Patents by Inventor Karim Tabiti
Karim Tabiti has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220205025Abstract: The present invention is directed to a method for determining of at least one microsatellite instability (MSI) based on a shift in a capillary electrophoresis (CE) profile (CE profile shift), the CE profile shift being determined by a comparison between the capillary electrophoresis (CE) profile of a target sequence of at least one microsatellite (MSI target profile) and the capillary electrophoresis (CE) profile of its specific wild type sequence (MS wild type profile). Further, the invention encompass suitable primer for use in said method, a kit comprising all essential components for performing said method successfully, a complete closed device as a system, namely “MSI Modaplex Analysis System” and a method for diagnosis of MSI phenotypes associated with an inflammation, cancer, inflammation associated cancer and/or auto immune disease, wherein the diagnosis comprises the method for determining of at least one CE profile shift as mentioned above.Type: ApplicationFiled: May 12, 2020Publication date: June 30, 2022Applicant: Biotype GmbHInventor: Karim Tabiti
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Patent number: 8744777Abstract: The present invention concerns a method for the quantification of a target nucleic acid in a sample comprising the following steps: (i) determination of the amplification efficiency of the target nucleic acid under defined amplification conditions, (ii) amplification of the target nucleic acid contained in the sample under the same defined reaction conditions, (iii) measuring the amplification in real-time, (iv) quantification of the original amount of target nucleic acid in the sample by correction of the original amount derived from step (iii) with the aid of the determined amplification efficiency. The efficiency correction of PCR reactions according to the invention for the quantification of nucleic acids can be used for absolute quantification with the aid of an external or internal standard as well as for relative quantification compared to the expression of housekeeping genes.Type: GrantFiled: June 17, 2011Date of Patent: June 3, 2014Assignee: Roche Molecular Systems, Inc.Inventors: Gregor Sagner, Karim Tabiti, Martin Gutekunst, Richie Soong
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Publication number: 20120270222Abstract: The present invention concerns a method for the quantification of a target nucleic acid in a sample comprising the following steps: (i) determination of the amplification efficiency of the target nucleic acid under defined amplification conditions, (ii) amplification of the target nucleic acid contained in the sample under the same defined reaction conditions, (iii) measuring the amplification in real-time, (iv) quantification of the original amount of target nucleic acid in the sample by correction of the original amount derived from step (iii) with the aid of the determined amplification efficiency. The efficiency correction of PCR reactions according to the invention for the quantification of nucleic acids can be used for absolute quantification with the aid of an external or internal standard as well as for relative quantification compared to the expression of housekeeping genes.Type: ApplicationFiled: June 17, 2011Publication date: October 25, 2012Applicant: ROCHE DIAGNOSTICS OPERATIONS, INC.Inventors: GREGOR SAGNER, KARIM TABITI, MARTIN GUTEKUNST, RICHIE SOONG
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Patent number: 8024132Abstract: The present invention concerns a method for the quantification of a target nucleic acid in a sample comprising the following steps: (i) determination of the amplification efficiency of the target nucleic acid under defined amplification conditions, (ii) amplification of the target nucleic acid contained in the sample under the same defined reaction conditions, (iii) measuring the amplification in real-time, (iv) quantification of the original amount of target nucleic acid in the sample by correction of the original amount derived from step (iii) with the aid of the determined amplification efficiency. The efficiency correction of PCR reactions according to the invention for the quantification of nucleic acids can be used for absolute quantification with the aid of an external or internal standard as well as for relative quantification compared to the expression of housekeeping genes.Type: GrantFiled: August 27, 2009Date of Patent: September 20, 2011Assignee: Roche Molecular SystemsInventors: Gregor Sagner, Karim Tabiti, Martin Gutekunst, Richie Soong
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Publication number: 20100021923Abstract: The present invention concerns a method for the quantification of a target nucleic acid in a sample comprising the following steps: (i) determination of the amplification efficiency of the target nucleic acid under defined amplification conditions, (ii) amplification of the target nucleic acid contained in the sample under the same defined reaction conditions, (iii) measuring the amplification in real-time, (iv) quantification of the original amount of target nucleic acid in the sample by correction of the original amount derived from step (iii) with the aid of the determined amplification efficiency. The efficiency correction of PCR reactions according to the invention for the quantification of nucleic acids can be used for absolute quantification with the aid of an external or internal standard as well as for relative quantification compared to the expression of housekeeping genes.Type: ApplicationFiled: August 27, 2009Publication date: January 28, 2010Applicant: Roche Molecular Systems, Inc.Inventors: Gregor Sagner, Karim Tabiti, Martin Gutekunst, Richie Soong
