Abstract: The invention is in the field of analytical technology and relates to an improved procedure for determining the concentration or activity of an analyte in a sample. Specifically the invention provides an automated algorithm for the quality control of (RT-)qPCR reactions. Plotting the fluorescence intensity of a reporter dye divided by the fluorescence intensity of a passive reference dye against the cycle number leads to a so-called sigmoid function which is characterized by a background phase, an exponential growth phase and a plateau phase. Since the fluorescence intensity as a function of cycles relates to the initial number of template molecules in the sample, qPCR curves can be used to quantify the amount of RNA or DNA fragments in the sample by determination of a so-called Cq value.

Abstract: The invention describes how to estimate delta-Cq values from measured (raw-)Cq values gained from PCR measurements and how to calculate confidence intervals for them. This is realized by the following processing steps: A noise model, which might be constructed on some training PCR data, calculates the distribution of the true target material concentration of a single well for an observed measurement results. Said distribution is calculated for all types of measurement results including “Numeric” raw-Cq values as well as Cq being “Undetected”, which denotes that no fluorescence signal was detected during all cycles and thus corresponds to no or very few target molecules.

Abstract: The present invention relates to methods, kits and systems for the prognosis of the disease outcome of breast cancer, said method comprising: (a) determining in a tumor sample from said patient the RNA expression levels of at least 2 of the following 9 genes: UBE2C, BIRC5, RACGAP1, DHCR7, STC2, AZGP1, RBBP8, IL6ST, and MGP (b) mathematically combining expression level values for the genes of the said set which values were determined in the tumor sample to yield a combined score, wherein said combined score is indicative of a prognosis of said patient; and kits and systems for performing said method.

Abstract: The present invention relates to methods for prediction of an outcome of neoplastic disease or cancer. More specifically, the present invention relates to a method for the prediction of breast cancer by determining in a biological sample from said patient an expression level of a plurality of genes selected from the group consisting of ACTG1, CA12, CALM2, CCND1, CHPT1, CLEC2B, CTSB, CXCL13, DCN, DHRS2, EIF4B, ERBB2, ESR1, FBXO28, GABRP, GAPDH, H2AFZ, IGFBP3, IGHG1, IGKC, KCTD3, KIAA0101, KRT17, MLPH, MMP1, NAT1, NEK2, NR2F2, OAZ1, PCNA, PDLIM5, PGR, PPIA, PRC1, RACGAP1, RPL37A, SOX4, TOP2A, UBE2C and VEGF.

Abstract: The present invention relates to an expandable shaft (1) for insertion into a core, the expandable shaft have a longitudinal extension and comprising: a profile (4), which extends in the longitudinal direction of the shaft, the profile comprising a plurality of longitudinal grooves; inflatable and expandable chambers which are adapted to be arranged in the grooves; gripping means (3) which are arranged in connection to the expandable chambers, the gripping means being adapted to radial movement between a first position and a second position in view of an expansion of the inflatable and expandable chambers so that the gripping means (3) are movable in a radial direction between a first position and a second position depending on an expansion of the expandable chambers wherein an outer cylindrical tube is arranged outside the profile (4), the outer cylindrical tube comprising a plurality of openings, and wherein the gripping means (3) are aligned with the openings of the tube so that the gripping means can p

Abstract: The present invention relates to methods for prediction of an outcome of neoplastic disease or cancer. More specifically, the present invention relates to a method for the prediction of breast cancer by determining in a biological sample from said patient an expression level of a plurality of genes selected from the group consisting of ACTG1, CA12, CALM2, CCND1, CHPT1, CLEC2B, CTSB, CXCL13, DCN, DHRS2, EIF4B, ERBB2, ESR1, FBXO28, GABRP, GAPDH, H2AFZ, IGFBP3, IGHG1, IGKC, KCTD3, KIAA0101, KRT17, MLPH, MMP1, NAT1, NEK2, NR2F2, OAZ1, PCNA, PDLIM5, PGR, PPIA, PRC1, RACGAP1, RPL37A, SOX4, TOP2A, UBE2C and VEGF.

