Patents by Inventor Karsten Temme

Karsten Temme has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20240010576
    Abstract: Disclosed herein are methods of increasing nitrogen fixation in a non-leguminous plant. The methods can comprise exposing the plant to a plurality of bacteria. Each member of the plurality comprises one or more genetic variations introduced into one or more genes or non-coding polynucleotides of the bacteria's nitrogen fixation or assimilation genetic regulatory network, such that the bacteria are capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen. The bacteria are not intergeneric microorganisms. Additionally, the bacteria, in planta, produce 1% or more of the fixed nitrogen in the plant.
    Type: Application
    Filed: June 30, 2023
    Publication date: January 11, 2024
    Applicant: Pivot Bio, Inc.
    Inventors: Karsten Temme, Alvin Tamsir, Sarah Bloch, Rosemary Clark, Emily Tung
  • Patent number: 11810646
    Abstract: Methods for design of genetic circuits are provided.
    Type: Grant
    Filed: February 13, 2020
    Date of Patent: November 7, 2023
    Assignee: The Regents of the University of California
    Inventors: Chunbo Lou, Tae Seok Moon, Virgil Rhodius, Brynne Stanton, Alvin Tasmir, Karsten Temme, Chris Voigt
  • Publication number: 20230276807
    Abstract: Abstract: A method for reducing variation in whole plant nitrogen includes providing, to a locus,a plurality of crop plants and a plurality of nitrogen fixing microbes that colonize the rhizosphere of said plurality of crop plants and supply the plants with fixed N. The variation in whole plant nitrogen of the plurality of crop plants colonized by said nitrogen fixing microbes, at a given growth stage and as measured across the locus, is lower than a variation in whole plant nitrogen of a control plurality of crop plants, when the control plurality of crop plants is provided to the locus.
    Type: Application
    Filed: August 4, 2021
    Publication date: September 7, 2023
    Applicant: Pivot Bio, Inc.
    Inventors: Mark REISINGER, Ernest SANDERS, Karsten TEMME, David BROWN, Stephen FICK
  • Patent number: 11739032
    Abstract: Disclosed herein are methods of increasing nitrogen fixation in a non-leguminous plant. The methods can comprise exposing the plant to a plurality of bacteria. Each member of the plurality comprises one or more genetic variations introduced into one or more genes or non-coding polynucleotides of the bacteria's nitrogen fixation or assimilation genetic regulatory network, such that the bacteria are capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen. The bacteria are not intergeneric microorganisms. Additionally, the bacteria, in planta, produce 1% or more of the fixed nitrogen in the plant.
    Type: Grant
    Filed: January 13, 2021
    Date of Patent: August 29, 2023
    Assignee: Pivot Bio, Inc.
    Inventors: Karsten Temme, Alvin Tamsir, Sarah Bloch, Rosemary Clark, Emily Tung
  • Publication number: 20230257317
    Abstract: Methods and systems are provided for generating and utilizing a genetically engineered bacterium comprising a modification in a gene regulating nitrogen fixation or assimilation, wherein the modification in the gene regulating nitrogen fixation or assimilation results in one or more of: constitutive expression of a nifA gene in nitrogen limiting and non-nitrogen limiting conditions, activity of nifA in non-nitrogen limiting conditions, decreased uridylyl-transferase activity of GlnD, decreased adenylyl-removing activity of GlnE, and increased ammonium excretion.
    Type: Application
    Filed: April 29, 2021
    Publication date: August 17, 2023
    Applicant: Pivot Bio, Inc.
    Inventors: Karsten Temme, Neal Shah, Bilge Ozaydin Eskiyenenturk, Sarah Bloch, Alvin Tamsir
  • Patent number: 11678668
    Abstract: The present disclosure provides non-intergeneric remodeled microbes that are able to fix atmospheric nitrogen and deliver such to plants in a targeted, efficient, and environmentally sustainable manner. The utilization of the taught microbial products will enable farmers to realize more productive and predictable crop yields without the nutrient degradation, leaching, or toxic runoff associated with traditional synthetically derived nitrogen fertilizer. The remodeled microbes taught herein are able to be combined with leading agricultural chemistry and elite germplasm. Furthermore, the disclosure provides seed treatments comprising remodeled microbes.
