Patents by Inventor Karolien Van Huynegem
Karolien Van Huynegem has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220340621Abstract: Microorganisms are provided, such as lactic acid bacteria (e.g., Lactococcus lactis) containing an exogenous nucleic acid encoding an IL-10 polypeptide and an exogenous nucleic acid encoding a CeD-specific antigen (e.g., a gliadin polypeptide comprising at least one HLA-DQ2 specific epitope, at least one deamidated HLA-DQ2 specific epitope, at least one HLA-DQ8 specific epitope, at least one deamidated HLA-DQ8 specific epitope, or a combination of (a) at least one HLA-DQ2-specific epitope and/or at least one deamidated HLA-DQ2 specific epitope, and (b) at least one HLA-DQ8 specific epitope and/or at least one deamidated HLA-DQ8 specific epitope) polypeptide, wherein both exogenous nucleic acids are integrated into the bacterial chromosome. Such microbial strains are suitable for human therapy. Compositions (e.g., pharmaceutical compositions), methods of using the microorganisms and compositions are provided, e.g., for the treatment of celiac disease (CeD).Type: ApplicationFiled: September 25, 2020Publication date: October 27, 2022Applicant: INTREXON ACTOBIOTICS NV d/b/a Precigen ActobioInventors: Lothar STEIDLER, Karolien VAN HUYNEGEM
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Publication number: 20210163961Abstract: The invention relates to polycistronic expression in gram-positive bacterium and in particular concerns polycistronic expression units comprising one or more gene endogenous to the gram-positive bacterium transcriptionally coupled to one or more genes exogenous to the bacterium.Type: ApplicationFiled: December 4, 2020Publication date: June 3, 2021Applicant: INTREXON ACTOBIOTICS NVInventors: Klaas VANDENBROUCKE, Karolien VAN HUYNEGEM, Lothar STEIDLER
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Patent number: 10988770Abstract: The invention relates to polycistronic expression in gram-positive bacterium and in particular concerns polycistronic expression units comprising one or more gene endogenous to the gram-positive bacterium transcriptionally coupled to one or more genes exogenous to the bacterium.Type: GrantFiled: February 15, 2018Date of Patent: April 27, 2021Assignee: INTREXON ACTOBIOTICS NVInventors: Klaas Vandenbroucke, Karolien Van Huynegem, Lothar Steidler
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Patent number: 10793825Abstract: The present invention relates to gram positive bacteria with increased stress resistance and/or improved storage characteristics. In particular, the invention relates to gram positive bacterium which accumulate intracellular trehalose. The gram positive bacterium according to the invention lack cellobiose-specific PTS system IIC component (PtcC) activity. The gram positive bacterium may further lack trehalose 6-phosphate phosphorylase (TrePP) activity. The gram positive bacterium may further overexpress trehalose transporters. The invention further relates to compositions comprising such gram positive bacterium as well as methods and uses thereof.Type: GrantFiled: December 13, 2019Date of Patent: October 6, 2020Assignee: INTREXON ACTOBIOTICS NVInventors: Lothar Steidler, Karolien Van Huynegem, Klaas Vandenbroucke
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Publication number: 20200165564Abstract: The present invention relates to gram positive bacteria with increased stress resistance and/or improved storage characteristics. In particular, the invention relates to gram positive bacterium which accumulate intracellular trehalose. The gram positive bacterium according to the invention lack cellobiose-specific PTS system IIC component (PtcC) activity. The gram positive bacterium may further lack trehalose 6-phosphate phosphorylase (TrePP) activity. The gram positive bacterium may further overexpress trehalose transporters. The invention further relates to compositions comprising such gram positive bacterium as well as methods and uses thereof.Type: ApplicationFiled: December 13, 2019Publication date: May 28, 2020Applicant: INTREXON ACTOBIOTICS NVInventors: Lothar Steidler, Karolien Van Huynegem, Klaas Vandenbroucke
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Patent number: 10519418Abstract: The present invention relates to gram positive bacteria with increased stress resistance and/or improved storage characteristics. In particular, the invention relates to gram positive bacterium which accumulate intracellular trehalose. The gram positive bacterium according to the invention lack cellobiose-specific PTS system IIC component (PtcC)activity. The gram positive bacterium may further lack trehalose 6-phosphate phosphorylase (TrePP)activity. The gram positive bacterium may further overexpress trehalose transporters. The invention further relates to compositions comprising such gram positive bacterium as well as methods and uses thereof.Type: GrantFiled: April 23, 2018Date of Patent: December 31, 2019Assignee: INTREXON ACTOBIOTICS NVInventors: Lothar Steidler, Karolien Van Huynegem, Klaas Vandenbroucke
