Patents by Inventor Karsten Kragh
Karsten Kragh has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20080107773Abstract: We disclose a food additive comprising a PS4 variant polypeptide, in which the PS4 variant polypeptide is derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises substitutions at the following positions: 6121D, 134, 141, 157, 223, 307 and 334 with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1.Type: ApplicationFiled: September 19, 2007Publication date: May 8, 2008Inventors: Karsten Kragh, Bo Sorensen
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Publication number: 20070141693Abstract: The invention provides for a PS4 variant polypeptide derivable from a parent polypeptide having amylase activity which may be selected from the group consisting of: (a) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 161, 178, 179, 223, 229, 272, 303, 307, 309 and 334; (b) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 145, 146, 157, 178, 179, 223, 229, 272, 303, 307 and 334; (c) a polypeptide comprising an amino acid mutation at each of positions 33, 34, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; and (d) a polypeptide comprising an amino acid mutation at each of positions 3, 33, 34, 70, 121, 134, 141, 146, 157, 178, 179, 223, 229, 272, 303, 307, 309 and 334; with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1, uses of such a polypeptide as a food or feed additive, and nucleic acids encoding such.Type: ApplicationFiled: July 7, 2006Publication date: June 21, 2007Inventors: Casper Berg, Patrick Maria Derkx, Carol Fioresi, Gijsbert Gerritse, Anja Kellet-Smith, Karsten Kragh, Wei Liu, Andrew Shaw, Bo Sorensen, Charlotte Thoudahl
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Publication number: 20070072270Abstract: We disclose a PS4 variant polypeptide derivable from a parent polypeptide, the parent polypeptide having non-maltogenic exoamylase activity, which PS4 variant polypeptide comprises one or more of the following substitutions: G69P, A141P, G223A, A268P, G313P, S399P and G400P, with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. Such PS4 variant polypeptides may be used as exo-amylases, particularly as non-maltogenic exoamylases. Combinations of such PS4 variant polypeptides together with Novamyl are disclosed.Type: ApplicationFiled: December 8, 2006Publication date: March 29, 2007Applicant: Danisco A/SInventors: Karsten Kragh, Harm Mulder, Steffen Petersen, Helle Fomsgaard, Oene Veltman
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Publication number: 20070020731Abstract: This is invention relates to amylase polypeptides, and nucleic acids encoding the polpypeptides and uses thereof. The amylases of the present invention have been engineered to have more beneficial qualities. Specifically, the amylases of the current invention show an altered thermostability.Type: ApplicationFiled: September 27, 2006Publication date: January 25, 2007Inventors: Karsten Kragh, Casper Berg, Patrick Derkx, Anja Kellett-Smith, Charlotte Thoudahl, Oene Veltman, Carol Fioresi, Gijsbert Gerritse, Wei Liu, Andrew Shaw
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Publication number: 20070020727Abstract: This invention relates to amylase polypeptides, and nucleic acids encoding the polpypeptides and uses thereof. The amylases of the present invention have been engineered to have more beneficial qualities. Specifically, the amylases of the current invention show an altered exospecifity.Type: ApplicationFiled: September 19, 2006Publication date: January 25, 2007Inventors: Casper Berg, Kirsten Bojsen, Patrick Derkx, Carol Fioresi, Gijsbert Gerritse, Karsten Kragh, Wei Liu, Andrew Shaw, Charlotte Thoudahl, Anja Kellett-Smith
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Publication number: 20060134266Abstract: An enzyme is described which enzyme is derived from family 13 of ?-amylases. The enzyme variant is obtainable by modifying a CGTase or a maltogenic ?-amylase. The enzyme is useful in preparing a food or a food product such as bakery products.Type: ApplicationFiled: September 12, 2005Publication date: June 22, 2006Inventors: Karsten Kragh, Hans Leemhuis, Lubbert Dijkhuizen, Bauke Dijkstra
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Publication number: 20060073583Abstract: We describe a food additive comprising a PS4 variant polypeptide, in which the PS4 variant polypeptide is derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises an amino acid mutation at one or more positions selected from the group consisting of: 146, 157, 158, 198, 229, 303, 306, 309, 316 and 353, with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. Preferably, the PS4 variant polypeptide comprises an amino acid mutation selected from the group consisting of: 146G, 146M, 157M, 158T, 158A, 158S, 198W, 198F, 229P, 303E, 303D, 306T, 306G, 309P, 316S, 316P, 316K, 316Q and 353T, preferably 146G, 157M, 158T, 198W, 229P, 303E, 303D, 306T, 306G, 309P, 316S, 316P or 353T. in highly preferred embodiments, the PS4 variant polypeptide has a sequence selected from SEQ ID NO: 15 to 28.Type: ApplicationFiled: September 22, 2004Publication date: April 6, 2006Inventors: Karsten Kragh, Bo Sorensen