    Type: Grant
    Filed: January 14, 2022
    Date of Patent: June 20, 2023
    Assignee: PIVOT BIO, INC.
    Inventors: Mark Reisinger, Ernest Sanders, Richard Broglie, Karsten Temme
  • Patent number: 11678667
    Abstract: The present disclosure provides non-intergeneric remodeled microbes that are able to fix atmostpheric nitrogen and deliver such to plants in a targeted, efficient, and environmentally sustainable manner. The utilization of the taught microbial products will enable farmers to realize more productive and predictable crop yields without the nutrient degradation, leaching, or toxic runoff associated with traditional synthetically derived nitrogen fertilizer. The remodeled microbes taught herein are able to be combined with leading agricultural chemistry and elite germplasm. Furthermore, the disclosure provides seed treatments comprising remodeled microbes.
    Type: Grant
    Filed: January 14, 2022
    Date of Patent: June 20, 2023
    Assignee: PIVOT BIO, INC.
    Inventors: Mark Reisinger, Ernest Sanders, Richard Broglie, Karsten Temme
  • Publication number: 20230175959
    Abstract: Systems for plant culture include a chamber featuring one or more walls enclosing a spatial volume internal to the chamber, where the one or more walls include a surface for supporting a plant within the enclosed spatial volume, a gas delivery apparatus with at least one gas source, a nutrient delivery apparatus with a reservoir, a sampling apparatus connected to a port formed in the one or more walls, and a controller configured so that during operation of the system, the controller activates the nutrient delivery apparatus to deliver an aqueous growth medium to the plant, and activates the gas delivery apparatus to deliver into the enclosed spatial volume a mixture of isotopically-substituted gases. Also provided are methods of use of the system for measuring nitrogen in a plant and for identifying microbes capable of providing fixed nitrogen to a plant.
    Type: Application
    Filed: May 1, 2020
    Publication date: June 8, 2023
    Applicant: Pivot Bio, Inc.
    Inventors: Kent Wood, Russell Brown, Jeremy Gage, Rikke Rasmussen, Paul Raine, Sarah Bloch, Alvin Tamsir, Karsten Temme, Bilge Ozaydin Eskiyenenturk
  • Publication number: 20230062568
    Abstract: The present disclosure provides consortia of microbes that are functionally optimized for nitrogen fixation and deliver such to plants in a targeted, efficient, and environmentally sustainable manner. The microbes within the consortium differ in nutrient utilization, temporal occupation, oxygen adaptability, and/or spatial occupation, which enables the microbes to deliver nitrogen to a cereal plant in a spatially targeted (e.g. rhizospheric) and temporally targeted (e.g. during advantageous stages of plant's life cycle) manner. The present disclosure also provides methods of creating a synthetic composition of microbes and methods of using compositions of microbes to fix atmospheric nitrogen and deliver such to a crop.
    Type: Application
    Filed: January 12, 2021
    Publication date: March 2, 2023
    Inventors: Karsten TEMME, Neal SHAH, Bilge OZAYDIN, Sarah BLOCH, Alvin TAMSIR, Daniel FULOP
  • Publication number: 20230033451
    Abstract: The present disclosure provides farmers a new platform for supplying nitrogen to their crops, which is based upon sustainable, biologically fixed nitrogen. The taught platform enables improved yield consistency across all cultivated acreage, irrespective of: weather, environment, or soil conditions. As a result of the increased yield consistency enabled by the taught disclosure, farmers have an increased degree of predictability for yield across each acre they plant, which was not possible with the synthetic nitrogen delivery paradigm of years past.
    Type: Application
    Filed: August 3, 2021
    Publication date: February 2, 2023
    Inventors: Mark REISINGER, Ernest SANDERS, Karsten TEMME
  • Patent number: 11565979
    Abstract: Methods and systems are provided for generating and utilizing a bacterial composition that comprises at least one genetically engineered bacterial strain that fixes atmospheric nitrogen in an agricultural system that has been fertilized with more than 20 lbs of Nitrogen per acre.