Abstract: The invention relates to methods for predicting an outcome of cancer in a patient suffering from cancer, said patient having been previously diagnosed as node positive and treated with cytotoxic chemotherapy, said method comprising determining in a biological sample from said patient an expression level of a plurality of genes selected from the group consisting of ACTG1, CAl2, CALM2, CCND1, CHPT1, CLEC2B, CTSB, CXCL13, DCN, DHRS2, EIF4B, ERBB2, ESR1, FBXO28, GABRP, GAPDH, H2AFZ, IGFBP3, IGHG1, IGKC, KCTD3, KIAA0101, KRT17, MLPH, MMP1, NAT1, NEK2, NR2F2, OAZ1, PCNA, PDLIM5, PGR, PPIA, PRC1, RACGAP1, RPL37A, SOX4, TOP2A, UBE2C and VEGF; ABCB1, ABCG2, ADAM15, AKR1C1, AKR1C3, AKT1, BANF1, BCL2, BIRC5, BRMS1, CASP10, CCNE2, CENPJ, CHPT1, EGFR, CTTN, ERBB3, ERBB4, FBLN1, FIP1L1, FLT1, FLT4, FNTA, GATA3, GSTP1, Herstatin, IGF1R, IGHM, KDR, KIT, CKRT5, SLC39A6, MAPK3, MAPT, MKI67, MMP7, MTA1, FRAP1, MUC1, MYC, NCOA3, NFIB, OLFM1, TP53, PCNA, PI3K, PPERLD1, RAB31, RAD54B, RAF1, SCUBE2, STAU, TINF2, TMSL8, VGLL1, TRA@,

Abstract: An air spring for a vehicle comprises an air bellows that includes an axle-side region and a superstructure-side region movable with respect to one other between a first, adjacent position and a second, spaced-apart position, and a plunger which is arranged on the axle-side region of the air bellows. The air spring further comprising a fastening section adapted to fasten the superstructure-side region of the air bellows to a support element of the vehicle, and a displacement element which is arranged on the fastening section of the air bellows, wherein the displacement element fills at least a substantial portion of the space between the plunger and the fastening section in the first position of the air bellows.

Abstract: A liner emptying device and a liner filling device as well as methods using them for contamination-free filling and emptying of a liner. This contamination-free filling and emptying prevents any danger to the health of the people working with the packaged substances. Both the liner emptying device and the liner filling device are connected to the liner via a fastening device and the residual films sealing off the device can be removed via an access port, whereby the liner can be filled or emptied without contaminating the surroundings.

Abstract: A liner emptying device and a liner filling device as well as methods using them for contamination-free filling and emptying of a liner. This contamination-free filling and emptying prevents any danger to the health of the people working with the packaged substances. Both the liner emptying device and the liner filling device are connected to the liner via a fastening device and the residual films sealing off the device can be removed via an access port, whereby the liner can be filled or emptied without contaminating the surroundings.

Abstract: A device and a method for reducing particle exposure to substrates during thermal treatment are disclosed. Semiconductor wafers may be rotated on a device within a process chamber divided into two partial chambers such that a first partial chamber contains the substrate to be thermally treated and a second partial chamber contains at least parts of the rotation device. Between the partial chambers, a flow of gas is set such that gas from the second partial chamber is substantially prevented from passing into the first partial chamber. In this way, particles which are produced by rotation abrasion in the second partial chamber are largely prevented from passing onto the substrate to be thermally treated. This device and this method are particularly advantageous if the rotation is realized by means of a gas drive, wherein the gas used for the rotation can be introduced directly into the second partial chamber.

Abstract: The aim of the invention is to reduce the formation of scratches in a device for the thermal treatment of substrates, in particular, semiconductor substrates, in a chamber in which the substrate is placed upon support elements. According to the invention, said aim is achieved by means of displaceable support elements.

Abstract: Air bearings support a rotating wafer carrying base in an RTP system. The base in proximity to the air bearing is protected from warping due to absorption of radiation from the hot wafer being treated. The most preferred embodiment splits the base into an inner disk carrying the wafer and an outer ring, where the inner ring which absorbs the most energy contacts and is supported at three points by the outer disk which is supported by the air bearing.

Abstract: An apparatus for preventing warping of a rotatable base for supporting an object being processed in a Rapid Thermal Processing (RTP) system due to non uniform heating of the base by radiation from the object being processed is disclosed.

Abstract: A clamp for arrangement on a carrying arm is described. The clamp has a first and a second clamp half (10, 20) with in each case two lateral angle pieces (12; 21 ), in which are located a hole (14; 23) for positioning the clamp halves (10, 20) on the carrying arm, whereby the angle pieces (12, 21) which are directed against each other lie pairwise against each other and in each case one angle piece (21) of each clamp half (10, 20) lies on the inside and the other angle piece (12) of the same clamp half (10, 20) lies on the outside, and a legged spring (4) which lies between the clamp halves (10, 20) and keeps the clamps (3) closed. The internal angle piece (21) of one clamp half (10; 20) interlocks rotatably with the external angle piece (12) of the other clamp half (20; 10), and the legged spring (4) fills, in axial direction, the space between the internal angle pieces (21) in each case of the clamp halves (10, 20).