Abstract: The present invention provides methods, compositions, and systems for distributing molecules and complexes into reaction sites. In particular, the methods, compositions, and systems of the present invention result in loading of polymerase enzyme complexes into a predetermined number of reaction sites, including nanoscale wells.

Abstract: Embodiments of the present disclosure include a system for directing the flow of fluids via a production tree. The production tree extends upwardly from a lower surface to an upper surface and at least partially enclosing an upper section of a production bore and an upper section of an annulus bore, wherein one of a lower section and the upper section of the production bore includes a first production valve, the upper section of the production bore includes a second production valve, and the upper section of the annulus bore includes a second annulus valve.

Abstract: The present invention provides methods, compositions, and systems for distributing molecules and complexes into reaction sites. In particular, the methods, compositions, and systems of the present invention result in loading of polymerase enzyme complexes into a predetermined number of reaction sites, including nanoscale wells.

Abstract: A control system is operable to control an autonomous machine for autonomously applying one or more products to a jobsite in accordance with a set of operation instructions. The products are stored in one or more interchangeable containers which are initially located on a station. The control system broadly includes an alignment sensor and a machine processor. The alignment sensor is configured to sense and generate alignment data regarding a relative position of the station and a particular container that contains a particular product.

Abstract: A control system is operable to control an autonomous machine for autonomously applying one or more products to a jobsite in accordance with a set of operation instructions. The products are stored in one or more interchangeable containers which are initially located on a station. The control system broadly includes an alignment sensor and a machine processor. The alignment sensor is configured to sense and generate alignment data regarding a relative position of the station and a particular container that contains a particular product.

Abstract: An agricultural system includes an agricultural product supply station, an autonomous agricultural machine, a plurality of product containers, and a powered transfer mechanism. The machine is operable to advance across a field while dispensing product. The product containers are configured to be removably stored by the supply station in a stored position and used interchangeably with the machine. Each container is configured to contain product and operably couple to the machine to dispense product thereto. Each container is shiftable between a machine-coupled position and the stored position. The container is attached to the machine in the machine-coupled position and detached from the machine in the stored position. The transfer mechanism is operably supported by at least one of the supply station and the implement and is configured to shift each container between the machine-coupled and stored positions.

Abstract: An autonomous agricultural implement is operable to advance itself across a field while dispensing agricultural product. The implement includes an implement chassis, a drive system, an interchangeable product container, and a plurality of openers. The container presents a chamber configured to hold a supply of product. The container is shiftable between coupled and uncoupled positions, with the container being detached from the implement chassis in the uncoupled position. The container includes a product flow barrier to selectively restrict product from flowing out of the chamber when the container is in the uncoupled position prior to the container being attached to the implement chassis. The container fluidly communicates with the openers when in the coupled position so that the openers receive product from the chamber.

Abstract: A mobile agricultural product dispensing system is configured to hold a supply of agricultural product and transport the product for operations on a field by an agricultural machine. The dispensing system includes a wheeled supply station and a plurality of product containers. The supply station is operable to be advanced to and from the field and includes a rack assembly defining a series of container-receiving bays. The product containers are configured to be used interchangeably with the machine. Each container is configured to contain product and operably couple to the machine to dispense product thereto. Each container is removably supported by the rack assembly in a corresponding one of the bays.

Abstract: A control system for a subsea well is provided. The control system comprises a tree comprising a hydraulic control supply line for use in opening a downhole safety valve as a result of hydraulic pressure in the line. A part of the line is carried by a structure which is subject to the pressure of a production fluid from the well used in the control system so that the line is separable in response to a failure of the integrity of the structure.

Abstract: A control system for a subsea well is provided. The control system comprises a tree comprising a hydraulic control supply line for use in opening a downhole safety valve as a result of hydraulic pressure in the line. A part of the line is carried by a structure which is subject to the pressure of a production fluid from the well used in the control system so that the line is separable in response to a failure of the integrity of the structure.