    Type: Grant
    Filed: November 15, 2018
    Date of Patent: January 31, 2023
    Assignee: Pivot Bio, Inc.
    Inventors: Karsten Temme, Alvin Tamsir, Sarah Bloch, Rosemary Clark, Emily Tung, Kevin Hammill, Douglas Higgins, Austin Davis-Richardson
  • Publication number: 20220396530
    Abstract: The present disclosure provides non-intergeneric remodeled microbes that are able to fix atmospheric nitrogen and deliver such to plants in a targeted, efficient, and environmentally sustainable manner. The utilization of the taught microbial products will enable farmers to realize more productive and predictable crop yields without the nutrient degradation, leaching, or toxic runoff associated with traditional synthetically derived nitrogen fertilizer, by mitigating or eliminating the need for exogenous nitrogen-containing fertilizers. The remodeled microbes have unique colonization and nitrogen fixation abilities, which enable the microbes to deliver nitrogen to a cereal plant in a spatially targeted (e.g. rhizospheric) and temporally targeted (e.g. during advantageous stages of plants life cycle) manner. The microbes are able to replace the standard agricultural practice of sidedressing and enable a more environmentally sustainable form of farming.
    Type: Application
    Filed: July 11, 2019
    Publication date: December 15, 2022
    Inventors: Alvin TAMSIR, Sarah BLOCH, Mark REISINGER, Ernest SANDERS, Richard BROGLIE, Rosemary CLARK, Karsten TEMME
  • Publication number: 20220211048
    Abstract: A genetically engineered bacterium with a modification in one or more genes selected from: NAC, ptsH, iaaA, gltA, pga, sdiA, fimA1, fimA2, fimA3, fimA4, wzxE, bolA, iscR, fhuF, sodA, sodB, sodC, FNR, arcA, arcB, rpoS, sbnA, treA, treB, phoP, phoQ, yjjPB, ychM, dauA, actP, yusV1, yieL1, yieL2, yieL3, yieL4, pgaB, rafA, melA, uidA, manA, abfA, abnA, lacZ, and yusV2 is disclosed. Methods of use of the genetically engineered bacterium to provide fixed nitrogen to plants, and compositions including the bacterium are also provided.
    Type: Application
    Filed: April 24, 2020
    Publication date: July 7, 2022
    Inventors: Karsten Temme, Alvin Tamsir, Sarah Bloch, Neal Shah, Jenny Johnson, Bilge Ozaydin Eskiyenenturk
  • Publication number: 20220151241
    Abstract: The present disclosure provides non-intergeneric remodeled microbes that are able to fix atmospheric nitrogen and deliver such to plants in a targeted, efficient, and environmentally sustainable manner. The utilization of the taught microbial products will enable farmers to realize more productive and predictable crop yields without the nutrient degradation, leaching, or toxic runoff associated with traditional synthetically derived nitrogen fertilizer. The remodeled microbes taught herein are able to be combined with leading agricultural chemistry and elite germplasm. Furthermore, the disclosure provides seed treatments comprising remodeled microbes.
    Type: Application
    Filed: January 14, 2022
    Publication date: May 19, 2022
    Inventors: Mark REISINGER, Ernest Sanders, Richard Broglie, Karsten Temme
  • Publication number: 20220132861
    Abstract: The present disclosure provides non-intergeneric remodeled microbes that are able to fix atmospheric nitrogen and deliver such to plants in a targeted, efficient, and environmentally sustainable manner. The utilization of the taught microbial products will enable farmers to realize more productive and predictable crop yields without the nutrient degradation, leaching, or toxic runoff associated with traditional synthetically derived nitrogen fertilizer. The remodeled microbes taught herein are able to be combined with leading agricultural chemistry and elite germplasm. Furthermore, the disclosure provides seed treatments comprising remodeled microbes.
