Abstract: A system and method of automated incubating and reading of biological indicators is disclosed. In one embodiment, the automated biological indicator incubator is configured to control the temperature of incubation test wells to a desired temperature range suitable for use with a biological indicator, control the incubation period, and detect a change in the biological indicator colored media providing an indication of growth or lack of growth. In other embodiments, the automated biological indicator incubator is self-calibrating, and provides a communication interface to an external device, such as a computer. The communication interface is suitable for use to collect and analyze data associated with the biological indicator during the incubator period, and for making a determination of growth or lack of growth and success of the sterilization process as well as a permanent document of the monitoring of the sterilization process.