Patents by Inventor Karen Oishi
Karen Oishi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10472644Abstract: The present invention relates to protein expression in plants, particularly the large-scale production of recombinant polypeptides in whole Nicotiana tabacum plants. The use of preselected combination of N. tabacum varieties and Agrobacterium strains, optionally including one or more improvements to the transient expression-based methods of the invention, enables the production of large quantities of a heterologous polypeptides economically and in a short period of time.Type: GrantFiled: January 17, 2012Date of Patent: November 12, 2019Assignee: Philip Morris Products S.A.Inventors: Julien Beuchat, Prisca Campanoni, Shunhong Dai, Claudio Facchinetti, Nicolas Lugon-Moulin, Karen Oishi, Gustavo Ramirez, Sandrine Roesti, Helene Laparra, Rosa Cabrera
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Patent number: 9512439Abstract: The present invention relates to methods for expressing proteins of interest, particularly pharmaceutically valuable proteins, transiently in plants. In particular, the invention provides an improved method for introducing Agrobacterium cells into a whole plant or a plant organ. The methods of the invention provides efficient agroinfiltration of many plants singly or simultaneously resulting in a yield of recombinant proteins that is higher than that obtained by other methods. The methods can be readily scaled and automated to meet changing demands of the recombinant protein.Type: GrantFiled: July 15, 2011Date of Patent: December 6, 2016Assignee: Philip Morris Products S.A.Inventors: Andrea Carraro, John Faulkner, Yorick Klipfel, Oleg Mironov, Karen Oishi, Sandrine Roesti
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Patent number: 9056894Abstract: The present invention relates to a method for capturing virus-like particles of interest from a mixture comprising the use of an expanded bed of adsorbent; suitably wherein said method comprises the steps of: (a) providing an expanded bed of adsorbent; (b) contacting the mixture with the adsorbent such that the constituents of the mixture contact the expanded bed of adsorbent; (c) optionally washing the adsorbent; and (d) optionally eluting the particle of interest from the adsorbent.Type: GrantFiled: October 27, 2011Date of Patent: June 16, 2015Assignee: Philip Morris Products S.A.Inventors: Allan Lihme, Karen Oishi, Inga Vaarst, Rosa Cabrera
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Publication number: 20140371426Abstract: The present invention provides, in one aspect, a method for producing Apolipoprotein in a plant comprising incubating or growing a plant comprising a nucleic acid construct comprising, consisting or consisting essentially of a nucleic acid sequence encoding an Apolipoprotein fusion protein that comprises a fusion protein partner that induces the formation of a protein body in a plant, preferably, wherein the nucleic acid construct is introduced or infiltrated into the plant prior to the incubating or growing step.Type: ApplicationFiled: December 21, 2011Publication date: December 18, 2014Applicant: PHILIP MORRIS PRODUCTS S.A.Inventors: Karen Oishi, Manuel Peitsch
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Publication number: 20140302158Abstract: The present invention relates to a composition or composition of bilayer lipid vesicles displaying an influenza virus antigen and carbohydrate, and the production thereof in Nicotiana tabacum plants. Also disclosed herein are pharmaceutical compositions comprising said lipid vesicles for use in the treatment or prophylaxis of viral infections.Type: ApplicationFiled: November 12, 2012Publication date: October 9, 2014Applicant: Phillip Morris Products S.A.Inventors: Rosa Cabrera, Karen Oishi, Laurent Perez, Fabio Talamo
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Publication number: 20140298508Abstract: The present invention is directed to alpha-mannosidase sequences from plants and the use thereof, especially genomic nucleotide sequences containing the regulatory elements controlling their expression, intron and exon sequences and polynucleotide sequences coding for alpha-mannosidase enzymes. Such plants with modified alpha-mannosidase activity can be used for the production of glycoproteins having an altered saccharide composition of great benefit. The present invention also relates to the use of these alpha-mannosidase enzymes for hydrolyzing mannoses.Type: ApplicationFiled: December 23, 2011Publication date: October 2, 2014Applicant: PHILIP MORRIS PRODUCTS S.A.Inventors: Nikolai Valeryevitch Ivanov, Prisca Camponini, Dionisius Florack, Karen Oishi
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Publication number: 20140290135Abstract: The present invention relates to a system (1) for infiltrating plant tissue with macromolecules or microorganisms. The system comprises an infiltration chamber (2) having at least one sealable hatch for receiving plant tissue, means (P1) for reducing pressure within the infiltration chamber; a first release valve (V1) in fluid communication with the infiltration chamber. The system further comprises a controller (C1) for operating coordinately the first release valve and the pressure reducing means such that the pressure in the infiltration chamber is (i) reduced from a start pressure to a target pressure that is less than or equal to 300 mbar in a first time period, (ii) maintained at the target pressure for a second time period, and (iii) increased from the target pressure to a final pressure of at least approximately 1 bar within a third time period of less than or equal to five (5) seconds. The present invention also relates to a method of infiltrating plant tissue with bacterium cells.Type: ApplicationFiled: December 20, 2011Publication date: October 2, 2014Applicant: PHILIP MORRIS PRODUCTS S.A.Inventors: Andrea Carraro, Anne Mc Govern, Oleg Mironov, Karen Oishi, Sandrine Roesti
