Abstract: Microfluidic devices and methods for analyzing glycan profiles of glycoproteins are provided. Some embodiments of the devices comprise a deglycosylation column for cleaving glycans, an optional cleaning column for removing proteins, a trapping column for enriching glycans, and a separation column for resolving glycans. The devices and methods significantly improve the speed and sensitivity of glycan analysis.

Abstract: Microfluidic devices and methods for analyzing glycan profiles of glycoproteins are provided. Some embodiments of the devices comprise a deglycosylation column for cleaving glycans, an optional cleaning column for removing proteins, a trapping column for enriching glycans, and a separation column for resolving glycans. The devices and methods significantly improve the speed and sensitivity of glycan analysis.

Abstract: Aspects of the invention include a microfluidic device having a fluid flow path and a monolithic chromatographic material covalently bound to at least a portion of a polymeric surface of the fluid flow path. Aspects of the invention further include methods of making and using such devices.

Abstract: Aspects of the invention include a microfluidic device having a fluid flow path and a monolithic chromatographic material covalently bound to at least a portion of a polymeric surface of the fluid flow path. Aspects of the invention further include methods of making and using such devices.

Abstract: A microfluidic device, a device including the microfluidic device and methods of operation are described.

Abstract: Embodiments of sensors according to the invention include a substrate having an activated dielectric surface; a metal ion chelate layer on the activated dielectric surface; and a bifunctional layer on a surface of said metal ion chelate layer, where the bifunctional layer includes bifunctional molecules that have a metal ion affinity moiety; and a ligand attachment moiety. Ligands are located at one or more positions on the surface of the bifunctional layer in certain embodiments. Aspects of the invention further include methods of making the sensors, as well as kits for practicing the methods. Further aspects of the invention include methods of using the sensors, e.g., in analyte detection applications.

Abstract: Aspects of the invention include a microfluidic device having a fluid flow path and a monolithic chromatographic material covalently bound to at least a portion of a polymeric surface of the fluid flow path. Aspects of the invention further include methods of making and using such devices.

Abstract: Compounds, compositions, methods for sequencing proteins and peptides, and methods for identifying proteins and peptides in a mixture, are disclosed. Compounds of formula A-B-C wherein A is a nucleophilic reactive group, B is a detectable moiety capable of being isotopically labeled, and C is a charge replacement group, are used to label the peptides at the N-terminus or the C-terminus. The tagged peptides can then be analyzed by mass spectroscopy.

Abstract: The invention in particular embodiments provides an evanescent wave sensor which includes a ligand bound to a sensor substrate via a NCYX linker moiety, as defined herein. Methods of making the subject evanescent wave sensors are also provided which include contacting a first reactive moiety which has an isocyanato (or isothiocyanato) moiety with a second reactive moiety which has an hydroxyl, thiol, or amino moiety, as further described herein. Also provided by the invention are methods in which a subject evanescent wave sensor is contacted with a sample, and binding of analytes in the sample to the sensor is assessed by evanescent wave detection. The invention also provides kits and systems for performing the subject methods.

Abstract: A method useful in the analysis of glycosylated proteins, in which a mixture containing glycosylated proteins and unglycosylated proteins is contacted with a resin that includes a nucleophile bound to a solid support via a linker. The contacting is performed under conditions sufficient to result in removal of the glycosyl group from the glycosylated proteins and to concomitantly result in the deglycosylated proteins covalently bound to the solid support. The deglycosylated proteins bound to the solid support may be rinsed to remove proteins that are not covalently bound to the solid support. The deglycosylated proteins are released from the solid support and may be subjected to further purification and/or analysis.

Abstract: The present invention provides methods of detecting and/or isolating immune complexes. The antigen in the immune complexes can further be isolated from the immune complexes to facilitate research and characterization of the antigen. Other embodiments of the present invention can be used to, inter alia, develop assays for detecting immune complexes, diagnose diseases or medical conditions, as well as determine the correlation between the presence of immune complexes and diseases and medical conditions of interest. Still other embodiments provide reagents, equipments and kits for implementing the methods.

Abstract: The present invention is a method, and a kit for carrying out the method, of separating phosphorylated peptides from a mixture of phosphorylated and unphosphorylated peptides. The method involves reacting a collection of peptides, in which some of the peptides have one or more phosphate groups, with a first resin. The peptides are then reacted with a capture ligand which reacts with the phosphate group of the phosphorylated peptides to form a bond. The capture ligand is used to separate the phosphorylated peptides from the unphosphorylated peptides.