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Patent number: 7541448Abstract: The invention is directed to an oligonucleotide comprising a molecular rod, wherein preferentially the molecular rod covalently connects the 3? end of a first nucleotide residue with the 5? end of a second nucleotide residue. Such oligonucleotides are highly advantageous for real time PCR melting curve analysis.Type: GrantFiled: December 2, 2004Date of Patent: June 2, 2009Assignee: Roche Diagnostis Operations, Inc.Inventors: Dieter Heindl, Astrid Reiser, Karim Tabiti, Martina Junius, Alexander Hauber
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Patent number: 7378241Abstract: The present invention concerns a method for determining the efficiency of the amplification of a target nucleic acid comprising the following steps: (i) preparation of a dilution series of the target nucleic acid, (ii) amplifying the target nucleic acid under defined reaction conditions, the amplification being measured in real-time (iii) setting a defined signal threshold value, (iv) determining the cycle number at which the signal threshold value is exceeded for various dilutions, (v) determining the amplification efficiency as a function of the amount of original target nucleic acid. The present invention also concerns a method for the quantification of a target nucleic acid in a sample in which the efficiency of the amplification reaction is determined in this manner and is taken into account in the quantification.Type: GrantFiled: March 25, 2004Date of Patent: May 27, 2008Assignee: Roche Diagnostics Operations, Inc.Inventors: Gregor Sagner, Karim Tabiti, Martin Gutekunst, Richie Soong
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Patent number: 7125691Abstract: The present invention concerns a method for determining the efficiency of the amplification of a target nucleic acid comprising the following steps: (i) preparation of a dilution series of the target nucleic acid, (ii) amplifying the target nucleic acid under defined reaction conditions, the amplification being measured in real-time (iii) setting a defined signal threshold value, (iv) determining the cycle number at which the signal threshold value is exceeded for various dilutions, (v) determining the amplification efficiency as a function of the amount of original target nucleic acid. The present invention also concerns a method for the quantification of a target nucleic acid in a sample in which the efficiency of the amplification reaction is determined in this manner and is taken into account in the quantification.Type: GrantFiled: March 30, 2001Date of Patent: October 24, 2006Assignee: Roche Molecular Systems, Inc.Inventors: Gregor Sagner, Karim Tabiti, Martin Gutekunst, Richie Soong
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Patent number: 7118867Abstract: The invention provides a method for quantitative multiplex PCR of two targets whereby one target is present in at least 100-fold molar excess over that of the other target. The method further comprises the addition of a thermostable DNA polymerase with a final concentration of at least 0.5 units/?l.Type: GrantFiled: November 19, 2002Date of Patent: October 10, 2006Assignees: Roche Diagnostics Corporation, Idaho Technology, Inc.Inventors: Karim Tabiti, Gisela Betzl, Richie Soong, Randy P. Rasmussen, Deepika Marine Desilva, John G. Ward, Hallegh Page Millward
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Publication number: 20050142598Abstract: The invention is directed to an oligonucleotide comprising a molecular rod, wherein preferentially the molecular rod covalently connects the 3? end of a first nucleotide residue with the 5? end of a second nucleotide residue. Such oligonucleotides are highly advantageous for real time PCR melting curve analysis.Type: ApplicationFiled: December 2, 2004Publication date: June 30, 2005Inventors: Dieter Heindl, Astrid Reiser, Karim Tabiti, Martina Junius, Alexander Hauber
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Publication number: 20050009048Abstract: The present invention concerns a method for determining the efficiency of the amplification of a target nucleic acid comprising the following steps: (i) preparation of a dilution series of the target nucleic acid, (ii) amplifying the target nucleic acid under defined reaction conditions, the amplification being measured in real-time (iii) setting a defined signal threshold value, (iv) determining the cycle number at which the signal threshold value is exceeded for various dilutions, (v) determining the amplification efficiency as a function of the amount of original target nucleic acid. The present invention also concerns a method for the quantification of a target nucleic acid in a sample in which the efficiency of the amplification reaction is determined in this manner and is taken into account in the quantification.Type: ApplicationFiled: March 25, 2004Publication date: January 13, 2005Inventors: Gregor Sagner, Karim Tabiti, Martin Gutekunst, Richie Soong
