Abstract: The invention relates to monoalkylaminoketones of the formula (I), in which R1 and R2 have the meanings indicated.

Abstract: The invention relates to monoalkylaminoketones of the formula (I), in which R1 and R2 have the meanings indicated.

Abstract: In a method for immobilizing biomolecules and affinity ligands water insoluble matrices, having amino groups and selected from test tubes, microtiter plates, microscope slides, beads, membranes, resins, and filters, are reacted with a cyclobutene carboxylic acid derivative, such as cyclobutene carboxylic acid diester, cyclobutene carboxylic acid halide, cyclobutene carboxylic acid ester halide, cyclobutene carboxylic acid dialkoxyester, and cyclobutene carboxylic acid imidazole, as an activating compound in methanol and triethylamine to form active matrices with active groups. A protein containing at least one primary or secondary amino group, is dissolved and added to the matrices. The activated matrices and the dissolved protein are incubated at pH of 7-10 and a temperature of +4° C. to +60° C. in an aqueous buffer system, free of primary and secondary amines, to thereby immobilize the protein on the matrices.

Abstract: The present invention related to heterobifunctional cross-linkers substituted by a cell surface binding group or a cell recognising group, and also by a bio-active group, the bio-active group attached to a carbonyl of the cross-linker by an acid labile bond. Examples of suitable bio-active groups include an immunomodulator and an anti-neoplastic group. The conjugate enables, for example, the coupling of immunomodulators ex vivo to a target cell, for example a tumor antigen presenting cell. The conjugate can act as a non-toxic prodrug carrying a cytotoxin, for example, to a target tissue site, where the pH-dependency of the labile amide bond provides for cell selectivity of the masked cytotoxin. The invention further relates to a method for producing the cross-linkers, their use in treating disease, and ex-vivo cell labeling.

Abstract: A method for analyzing the repair of DNA modifications and base mispairings as well as apurinic and apyrimidinic sites by DNA repair enzymes, comprising the following steps: contacting (a) single- or double-stranded DNA molecules which were covalently coupled to a solid-phase matrix carrying primary or secondary amino groups by reaction with a reactive squaric acid derivative, and which have modifications and/or base mispairings and/or apurinic or apyrimidinic sites, with (b) a composition containing DNA repair enzymes; and determining the elimination of the DNA modifications and/or base mispairings and/or apurinic or apyrimidinic sites. The DNA molecules are covalently coupled to the solid state matrix via a primary or secondary amino group incorporated in the DNA molecule at the 5′-end or at the 3′-end of the DNA or in the 2′-position of at least one deoxyribosyl residue.