Abstract: Methods and compositions for modifying the surface of a polymeric substrate are provided. In the subject methods, at least a portion of the surface of the polymeric substrate is contacted with a biofouling resistant surfactant composition. At least a portion of the surfactant molecules of the composition are end modified with a non-reactive, charged end group that is charge stable over a pH range of about 2 to 12. The subject methods and compositions find particular use with electrophoretic devices, such as capillary electrophoretic devices.

Abstract: The present invention provides methods for analyzing a peptide or peptides of interest in a protein sample using a combination of a relatively generic isotope tag with a decoupled selection process, allowing simplified customization of the application with a single reagent. These methods comprise providing a first and a second protein sample; labeling the first protein sample with a first Universal Peptide Isotope Tag (U-PIT) reagent and the second protein sample with a second U-PIT reagent; separating the peptide of interest from the combined first and second protein samples; and determining the relative amount of the first U-PIT reagent and the second U-PIT reagent bound to the peptide or peptides of interest. The U-PIT label of the present inventive methods has the following general formula A-B-C wherein A is a nucleophilic reactive group, B is a detectable moiety that can be isotopically labeled, and C is a charge replacement group.

Abstract: Methods for selectively separating at least one component from a multi-component componant fluidic sample are provided. In the subject methods, the fluidic sample is introduced into a micro-fluidic device that includes at least one micro-valve made up of a phase reversible material. The multi-component fluidic sample is then contacted with the microvalve in a microfluidic device under conditions sufficient for the at least one component to enter the microvalve, while the remaining constituents of the fluidic sample remain outside of the microvalve. Also provided are kits for use in practicing the subject methods, where the kits include at least a microfluidic device having a microvalve and instructional material (or means for obtaining the same) on how to use the device in the subject methods. The subject devices find use in a variety of applications, including sample desalting and concentration applications.

Abstract: Compounds, compositions, methods for sequencing proteins and peptides, and methods for identifying proteins and peptides in a mixture, are disclosed. Compounds of formula A-B-C wherein A is a nucleophilic reactive group, B is a detectable moiety capable of being isotopically labeled, and C is a charge replacement group, are used to label the peptides at the N-terminus or the C-terminus. The tagged peptides can then be analyzed by mass spectroscopy.

Abstract: Methods and compositions for modifying the surface of a polymeric substrate are provided. In the subject methods, at least a portion of the surface of the polymeric substrate is contacted with a biofouling resistant surfactant composition. At least a portion of the surfactant molecules of the composition are end modified with a non-reactive, charged end group that is charge stable over a pH range of a bout 2 to 12. The subject methods and compositions find particular use with electrophoretic devices, such as capillary electrophoretic devices.

Abstract: Methods and compositions for modifying the surface of a polymeric substrate are provided. In the subject methods, at least a portion of the surface of the polymeric substrate is contacted with a biofouling resistant surfactant composition. At least a portion of the surfactant molecules of the composition are end modified with a non-reactive, charged end group that is charge stable over a pH range of about 2 to 12. The subject methods and compositions find particular use with electrophoretic devices, such as capillary electrophoretic devices.

Abstract: Methods for selectively separating at least one component from a multi-component fluidic sample are provided. In the subject methods, the fluidic sample is introduced into a micro-fluidic device that includes at least one micro-valve made up of a phase reversible material. The multi-component fluidic sample is then contacted with the microvalve in a microfluidic device under conditions sufficient for the at least one component to enter the microvalve, while the remaining constituents of the fluidic sample remain outside of the microvalve. Also provided are kits for use in practicing the subject methods, where the kits include at least a microfluidic device having a microvalve and instructional material (or means for obtaining the same) on how to use the device in the subject methods. The subject devices find use in a variety of applications, including sample desalting and concentration applications.

Abstract: Micro-fluid devices and methods for their use are provided. The subject devices are characterized by the presence of at least one micro-valve comprising a phase reversible material, e.g. a reversible gel, that reversibly changes its physical state in response to an applied stimulus, e.g. a thermoreversible gel. In using the subject device, fluid flow along a flow path of the device is modulated by applying an appropriate stimulus, e.g. changing the temperature, to the microvalve. The subject devices find use in a variety of applications, including micro-analytical applications.