinant expression constructs comprising human hGC and IDUA nucleotide sequences produced enzymatically active modified human glucocerebrosidase and human .alpha.-L-iduronidase. The recombinant lysosomal enzymes produced in accordance with the invention may be used for a variety of purposes, including but not limited to enzyme replacement therapy for the therapeutic treatment of human and animal lysosomal storage diseases.