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Publication number: 20060018997Abstract: We describe a food additive comprising a PS4 variant polypeptide, in which the PS4 variant polypeptide is derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises an amino acid substitution at position 161 with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. The PS4 variant polypeptide may further comprise one or more further mutations at a position selected from the group consisting of: 121 and 223, preferably 121F, 121Y, 121W, 223E and 223K, more preferably G121F, G121Y, G121W, G223E and/or G223K, or the group consisting of: 134, 141, 157, 223, 307, 334, 33 and 34, preferably G134R, A141P, I157L, G223A, H307L, S334P, N33Y and D34N. In preferred embodiments, the PS4 variant polypeptide further comprises a mutation at position 87, preferably G87S, a mutation at position 178, preferably L178F, and/or a mutation at position 179, preferably A179T.Type: ApplicationFiled: July 7, 2004Publication date: January 26, 2006Inventors: Karsten Kragh, Bo Sorensen
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Publication number: 20060008890Abstract: We describe a food additive comprising a PS4 variant polypeptide, in which the PS4 variant polypeptide is derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises an amino acid substitution at position 121 with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. The PS4 variant polypeptide may further comprise one or more further mutations at a position selected from the group consisting of: 161 and 223, preferably 161A, 223E and 223K, more preferably S161A, G223E and/or G223K, or the group consisting of: 134, 141, 157, 223, 307, 334, 33 and 34, preferably G134R, A141P, I157L, G223A, H307L, S334P, N33Y and D34N. In preferred embodiments, the PS4 variant polypeptide further comprises a mutation at position 87, preferably G87S, a mutation at position 178, preferably L178F, and/or a mutation at position 179, preferably A179T.Type: ApplicationFiled: July 7, 2004Publication date: January 12, 2006Inventors: Karsten Kragh, Bo Sorensen
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Publication number: 20060008888Abstract: We describe a food additive comprising a PS4 variant polypeptide, in which the PS4 variant polypeptide is derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises an amino acid substitution at position 223 with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. The PS4 variant polypeptide may further comprise one or more further mutations at a position selected from the group consisting of: 121 and 161, preferably 121F, 121Y, 121W and 161A, more preferably G121F, G121Y, G121W and/or S161A, or the group consisting of: 134, 141, 157, 223, 307, 334, 33 and 34, preferably G134R, A141P, I157L, G223A, H307L, S334P, N33Y and D34N. In preferred embodiments, the PS4 variant polypeptide further comprises a mutation at position 87, preferably G87S, a mutation at position 178, preferably L178F, and/or a mutation at position 179, preferably A179T.Type: ApplicationFiled: July 7, 2004Publication date: January 12, 2006Inventors: Karsten Kragh, Bo Sorensen
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Publication number: 20050136524Abstract: This invention relates to amylase polypeptides, and nucleic acids encoding the polpypeptides and uses thereof. The amylases of the present invention have been engineered to have more beneficial qualities. Specifically, the amylases of the current invention show an altered exospecifity.Type: ApplicationFiled: July 7, 2004Publication date: June 23, 2005Inventors: Karsten Kragh, Kirsten Bojsen, Casper Berg, Patrick Derkx, Anja Kellett-Smith, Charlotte Thoudahl, Carol Fioresi, Wei Liu, Andrew Shaw, Gijsbert Gerritse
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Publication number: 20050137111Abstract: This invention relates to amylase polypeptides, and nucleic acids encoding the polpypeptides and uses thereof. The amylases of the present invention have been engineered to have more beneficial qualities. Specifically, the amylases of the current invention show an altered thermostability.Type: ApplicationFiled: July 7, 2004Publication date: June 23, 2005Inventors: Karsten Kragh, Casper Berg, Patrick Derkx, Anja Kellett-Smith, Charlotte Thoudahl, Oene Veltman, Carol Fioresi, Wei Liu, Andrew Shaw, Gijsbert Gerritse