    Type: Application
    Filed: January 14, 2022
    Publication date: May 5, 2022
    Inventors: Mark REISINGER, Ernest Sanders, Richard Broglie, Karsten Temme
  • Publication number: 20220127627
    Abstract: The present disclosure provides guided microbial remodeling (GMR) methods for the rational improvement of plant-associated microbes to perform plant-beneficial functions. The GMR methods described herein allow for non-intergeneric genetic optimization of key regulatory networks within the microbes, which improve plant-beneficial functions over wild-type microbes but don't have the risks associated with transgenic approaches (e.g., unpredictable gene function, public and regulatory concerns, etc.). The present disclosure also provides remodeled microbes and compositions thereof. The utilization of remodeled microbes and compositions thereof will enable farmers to realize more productive and predictable crop yields without the nutrient degradation, leaching, or toxic runoff associated with traditional synthetically derived fertilizers.
    Type: Application
    Filed: June 27, 2019
    Publication date: April 28, 2022
    Inventors: Sarah BLOCH, Karsten TEMME, Alvin TAMSIR, Douglas HIGGINS, Austin DAVIS-RICHARDSON, Rosemary CLARK, Shayin GOTTLIEB
  • Publication number: 20220079163
    Abstract: The present disclosure provides non-intergeneric remodeled microbes that are able to fix atmospheric nitrogen and deliver such to plants in a targeted, efficient, and environmentally sustainable manner. The utilization of the taught microbial products will enable farmers to realize more productive and predictable crop yields without the nutrient degradation, leaching, or toxic runoff associated with traditional synthetically derived nitrogen fertilizer. The remodeled microbes taught herein are able to be combined with leading agricultural chemistry and elite germplasm. Furthermore, the disclosure provides seed treatments comprising remodeled microbes.
    Type: Application
    Filed: June 26, 2019
    Publication date: March 17, 2022
    Inventors: Mark REISINGER, Ernest SANDERS, Richard BROGLIE, Karsten TEMME
  • Publication number: 20220017911
    Abstract: Methods of predicting in planta phenotypes of microbial strains are provided. The methods can include culturing microbial strains in a plant exudate medium and assaying in vitro phenotypes of the microbial strains. The methods may also include using the in vitro phenotypes to predict in planta phenotypes of the microbial strains. Methods of using the microbial strains in field trials are also provided.
    Type: Application
    Filed: December 20, 2019
    Publication date: January 20, 2022
    Inventors: Karsten Temme, Alvin Tamsir, Sarah Bloch, Neal Shah, Shayin Gottlieb, Richard Broglie, Emily Tung, Rosemary Clark
  • Publication number: 20210345618
    Abstract: Methods and systems are provided for increasing the phosphate solubilization activity of a microbe. Microbes identified through the use of the disclosed methods and systems are also provided. The methods include the steps of and the systems include instructions for identifying a coding sequence associated with phosphate solubilization, altering the coding sequence associated with phosphate solubilization, functionally linking the altered coding sequence associated with phosphate solubilization to a promoter, and introducing the altered coding sequence associated with phosphate solubilization under the control of the promoter into the microbe. Alterations to the coding sequence can include, e.g., codon randomization of native codons, deletion of coding sequence, and insertion of regulatory sequences.
    Type: Application
    Filed: September 19, 2019
    Publication date: November 11, 2021
    Inventors: Sarah Bloch, Karsten Temme, Alvin Tamsir, Douglas Higgins, Austin Davis-Richardson, Rosemary Clark, Shayin Gottlieb, James Gerard Lorigan
  • Publication number: 20210214282
    Abstract: Disclosed herein are methods of increasing nitrogen fixation in a non-leguminous plant. The methods can comprise exposing the plant to a plurality of bacteria. Each member of the plurality comprises one or more genetic variations introduced into one or more genes or non-coding polynucleotides of the bacteria's nitrogen fixation or assimilation genetic regulatory network, such that the bacteria are capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen. The bacteria are not intergeneric microorganisms. Additionally, the bacteria, in planta, produce 1% or more of the fixed nitrogen in the plant.
    Type: Application
    Filed: January 13, 2021
    Publication date: July 15, 2021
    Inventors: Karsten Temme, Alvin Tamsir, Sarah Bloch, Rosemary Clark, Emily Tung