Abstract: A subsea wellhead assembly positions a flow module between an outboard flowline of a subsea tree and a tubing hanger spool flowline. The subsea production assembly includes a wellhead, a tubing hanger spool disposed on the wellhead, and a subsea tree disposed on the tubing hanger spool. Production fluid may flow from the wellhead through the tubing hanger spool, and then through the tree. A tubing hanger spool frame mounts to and extends laterally from the tubing hanger spool and supports a downstream flowline having an upward facing hub. A tree frame mounts to and extends laterally from the subsea tree and supports an upstream flowline having an upward facing hub. A flow module having downward facing flowlines that couple to the hubs so that the weight of the flow module is distributed between the frames of the tubing hanger spool and the subsea tree.

Abstract: Methods and devices are described for concentration and cleanup of samples containing bio-molecule analytes (e.g., polynucleotides, such as DNA, RNA, PNA). Various embodiments provide for pH-mediated sample concentration and cleanup of nucleic acid samples with channel devices (e.g., cross-T format, microchannel devices).

Abstract: A method, apparatus, and electronic device for displaying data are disclosed. A non-rectangular addressable liquid crystal display 402 with a non-rectangular active area active matrix 334 may display an adjustable presentation of data. A display-integrated control key may control the presentation of data. A scrolling navigation control may scroll through the adjustable presentation of the data.

Abstract: Compositions and methods of use are disclosed for the analysis of polynucleotides isolated in individual reaction compartments.

Abstract: A method, apparatus, and electronic device for displaying data are disclosed. A non-rectangular addressable liquid crystal display 402 with a non-rectangular active area active matrix 334 may display an adjustable presentation of data. A display-integrated control key may control the presentation of data. A scrolling navigation control may scroll through the adjustable presentation of the data.

Abstract: Methods and apparatus are provided to resolve analytes within a fluid path using isoelectric focusing, gel electrophoresis, or other separation means. Materials within the fluid path that are compatible with these separation means are used to attach resolved analytes to the wall of the fluid path. Attachment results from a triggerable event such as photoactivation, thermal activation, or chemical activation. In accordance with a further aspect of the present invention, the material in the capillary may also be disrupted, by either the triggerable event or a subsequent event such as melting or photocleavage. Thus, an open lumen or porous structure may be created within the fluid path, allowing unbound analyte materials to be washed from the fluid path, and detection agents to be washed into the fluid path. The separation-compatible materials may be polymerizable monomers, gels, entangled polymers or other materials.

Abstract: Various embodiments provide, for example, buffer compositions and/or sieving formulations useful in connection with electrophoresis instruments, such as capillary electrophoresis (CE) devices. In various embodiments, a buffer composition can include Bis-Tris, TAPS and/or TAPSO, and, optionally, a chelating agent, such as EDTA. Methods of separating samples containing bio-molecules, such as DNA or RNA, are also described.

Abstract: Various embodiments provide, for example, buffer compositions and/or sieving formulations useful in connection with electrophoresis instruments, such as capillary electrophoresis (CE) devices. In various embodiments, a buffer composition can include Bis-Tris, TAPS and/or TAPSO, and, optionally, a chelating agent, such as EDTA. Methods of separating samples containing bio-molecules, such as DNA or RNA, are also described.

Abstract: Provided herein are methods and compositions for fluorogenic detection of a polyphosphate released from a nucleoside polyphosphate by the enzymatic action of a nucleic acid polymerase. The methods and compositions are based on the transfer of a free polyphosphate (or polyphosphate labeled with a fluorescence dye) to a polyphosphate acceptor molecule that is modified with a moiety that facilitates fluorogenic detection of a product that indicates the release of polyphosphate by the enzymatic action of a DNA or RNA polymerase. In one aspect, fluorogenic detection is facilitated by forming a fluorescent donor/acceptor pair on the polyphosphate acceptor substrate. The combination of the donor and acceptor pair provides a differentially detectable fluorescent product. In another aspect, fluorogenic detection is facilitated by releasing a fluorescent dye from the polyphosphate acceptor substrate when the free polyphosphate is transferred to the acceptor substrate.

Abstract: Methods and devices are described for concentration and cleanup of samples containing bio-molecule analytes (e.g., polynucleotides, such as DNA, RNA, PNA). Various embodiments provide for pH-mediated sample concentration and cleanup of nucleic acid samples with channel devices (e.g., cross-T format, microchannel devices).