Abstract: A method of depolymerizing a polymer by combining together the polymer with an enzyme (a hydrolytic enzyme capable of catalyzing cleavage of said (CO)—O bond of an ester or carbonate linkage of the polymer) and an aqueous liquid to provide a reaction mixture. The polymer comprises a (CO)—O bond of an ester or carbonate linkage. The reaction mixture defines a ratio eta (?) of liquid volume, in ?L, to total solids, in mg, that is less than 2 ?L/mg. Then, allowing an enzyme-catalyzed reaction of the enzyme with the polymer to take place thereby forming a reaction product.

Abstract: A method of depolymerizing a polymer by combining together the polymer with an enzyme (a hydrolytic enzyme capable of catalyzing cleavage of said (CO)—O bond of an ester or carbonate linkage of the polymer) and an aqueous liquid to provide a reaction mixture. The polymer comprises a (CO)—O bond of an ester or carbonate linkage. The reaction mixture defines a ratio eta (?) of liquid volume, in ?L, to total solids, in mg, that is less than 2 ?L/mg. Then, allowing an enzyme-catalyzed reaction of the enzyme with the polymer to take place thereby forming a reaction product.

Abstract: A method for the enzymatic saccharification of a polysaccharide is provided. This method comprises the step a) of contacting the polysaccharide with a hydrolase and water, in the absence of solvent, thereby forming a solid reaction mixture; and the step b) of: b)-i. mixing and then incubating the solid reaction mixture, b)-ii. milling the solid reaction mixture, or b)-iii. milling and then incubating the solid reaction mixture.

Abstract: This invention relates to a compound of Formula 1A: or a pharmaceutically acceptable salt thereof; wherein R1 to R4, R7, R9 to R12 and Y are as defined herein. The invention further relates to pharmaceutical composition comprising said compound or a pharmaceutically acceptable salt thereof; and method of using same for reducing or reversing bacterial resistance to at least one aminoglycoside antibiotic.

Abstract: This invention relates to a compound of Formula 1A: or a pharmaceutically acceptable salt thereof; wherein R1 to R4, R7, R9 to R12 and Y are as defined herein. The invention further relates to pharmaceutical composition comprising said compound or a pharmaceutically acceptable salt thereof; and method of using same for reducing or reversing bacterial resistance to at least one aminoglycoside antibiotic.

Abstract: The present invention relates to novel inhibitors of aminoglycoside 6?-N-acetyltransferases, more specifically, AAC(6?)-li and AAC(6?)-ly, as well as compositions and uses thereof. Furthermore, the present invention relates to synthetic methodologies for preparing the inhibitors of aminoglycoside 6?-N-acetyltransferases.

Abstract: The present invention relates to inhibitors of aminoglycoside 6?-N-acetyltransferases of Formula I: R—X—Y-Z??Formula I wherein: R is selected from the group consisting of: R1 is selected from the group consisting of OH and R2 is selected from the group consisting of OH and R3 is selected from the group consisting of NH2 and OH; R4 is selected from the group consisting of NH2 and R5 is selected from the group consisting of OMe, OEt OPr, and O-iPr; X is selected from the group consisting of NH and O; Y is selected from the group consisting of: R6 is selected from the group consisting of OH, CH3, and OCH3; n is an integer ranging from 1 to 10; and Z is selected from the group consisting of: and R7 is selected from the group consisting of OH, OMe, OEt OPr, O-iPr, O-tBu and