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Publication number: 20040153254Abstract: The present invention concerns a method for the quantification of a target nucleic acid in a sample comprising the following steps: (i) determination of the amplification efficiency of the target nucleic acid under defined amplification conditions, (ii) amplification of the target nucleic acid contained in the sample under the same defined reaction conditions, (iii) measuring the amplification in real-time, (iv) quantification of the original amount of target nucleic acid in the sample by correction of the original amount derived from step (iii) with the aid of the determined amplification efficiency. The efficiency correction of PCR reactions according to the invention for the quantification of nucleic acids can be used for absolute quantification with the aid of an external or internal standard as well as for relative quantification compared to the expression of housekeeping genes.Type: ApplicationFiled: December 24, 2003Publication date: August 5, 2004Applicant: Roche Molecular Systems, Inc.Inventors: Gregor Sagner, Karim Tabiti, Martin Gutekunst, Richie Soong
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Patent number: 6691041Abstract: The present invention concerns a method for the quantification of a target nucleic acid in a sample comprising the following steps: (i) determination of the amplification efficiency of the target nucleic acid under defined amplification conditions, (ii) amplification of the target nucleic acid contained in the sample under the same defined reaction conditions, (iii) measuring the amplification in real-time, (iv) quantification of the original amount of target nucleic acid in the sample by correction of the original amount derived from step (iii) with the aid of the determined amplification efficiency. The efficiency correction of PCR reactions according to the invention for the quantification of nucleic acids can be used for absolute quantification with the aid of an external or internal standard as well as for relative quantification compared to the expression of housekeeping genes.Type: GrantFiled: March 30, 2001Date of Patent: February 10, 2004Assignee: Roche Molecular Systems, Inc.Inventors: Gregor Sagner, Karim Tabiti, Martin Gutekunst, Richie Soong
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Publication number: 20030215830Abstract: The new invention provides a method and a kit for quantitative multiplex PCR with a high dynamic range characterized in that a thermostable DNA Polymerase with a final concentration of at least 0.5 units/&mgr;l is use.Type: ApplicationFiled: November 19, 2002Publication date: November 20, 2003Inventors: Karim Tabiti, Gisela Betzl, Richie Soong, Randy P. Rasmussen, Deepika Marine Desilva, John G. Ward, Haleigh Page Millward
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Publication number: 20030165832Abstract: The present invention concerns a method for the quantification of a target nucleic acid in a sample comprising the following steps: (i) determination of the amplification efficiency of the target nucleic acid under defined amplification conditions, (ii) amplification of the target nucleic acid contained in the sample under the same defined reaction conditions, (iii) measuring the amplification in real-time, (iv) quantification of the original amount of target nucleic acid in the sample by correction of the original amount derived from step (iii) with the aid of the determined amplification efficiency. The efficiency correction of PCR reactions according to the invention for the quantification of nucleic acids can be used for absolute quantification with the aid of an external or internal standard as well as for relative quantification compared to the expression of housekeeping genes.Type: ApplicationFiled: March 30, 2001Publication date: September 4, 2003Inventors: Gregor Sagner, Karim Tabiti, Martin Gutekunst, Richie Soong
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Publication number: 20020058262Abstract: The present invention concerns a method for determining the efficiency of the amplification of a target nucleic acid comprising the following steps: (i) preparation of a dilution series of the target nucleic acid, (ii) amplifying the target nucleic acid under defined reaction conditions, the amplification being measured in real-time (iii) setting a defined signal threshold value, (iv) determining the cycle number at which the signal threshold value is exceeded for various dilutions, (v) determining the amplification efficiency as a function of the amount of original target nucleic acid. The present invention also concerns a method for the quantification of a target nucleic acid in a sample in which the efficiency of the amplification reaction is determined in this manner and is taken into account in the quantification.Type: ApplicationFiled: March 30, 2001Publication date: May 16, 2002Inventors: Gregor Sagner, Karim Tabiti, Martin Gutekunst, Richie Soong
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Patent number: 6270965Abstract: The present invention concerns a method and a device which enables an integrated amplification and detection of nucleic acids.Type: GrantFiled: July 15, 1998Date of Patent: August 7, 2001Assignee: Roche Diagnostics, GmbHInventors: Jörg Kleiber, Karim Tabiti, Gregor Sagner
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Patent number: 5888746Abstract: A method for diagnosis or prognosis of a cancer is disclosed. The method comprises (i) detecting in a first biological sample protein tyrosine phosphate .alpha. (PTP.alpha.) or PTP.alpha. nucleic acid, and (ii) comparing the level of PTP.alpha. or PTP.alpha. nucleic acid in the first sample with the level in a second biological sample known to be from normal tissue. Any overexpression of PTP.alpha. or PTP.alpha. nucleic acid in the first sample compared to the second sample is indicative that the first sample is from a cancerous tissue.Type: GrantFiled: March 10, 1997Date of Patent: March 30, 1999Assignee: Institute of Molecular and Cell BiologyInventors: Karim Tabiti, Catherine Jane Pallen