Patents by Inventor Karol Lacki
Karol Lacki has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11285460Abstract: The invention discloses a separation matrix for purification of biological particles, comprising a plurality of particles having a porous core entity and a porous shell entity covering the core entity, wherein the core entity comprises at least 50 micromole/ml primary amines present on covalently attached ligands displaying at least two primary amines per ligand and the shell entity comprises less than 20 micromole/ml primary amines The invention further discloses a method of purifying biological particles and a method of manufacturing a separation matrix.Type: GrantFiled: February 18, 2015Date of Patent: March 29, 2022Assignee: CYTIVA BIOPROCESS R&D ABInventors: Jean-Luc Maloisel, Karolina Busson, Karol Lacki, Bjorn Noren, Helena Skoglar
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Publication number: 20210080434Abstract: A chromatography system with a main column comprising a chromatography resin, a first guard column and a second guard column, wherein the first guard column is connected to a first end of the main column, the second guard column is connected to a second end of the main column and the bed volumes of said first and second guard columns are each less than about 50% of the bed volume of the main column.Type: ApplicationFiled: November 24, 2020Publication date: March 18, 2021Inventor: Karol Lacki
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Publication number: 20200378934Abstract: There is provided a method and a corresponding system for determining binding capacities of a chromatography column. The method includes detecting a feed signal representative of the composition of a feed material provided to the inlet of the column. The method further includes detecting an effluent signal representative of the composition of the effluent from the column. The method further includes using the feed signal and the effluent signal to determine binding capacities of the column.Type: ApplicationFiled: August 18, 2020Publication date: December 3, 2020Inventors: Petra Bangtsson, Erik Estrada, Karol Lacki, Helena Skoglar
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Patent number: 10843104Abstract: A chromatography system for separation of a biopolymer is described, comprising at least one feed tank, at least one hold tank, at least one elution buffer tank, at least one eluate tank, at least two packed bed chromatography columns and at least one pump and at least one outlet detector both fluidically connected to said each packed bed chromatography column, wherein the feed tank, the hold tank(s), the elution buffer tank and the eluate tank are each fluidically connected to the packed bed chromatography columns via a system of valves.Type: GrantFiled: May 19, 2014Date of Patent: November 24, 2020Assignee: Cytiva Sweden ABInventors: Karol Lacki, Martin Hall
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Patent number: 10598638Abstract: A chromatography lab system comprising a cooling compartment arranged to hold both fraction collector devices and sample containers, whereby the cooling compartment comprises an identifying device which is arranged to identify the fraction collector devices such that fractions from the chromatography are collected only in the fraction collector devices.Type: GrantFiled: October 30, 2018Date of Patent: March 24, 2020Assignee: GE Healthcare Bio-Sciences ABInventors: Bjorn Olovsson, Karol Lacki, Mikael Berg
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Publication number: 20190128859Abstract: A chromatography lab system comprising a cooling compartment arranged to hold both fraction collector devices and sample containers, whereby the cooling compartment comprises an identifying device which is arranged to identify the fraction collector devices such that fractions from the chromatography are collected only in the fraction collector devices.Type: ApplicationFiled: October 30, 2018Publication date: May 2, 2019Applicant: GE Healthcare Bio-Sciences ABInventors: Bjorn Olovsson, Karol Lacki, Mikael Berg
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Patent number: 10265697Abstract: A micro titre plate comprising a number of wells filled with separation matrix. According to the invention the volume of the separation matrix is varied between at least some of the wells.Type: GrantFiled: October 12, 2016Date of Patent: April 23, 2019Inventor: Karol Lacki
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Publication number: 20180340917Abstract: A chromatography system with a main column comprising a chromatography resin, a first guard column and a second guard column, wherein the first guard column is connected to a first end of the main column, the second guard column is connected to a second end of the main column and the bed volumes of said first and second guard columns are each less than about 50% of the bed volume of the main column.Type: ApplicationFiled: August 6, 2018Publication date: November 29, 2018Applicant: GE Healthcare Bio-Sciences ABInventor: Karol Lacki
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Publication number: 20170056877Abstract: A micro titre plate comprising a number of wells filled with separation matrix. According to the invention the volume of the separation matrix is varied between at least some of the wells.Type: ApplicationFiled: October 12, 2016Publication date: March 2, 2017Inventor: Karol Lacki
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Patent number: 9551694Abstract: A micro titer plate comprising a number of wells filled with separation matrix. According to the invention the volume of the separation matrix is varied between at least some of the wells.Type: GrantFiled: October 7, 2009Date of Patent: January 24, 2017Assignee: GE Healthcare BioProcess R&D ABInventor: Karol Lacki
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Publication number: 20160367966Abstract: The invention discloses a separation matrix for purification of biological particles, comprising a plurality of particles having a porous core entity and a porous shell entity covering the core entity, wherein the core entity comprises at least 50 micromole/ml primary amines present on covalently attached ligands displaying at least two primary amines per ligand and the shell entity comprises less than 20 micromole/ml primary amines The invention further discloses a method of purifying biological particles and a method of manufacturing a separation matrix.Type: ApplicationFiled: February 18, 2015Publication date: December 22, 2016Inventors: Jean-Luc Maloisel, Karolina Busson, Karol Lacki, Bjorn Noren, Helena Skoglar