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Publication number: 20050112237Abstract: We disclose a food additive comprising a PS4 variant polypeptide, in which the PS4 variant polypeptide is derivable from a parent polypeptide having non-maltogenic exoamylase activity, in which the PS4 variant polypeptide comprises substitutions at the following positions: G121D, 134, 141, 157, 223, 307 and 334 with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1.Type: ApplicationFiled: July 7, 2004Publication date: May 26, 2005Inventors: Karsten Kragh, Bo Sorensen
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Publication number: 20050037053Abstract: The present invention relates to a component comprising an enzyme for use in a feed comprising strach: wherein the enzyme has amylase activity and is capable of degrading resistant starch.Type: ApplicationFiled: December 13, 2002Publication date: February 17, 2005Inventors: Mai Isaksen, Karsten Kragh, Troels Gravesen
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Publication number: 20050037391Abstract: We disclose a PS4 variant polypeptide derivable from a parent polypeptide, the parent polypeptide having non-maltogenic exoamylase activity, which PS4 variant polypeptide comprises one or more of the following substitutions: G69P, A141P, G223A, A268P, G313P, S399P and G400P, with reference to the position numbering of a Pseudomonas saccharophilia exoamylase sequence shown as SEQ ID NO: 1. Such PS4 variant polypeptides may be used as exo-amylases, particularly as non-maltogenic exoamylases. Combinations of such PS4 variant polypeptides together with Novamyl are disclosed.Type: ApplicationFiled: June 10, 2004Publication date: February 17, 2005Inventors: Karsten Kragh, Harm Mulder, Steffan Petersen, Helle Fomsgaard, Oene Robert Veltman
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Publication number: 20030207409Abstract: A method of preparing the sugar 1,5-D-anhydrofructose is described. The method comprises treating an &agr;-1,4-glucan with an &agr;-1,4-glucan lyase wherein the enzyme is used in substantially pure form. In a preferred embodiment, if the glucan contains links other than and in addition to the &agr;-1,4-links, the &agr;-1,4-glucan lyase is used in conjunction with a suitable reagent that can break the other links.Type: ApplicationFiled: May 30, 2003Publication date: November 6, 2003Applicant: DANISCO A/SInventors: Shukun Yu, Kirsten Bojsen, Karsten Kragh, Maja Bojko, John Nielsen, Jan Marcussen, Tove Christensen
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Patent number: 6632643Abstract: A method of preparing the sugar 1,5-D-anhydrofructose is described. The method comprises treating an &agr;-1,4-glucan with an &agr;-1,4-glucan lyase wherein the enzyme is used in substantially pure form. In a preferred embodiment, if the glucan contains links other than and in addition to the &agr;-1,4-links, the &agr;-1,4-glucan lyase is used in conjunction with a suitable reagent that can break the other links.Type: GrantFiled: March 29, 1999Date of Patent: October 14, 2003Assignee: Danisco A/SInventors: Shukun Yu, Kirsten Bojsen, Karsten Kragh, Maja Bojko, John Nielsen, Jan Marcussen, Tove Christensen
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Publication number: 20020142403Abstract: A method of preparing the sugar 1,5-D-anhydrofructose is described. The method comprises treating an alpha -1,4-glucan with an alpha -1,4-glucan lyase wherein the enzyme is used in substantially pure form. In a preferred embodiment, if the glucan contains links other than and in addition to the alpha -1,4-links, the alpha -1,4-glucan lyase is used in conjunction with a suitable reagent that can break the other links.Type: ApplicationFiled: March 29, 1999Publication date: October 3, 2002Inventors: SHUKUN YU, KIRSTEN BOJSEN, KARSTEN KRAGH, MAJA BOJKO, JOHN NIELSON, JAN MARCUSSEN, TOVE CHRISTENSEN
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Patent number: 6013504Abstract: A method of preparing .alpha.-1,4-glucan lyase enzymes is described. The method comprises isolating the enzymes from a fungally infected algae. The amino acid sequences of the enzymes have been determined. The nucleic acid sequences coding for the enzymes have also been determined.Type: GrantFiled: July 2, 1996Date of Patent: January 11, 2000Assignee: Danisco A/SInventors: Shukun Yu, Kirsten Bojsen, Karsten Kragh, Maja Bojko, John Nielsen, Jan Marcussen
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Patent number: 5908760Abstract: A method of preparing .varies.-glucan lyase enzymes is described. The method comprises isolating the enzymes from a culture of fungus wherein the culture is substantially free of any other organisms. Also described are the amino acid sequences for the enzymes and their coding sequences.Type: GrantFiled: July 8, 1996Date of Patent: June 1, 1999Assignee: Danisco A/SInventors: Kirsten Bojsen, Shukun Yu, Karsten Kragh, Tove Christensen, Jan Marcussen