Abstract: The present invention relates to a method for the isolation and/or identification of known or unknown sequences of nucleic acids (target sequences) optionally marked with reporter groups by base specific hybridation with complementary sequences using nucleolipids. The nucleolipids are prepared by lipophilizing nucleosides of formula (Ia) wherein Q represents a group having a substituted tetrahydrofuran ring and Bas represents a group having one or more heterocyclic rings having one or more heterocyclic nitrogen atoms.

Abstract: The present invention relates to a method for the isolation and/or identification of known or unknown sequences of nucleic acids (target sequences) optionally marked with reporter groups by base specific hybridation with, essentially, complementary sequences (in the following referred to as sample oligo-nucleotides, sample sequences or sample nucleic acids), which belong to a library of sequences. Further, the invention relates to nucleolipids used in the method of the invention and a process for the preparation of said nucleolipids. In addition, the invention refers to a pharmaceutical composition comprising said nucleolipids.

Abstract: The present invention relates to a method for detecting an analyte in a sample comprising the steps of providing detection probes being labeled with a first reporter, which detection probes are capable of binding to the analyte, providing a solid support, providing capture probes being bound or capable of binding to the solid support, which capture probes are capable of binding to the analyte, thus concentrating the analyte on the solid support, contacting the sample with the detection probes, the solid support and the capture probes, and detecting the detection probes, wherein the detection of detection probes is conducted in the presence of quenching probes binding to surplus detection probes not being bound to the analyte and thereby quenching at least partially an emission of the first reporter of said surplus detection probes and/or the solid support is labeled with a second reporter different from the first reporter, imaging the sample at an emission wavelength of the second reporter, generating a m

Abstract: The invention relates to a measuring device for measuring at least one property of cells or vesicles, characterized in that is divided into two chambers by a hydrophobic partition having a horizontal opening, these chambers containing electrolyte solution and said opening being closed by a lipid double layer. The invention is characterized in that at least one cell or vesicle contacts the lipid double layer. The invention also relates to methods involving the use of the measuring device and to arrays, measuring chambers and microtitration plates all suited therefor.

Abstract: The present invention relates to a method for detecting an analyte in a sample comprising the steps of providing detection probes being labeled with a first reporter, which detection probes are capable of binding to the analyte, providing a solid support, providing capture probes being bound or capable of binding to the solid support, which capture probes are capable of binding to the analyte, thus concentrating the analyte on the solid support, contacting the sample with the detection probes, the solid support and the capture probes, and detecting the detection probes, wherein the detection of detection probes is conducted in the presence of quenching probes binding to surplus detection probes not being bound to the analyte and thereby quenching at least partially an emission of the first reporter of said surplus detection probes and/or the solid support is labeled with a second reporter different from the first reporter, imaging the sample at an emission wavelength of the second reporter, generating a m

Abstract: The present invention relates to a method for detecting analytes in a sample comprising the steps of providing a solid support, providing capture probes being bound or capable of binding to the solid support, which capture probes are also capable of binding to the analytes, thus concentrating the analytes on the solid support, providing detection probes which are capable of binding to the analytes, contacting the sample with the detection probes, the solid support and the capture probes, and detecting through use of confocal observation the analytes which are bound to the detection probes.

Abstract: The present invention relates to a method for detecting analytes in a sample comprising the steps of providing a solid support, providing capture probes being bound or capable of binding to the solid support, which capture probes are also capable of binding to the analytes, thus concentrating the analytes on the solid support, providing detection probes which are capable of binding to the analytes, contacting the sample with the detection probes, the solid support and the capture probes, and detecting through use of confocal observation the analytes which are bound to the detection probes.

Abstract: The invention relates to a measuring device for measuring at least one property of cells or vesicles, characterized in that is divided into two chambers by a hydrophobic partition having a horizontal opening, these chambers containing electrolyte solution and said opening being closed by a lipid double layer. The invention is characterized in that at least one cell or vesicle contacts the lipid double layer. The invention also relates to methods involving the use of the measuring device and to arrays, measuring chambers and microtitration plates all suited therefor.

Abstract: A device for the measurement of chemical and/or biological samples, in particular by means of luminescence spectroscopy, comprises an irradiation unit, a sample receiver, at least one optical unit and a detector unit. The color marker in the sample, which contains at least one color marker, is stimulated by irradiation into producing luminescence and gives off light. The light emitted by the color markers is detected by detectors in the detector unit. According to the invention, the measurement results may be improved by the irradiation unit generating pulsed irradiation. The irradiation unit is thus preferably controlled by a control unit in such a way that the irradiation pulses impinge on the sample in a temporal sequence.

Abstract: A method for distinguishing samples having flourescent particles includes (a) monitoring intensity fluctuations of fluorescence emitted by the particles in at least one measurement volume by detecting sequences of photon counts by at least one photon detector, (b) determining, from the sequences of photon counts, intermediate statistical data involving at least two probability functions of the number of photon counts detected in different sets of counting time intervals, and (c) determining from the intermediate statistical data a distribution of particles as a function of at least two arguments, wherein one argument is a specific brightness of the particles, or a measure thereof, and another argument is a diffusion coefficient of the particles, or a measure thereof.

Abstract: A device for the measurement of chemical and/or biological samples, in particular by means of luminescence spectroscopy, comprises an irradiation unit (12), a sample receiver (10), at least one optical unit (30) and a detector unit (40). Electromagnetic radiation of various wavelength ranges and/or polarizations are led from the sample (10) from the irradiation unit (12). The color marker in the sample (10), which contains at least one color marker, is stimulated into producing luminescence and gives off light. The emitted light is led by means of an optical unit to a detector unit. The light emitted by the color markers is detected by detectors (42, 44, 46, 48) in the detector unit. According to the invention, the measurement results may be improved on the irradiation unit (12) generating a pulsed irradiation. The irradiation unit is thus preferably controlled by a control unit (18) in such a way that the irradiation pulses impinge on the sample (10) in a temporal sequence.

Abstract: Paint buckets and other sales packaging for paints, solvents and the like must be cleaned from contamination by residual amounts of the previous content before a reuse. A container according to the invention has protective layers which are applied to its container walls in several layers and which, starting from a detaching point, can be pulled off together with adhering contaminations so that the container can be reused again without any cleaning expenditures.