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Publication number: 20150328563Abstract: The invention discloses a process for chromatographic separation of at least one target biomolecule from at least one contaminant, comprising the steps of: a) providing an axial chromatography column comprising a consolidated bed of separation matrix particles, where the consolidated bed is confined between a bottom support net and a movable top adaptor; b) separating a target biomolecule from at least one contaminant on the column; c) raising the adaptor by at least 10% of the height of the consolidated bed; d) flowing a cleaning liquid upwards through the bed under conditions sufficient to liquefy the bed, and; e) repacking the matrix particles of the liquefied bed to create a consolidated bed and lowering the adaptor such that it contacts the packed bed and optionally compresses it, and; f) separating a target biomolecule from at least one contaminant on the column.Type: ApplicationFiled: December 12, 2013Publication date: November 19, 2015Applicant: GE Healthcare Bio-Sciences ABInventors: Karol Lacki, Hans J. Johansson
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Publication number: 20140251913Abstract: A chromatography system for separation of a biopolymer is described, comprising at least one feed tank, at least one hold tank, at least one elution buffer tank, at least one eluate tank, at least two packed bed chromatography columns and at least one pump and at least one outlet detector both fluidically connected to said each packed bed chromatography column, wherein the feed tank, the hold tank(s), the elution buffer tank and the eluate tank are each fluidically connected to the packed bed chromatography columns via a system of valves.Type: ApplicationFiled: May 19, 2014Publication date: September 11, 2014Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: KAROL LACKI, MARTIN HALL
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Publication number: 20130248451Abstract: A chromatography system for separation of a biopolymer is described, comprising at least one feed tank, at least one hold tank, at least one elution buffer tank, at least one eluate tank, at least two packed bed chromatography columns and for each packed bed chromatography column at least one pump and at least one outlet detector both connected to said each packed bed chromatography column, wherein the feed tank, the hold tank(s), the elution buffer tank and the eluate tank are each connected to the packed bed chromatography columns via a system of valves.Type: ApplicationFiled: December 2, 2011Publication date: September 26, 2013Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Martin Hall, Karol Lacki
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Publication number: 20130213884Abstract: A chromatography system with a main column comprising a chromatography resin, a first guard column and a second guard column, wherein the first guard column is connected to a first end of the main column, the second guard column is connected to a second end of the main column and the bed volumes of said first and second guard columns are each less than about 50% of the bed volume of the main column.Type: ApplicationFiled: October 24, 2011Publication date: August 22, 2013Applicant: GE HEALTHCARE BIO-SCIENCE ABInventor: Karol Lacki
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Patent number: 8268915Abstract: The present invention relates to a liquid mixture comprising a first polymer, which is a poly(acid), a second polymer, which is a poly(ether), and at least one salt, wherein the molecular weight of the poly(acid) is in the range of 1000-100,000 Da. The second polymer is selected to be capable of forming immiscible aqueous phases in the presence of the poly(acid) and salt. The poly(acid) may be selected from the group consisting of poly(acrylic acid) and poly(methacrylic acid), and the second synthetic polymer may comprise ethylene oxide. The invention may be used for separation of biomolecules, cells or particles.Type: GrantFiled: June 16, 2008Date of Patent: September 18, 2012Assignee: GE Healthcare Bio-Sciences ABInventors: Hans O. Johansson, James Van Alstine, Rolf Hjorth, Karol Lacki, Emmanuel Macedo, Gunnar Malmquist, Jamil Shanagar
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Publication number: 20120091063Abstract: A method for determining binding capacities of a chromatography column (1; 39, 47, 59; 107, 109, 111, 113), comprising: detecting a feed signal (21; 201) representative of the composition of a feed material provided to the inlet of the column; detecting an effluent signal (23; 203, 205, 207, 209) representative of the composition of the effluent from the column; using the feed signal and the effluent signal to determine binding capacities of the column. And a method for controlling a chromatography system comprising at least one column, comprising the steps of: determining binding capacities of the at least one chromatography column according to above; and controlling the start and stop of the different chromatography process steps according to the determined binding capacities.Type: ApplicationFiled: June 21, 2010Publication date: April 19, 2012Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Petra Bangtsson, Erik Estrada, Karol Lacki, Helena Skoglar
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Publication number: 20120022239Abstract: The present invention relates to methods of isolating biomolecules. More particularly, the invention relates to methods for isolating antibodies (mAbs) and related proteins including antibody fragments (Fabs) under conditions where they are positive and relatively hydrophobic and will react with negatively charged polymer to form polymer-protein complexes which precipitate. The isolation can be accomplished using inexpensive and biocompatible negatively charged polymers such as polyacrylic acid or carboxymethyldextran polymers of various molecular weights as precipitant. It occurs at relatively high concentrations of polymer (e.g. 10%) and high salt concentration (>50 mM) and conductivity (e.g. >10 mS/cm) over wide range of pH.Type: ApplicationFiled: January 11, 2010Publication date: January 26, 2012Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: James Van Alstine, Jamil Shanagar, Rolf Hjorth, Karol Lacki
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Publication number: 20110257378Abstract: The invention relates to an aqueous two phase extraction (ATPE) augmented precipitation process, which may be used to recover and also partially purify therapeutic proteins, including monoclonal antibodies from a crude multi-component mixture. The process involves the formation of a forward extraction PEG-Phosphate ATPE system in which the target product is preferentially partitioned to the polymer rich phase. A second ATPE back extraction system is then formed by introducing the polymer rich phase from the forward extraction to a new phosphate salt rich phase, causing the product to precipitate at the interface between the two phases. This precipitate is then recovered and resolubilised in a suitable buffer and may be passed on for further purification.Type: ApplicationFiled: November 18, 2009Publication date: October 20, 2011Applicant: GE HEALTHCARE BIO-SCIENCES ABInventors: Richard Tran, Nigel J. Titchener-Hooker, Karol Lacki
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Publication number: 20110195517Abstract: A micro titre plate comprising a number of wells filled with separation matrix. According to the invention the volume of the separation matrix is varied between at least some of the wells.Type: ApplicationFiled: October 7, 2009Publication date: August 11, 2011Applicant: GE Healthcare Bio-Science ABInventor: Karol Lacki