Abstract: Electrically controlled hybridization is used to selectively assemble oligonucleotides on the surface of a microelectrode array. Controlled activation of individual electrodes in the microelectrode array attracts oligonucleotides in solution to specific regions of the array where they hybridize to other oligonucleotides anchored on the array. The oligonucleotides that hybridize may provide locations for subsequent oligonucleotides to hybridize. The active electrodes and the oligonucleotides in solution may be varied during each round of synthesis. This allows for multiple oligonucleotides each with different and specific sequences to be created in parallel. This is accomplished without the use of phosphoramidite chemical synthesis or template-independent DNA polymerase enzymatic synthesis. Oligonucleotides created with these techniques may be used to encode digital data. Fully assembled oligonucleotides may be separated from the array and sequenced, stored, or otherwise processed.

Abstract: De novo polynucleotide synthesis is performed with a substrate-bound polymerase. The polymerase is attached to a solid substrate such as a microelectrode array. The polymerase adds nucleotides to growing polynucleotides strands that are also attached to the solid substrate. Spatial control of polymerase activity is achieved by changing the rate of nucleotide polymerization at selected locations on the surface of the solid substrate. The rate of polymerization is changed by inhibiting or promoting activity of the polymerase. In some implementations, activation of electrodes in the microelectrode array changes the rate of nucleotide polymerization. Nucleotides are added to the growing polynucleotide strands at areas where the polymerase is active. By varying the locations where the substrate-bound polymerase is active and the species of nucleotide added, a population of polynucleotides with different, arbitrary sequences is synthesized on the surface of the solid substrate.

Abstract: A sterilizer apparatus for treating liquids or gasses, utilizing the low wavelength, ozone producing, vacuum UV wavelengths, utilizing a novel reactor chamber built around an ultraviolet lamp operating in the presence of either a vacuum or an oxygen-free medium.

Abstract: A method of rendering a glyph of a vector-based font comprises the steps of: generating a MIP map for a glyph, where the MIP map comprises at least one level having a plurality of pixels and where each level is generated from original vector data for the glyph; and storing the MIP map on a computer readable medium. The method further comprises placing the glyph in an image to be rendered; retrieving the stored MIP map for the glyph; rendering a level of the MIP map, where the level is associated with a resolution of the glyph; and displaying the glyph as the rendered level.