Abstract: Methods for determining glycation on a molecule and/or a glycan structure on a glycosylated molecule through the use of a combination of spectroscopic analysis and chemometric modeling are described. In addition, methods and systems for producing a molecule with a desired level of glycation, including a non-glycated molecule, and/or a desired level of a glycan structure on a glycosylated molecule are described.

Abstract: Methods for determining glycation on a molecule and/or a glycan structure on a glycosylated molecule through the use of a combination of spectroscopic analysis and chemometric modeling are described. In addition, methods and systems for producing a molecule with a desired level of glycation, including a non-glycated molecule, and/or a desired level of a glycan structure on a glycosylated molecule are described.

Abstract: Disclosed is a method of quantifying virus titer in a sample using Raman spectroscopy. This method comprises providing a sample; providing a model for determining viral titer in the sample; irradiating the sample with a light source; and acquiring a Raman spectrum of the sample. The method further involves quantifying the viral titer of the sample by applying a virus component of the Raman spectrum to the model for determining viral titer. Other aspects of the disclosure relate to a method for generating a model suitable for quantifying viral titer in a sample.

Abstract: A method and device are provided for concentrating and separating materials in fluids within a fluidic device having a fluid conduit such as a channel or capillary. The concentration is achieved by balancing the electrophoretic velocity of a material against the bulk flow of fluid in the presence of a temperature gradient. An additive is added to the fluid which interacts with the material and which modifies the normal electrophoretic mobility of the material. Using an appropriate fluid, the temperature gradient can generate a corresponding gradient in the electrophoretic velocity so that the electrophoretic and bulk velocities sum to zero at a unique position along the conduit and the material will be focused at that position. The method and device may be adapted for use with a variety of materials including fluorescent dyes, amino acids, proteins, DNA and to concentrate a dilute material.

Abstract: A method is provided for observing mixing interactions and reactions of two materials in a fluid. The method in one form provides for concentrating by balancing electrophoretic velocities of a material against the bulk flow of fluid in the presence of a temperature gradient. Using an appropriate fluid, the temperature gradient can generate a corresponding gradient in the electrophoretic velocity of the material so that the electrophoretic and bulk velocities sum to zero at a unique position and the material will be focused at that position. A second material can then be introduced into the fluid and allowed to move through and interact with the focused band of the first material. Products of the interaction can then be detected as they are focused at a different position along the gradient. The method can be adapted to study the temperature dependence of the molecular interaction.