Abstract: The disclosure concerns a method for extracting spatial distribution statistics from patient tissue samples assayed with a tissue-based test for the purpose of scoring the patient tissue samples and guiding treatment based on the score(s). The method described herein utilizes digital image analysis of an image of one or more tissue sections to extract object-based (e.g., cells) features. The object-based features within the image of the tissue section is further processed using one or more algorithm processes to extract sophisticated spatial distribution features of one or more object type or sub-type in the tissue. Statistics describing the spatial distribution features are summarized to generate a patient-specific diagnostic score, and this score can be evaluated to guide patient treatment decisions.