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Publication number: 20140296494Abstract: The present invention relates to protein expression in plants, particularly the large-scale production of recombinant polypeptides in whole Nicotiana tabacum plants. The use of preselected combination of N. tabacum varieties and Agrobacterium strains, optionally including one or more improvements to the transient expression-based methods of the invention, enables the production of large quantities of a heterologous polypeptides economically and in a short period of time.Type: ApplicationFiled: January 17, 2012Publication date: October 2, 2014Applicant: PHILIP MORRIS PRODUCTS S.A.Inventors: Julien Beuchat, Prisca Campanoni, Shunhong Dai, Claudio Facchinetti, Nicolas Lugon-Moulin, Karen Oishi, Gustavo Ramirez, Sandrine Roesti, Helene Laparra, Rosa Cabrera
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Publication number: 20140259215Abstract: The present invention provides, in one aspect, to a method for expressing DNase in a plant comprising growing a plant that has been transformed with a nucleic acid construct comprising a nucleic acid sequence encoding DNase under the control of a regulatory nucleotide sequence that regulates the transcription of said nucleic acid sequence in said plant.Type: ApplicationFiled: December 21, 2011Publication date: September 11, 2014Applicant: PHILIP MORRIS PRODUCTS S.A.Inventors: Karen Oishi, Manuel Peitsch
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Publication number: 20140081006Abstract: The present invention provides, in one aspect, a method for producing Mullerian Inhibitor Substance in a plant comprising incubating or growing a plant into which has been introduced or infiltrated a nucleic acid construct comprising, consisting or consisting essentially of a nucleic acid sequence encoding a Mullerian Inhibitor Substance fusion protein that comprises a fusion protein partner that induces the formation of a protein body in a plant, preferably, wherein the step of introducing or infiltrating the plant is performed prior to the incubating or growing step.Type: ApplicationFiled: December 21, 2011Publication date: March 20, 2014Applicant: PHILIP MORRIS PRODUCTS S.A.Inventor: Karen Oishi
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Publication number: 20130317197Abstract: The present invention relates to a method for capturing virus-like particles of interest from a mixture comprising the use of an expanded bed of adsorbent; suitably wherein said method comprises the steps of: (a) providing an expanded bed of adsorbent; (b) contacting the mixture with the adsorbent such that the constituents of the mixture contact the expanded bed of adsorbent; (c) optionally washing the adsorbent; and (d) optionally eluting the particle of interest from the adsorbent.Type: ApplicationFiled: October 27, 2011Publication date: November 28, 2013Applicant: PHILIP MORRIS PRODUCTS S.A.Inventors: Allan Lihme, Karen Oishi, Inga Vaarst, Rosa Cabrera
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Publication number: 20130205448Abstract: The present invention relates to methods for expressing proteins of interest, particularly pharmaceutically valuable proteins, transiently in plants. In particular, the invention provides an improved method for introducing Agrobacterium cells into a whole plant or a plant organ. The methods of the invention provides efficient agroinfiltration of many plants singly or simultaneously resulting in a yield of recombinant proteins that is higher than that obtained by other methods. The methods can be readily scaled and automated to meet changing demands of the recombinant protein.Type: ApplicationFiled: July 15, 2011Publication date: August 8, 2013Applicant: PHILIP MORRIS PRODUCTS S.A.Inventors: Andrea Carraro, John Faulkner, Yorick Klipfel, Oleg Mironov, Karen Oishi, Sandrine Roesti
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Publication number: 20060248616Abstract: This invention describes a novel method of producing bioactive recombinant proteins from plants. General methods of designing and engineering plants for expression and production of such proteins are also disclosed. Methods for the expression of Transforming Growth Factor-? (TGF-?) proteins, such as Müllerian Inhibiting Substance (MIS), in plants, and methods of producing recombinant proteins from plants are specifically disclosed. Furthermore, the present invention provides methodology for the direct expression and production of a bioactive C-terminal fragment of a TGF-? protein, such as C-terminal MIS. The new method is more cost-effective than other large-scale expression systems, by eliminating the need for costly cell culture and fermentation manufacturing facilities.Type: ApplicationFiled: November 8, 2005Publication date: November 2, 2006Inventors: Karen Oishi, Leonard Comaratta
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Publication number: 20060162026Abstract: Bioactive TGF-? proteins are expressed in transgenic plastids. The TGF-? proteins are used for therapeutic and diagnostic purposes. In particular, Mullerian Inhibitor Substance (MIS), either full length or truncated proteins, are expressed in plastids and used for treating various cancers that contain MIS receptors such as ovarian cancer, breast cancer and prostate cancer.Type: ApplicationFiled: December 23, 2005Publication date: July 20, 2006Inventors: Karen Oishi, David Williams