Abstract: A biosensor includes a first base member and a parallel second base member disposed in a parallel and spaced relationship. At least one electrically conductive layer is deposited onto a facing surface of each of the first and second base members at respective first and second conductive regions. The first conductive region includes at least one layer made from a first electrically conductive material and the second conductive region includes at least one layer made from a second electrically conductive material, which is different from the first electrically conductive material. The first electrically conductive material is a precious metal with the first conductive regions of the biosensor defining cofacial electrodes and in which the second electrically conductive layer(s) is configured to electrically connect the cofacial electrodes to a test meter.

Abstract: An electrochemical-based analytical test strip (“EBAT”) for the determination of an analyte in a bodily fluid sample includes an electrically insulating substrate layer with a distal end and a patterned conductor layer that is disposed over the electrically-insulating substrate layer and has a working electrode (“WE”) and a counter/reference electrode (“C/RE”). The EBAT also includes a patterned insulation layer with an electrode exposure window configured to expose a WE exposed portion and a C/RE exposed portion, an enzymatic reagent layer; and a patterned spacer layer. The patterned insulation layer and the patterned spacer layer define a sample receiving chamber with a sample-receiving opening (“SRO”) at the distal end of the electrically insulating substrate layer and that extends across the WE exposed portion and the C/RE exposed portion. Furthermore, the enzymatic reagent layer is disposed over the WE and C/RE exposed portions and extends no more than 400 ?m toward the SRO.