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Publication number: 20180237739Abstract: The present invention relates to gram positive bacteria with increased stress resistance and/or improved storage characteristics. In particular, the invention relates to gram positive bacterium which accumulate intracellular trehalose. The gram positive bacterium according to the invention lack cellobiose-specific PTS system IIC component (PtcC)activity. The gram positive bacterium may further lack trehalose 6-phosphate phosphorylase (TrePP)activity. The gram positive bacterium may further overexpress trehalose transporters. The invention further relates to compositions comprising such gram positive bacterium as well as methods and uses thereof.Type: ApplicationFiled: April 23, 2018Publication date: August 23, 2018Applicant: INTREXON ACTOBIOTICS NVInventors: Lothar Steidler, Karolien Van Huynegem, Klaas Vandenbroucke
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Patent number: 10030234Abstract: The present invention relates to a gram positive bacterium, preferably a lactic acid bacterium (LAB) or Bifidobacterium, with increased stress resistance and/or improved manufacturing, processing and/or storage characteristics. In particular, the invention relates to a gram positive bacterium which accumulate intracellular trehalose. The gram positive bacterium according to the invention lack trehalose 6-phosphate phosphorylase (TrePP) activity. The gram positive bacterium may further lack cellobiose-specific PTS system IIC component (ptcC) activity. The gram positive bacterium may further overexpress trehalose transporters. The invention further relates to compositions comprising such gram positive bacterium as well as methods and uses thereof.Type: GrantFiled: September 7, 2016Date of Patent: July 24, 2018Assignee: INTREXON ACTOBIOTICS NVInventors: Lothar Steidler, Karolien Van Huynegem, Klaas Vandenbroucke
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Publication number: 20180187203Abstract: The invention relates to polycistronic expression in gram-positive bacterium and in particular concerns polycistronic expression units comprising one or more gene endogenous to the gram-positive bacterium transcriptionally coupled to one or more genes exogenous to the bacterium.Type: ApplicationFiled: February 15, 2018Publication date: July 5, 2018Applicant: INTREXON ACTOBIOTICS NVInventors: Klaas VANDENBROUCKE, Karolien VAN HUYNEGEM, Lothar STEIDLER
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Patent number: 9982228Abstract: The present invention relates to gram positive bacteria with increased stress resistance and/or improved storage characteristics. In particular, the invention relates to gram positive bacterium which accumulate intracellular trehalose. The gram positive bacterium according to the invention lack cellobiose-specific PTS system IIC component (PtcC) activity. The gram positive bacterium may further lack trehalose 6-phosphate phosphorylase (TrePP) activity. The gram positive bacterium may further overexpress trehalose transporters. The invention further relates to compositions comprising such gram positive bacterium as well as methods and uses thereof.Type: GrantFiled: January 21, 2016Date of Patent: May 29, 2018Assignee: INTREXON ACTOBIOTICS NVInventors: Lothar Steidler, Karolien Van Huynegem, Klaas Vandenbroucke
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Patent number: 9920324Abstract: The invention relates to polycistronic expression in gram-positive bacterium and in particular concerns polycistronic expression units comprising one or more gene endogenous to the gram-positive bacterium transcriptionally coupled to one or more genes exogenous to the bacterium.Type: GrantFiled: June 1, 2012Date of Patent: March 20, 2018Assignee: INTREXON ACTOBIOTICS NVInventors: Klaas Vanden-Broucke, Karolien Van Huynegem, Lothar Steidler
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Publication number: 20160376574Abstract: The present invention relates to a gram positive bacterium, preferably a lactic acid bacterium (LAB) or Bifidobacterium, with increased stress resistance and/or improved manufacturing, processing and/or storage characteristics. In particular, the invention relates to a gram positive bacterium which accumulate intracellular trehalose. The gram positive bacterium according to the invention lack trehalose 6-phosphate phosphorylase (TrePP) activity. The gram positive bacterium may further lack cellobiose-specific PTS system IIC component (ptcC) activity. The gram positive bacterium may further overexpress trehalose transporters. The invention further relates to compositions comprising such gram positive bacterium as well as methods and uses thereof.Type: ApplicationFiled: September 7, 2016Publication date: December 29, 2016Inventors: Lothar Steidler, Karolien Van Huynegem, Klaas Vandenbroucke
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Patent number: 9458467Abstract: The present invention relates to a gram positive bacterium, preferably a lactic acid bacterium (LAB) or Bifidobacterium, with increased stress resistance and/or improved manufacturing, processing and/or storage characteristics. In particular, the invention relates to a gram positive bacterium which accumulate intracellular trehalose. The gram positive bacterium according to the invention lack trehalose 6-phosphate phosphorylase (TrePP) activity. The gram positive bacterium may further lack cellobiose-specific PTS system IIC component (ptcC) activity. The gram positive bacterium may further overexpress trehalose transporters. The invention further relates to compositions comprising such gram positive bacterium as well as methods and uses thereof.Type: GrantFiled: September 21, 2012Date of Patent: October 4, 2016Assignee: INTREXON ACTOBIOTICS NVInventors: Lothar Steidler, Karolien Van Huynegem, Klaas Vandenbroucke
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Patent number: 9347036Abstract: The present invention relates to gram positive bacteria with increased stress resistance and/or improved storage characteristics. In particular, the invention relates to gram positive bacterium which accumulate intracellular trehalose. The gram positive bacterium according to the invention lack cellobiose-specific PTS system IIC component (PtcC) activity. The gram positive bacterium may further lack trehalose 6-phosphate phosphorylase (TrePP) activity. The gram positive bacterium may further overexpress trehalose transporters. The invention further relates to compositions comprising such gram positive bacterium as well as methods and uses thereof.Type: GrantFiled: September 21, 2012Date of Patent: May 24, 2016Assignee: INTREXON ACTOBIOTICS NVInventors: Lothar Steidler, Karolien Van Huynegem, Klaas Vandenbroucke
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Publication number: 20140234371Abstract: The present invention relates to a gram positive bacterium, preferably a lactic acid bacterium (LAB) or Bifidobacterium, with increased stress resistance and/or improved manufacturing, processing and/or storage characteristics. In particular, the invention relates to a gram positive bacterium which accumulate intracellular trehalose. The gram positive bacterium according to the invention lack trehalose 6-phosphate phosphorylase (TrePP) activity. The gram positive bacterium may further lack cellobiose-specific PTS system IIC component (ptcC) activity. The gram positive bacterium may further overexpress trehalose transporters. The invention further relates to compositions comprising such gram positive bacterium as well as methods and uses thereof.Type: ApplicationFiled: September 21, 2012Publication date: August 21, 2014Inventors: Lothar Steidler, Karolien Van Huynegem, Klaas Vandenbroucke
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Publication number: 20140227229Abstract: The present invention relates to gram positive bacteria with increased stress resistance and/or improved storage characteristics. In particular, the invention relates to gram positive bacterium which accumulate intracellular trehalose. The gram positive bacterium according to the invention lack cellobiose-specific PTS system IIC component (PtcC) activity. The gram positive bacterium may further lack trehalose 6-phosphate phosphorylase (TrePP) activity. The gram positive bacterium may further overexpress trehalose transporters. The invention further relates to compositions comprising such gram positive bacterium as well as methods and uses thereof.Type: ApplicationFiled: September 21, 2012Publication date: August 14, 2014Inventors: Lothar Steidler, Karolien Van Huynegem, Klaas Vandenbroucke
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Publication number: 20140105863Abstract: The invention relates to polycistronic expression in gram-positive bacterium and in particular concerns polycistronic expression units comprising one or more gene endogenous to the gram-positive bacterium transcriptionally coupled to one or more genes exogenous to the bacterium.Type: ApplicationFiled: June 1, 2012Publication date: April 17, 2014Applicant: ACTOGENIX N.V.Inventors: Klaas Vanden-Broucke, Karolien Van Huynegem, Lothar Steidler