Patents by Inventor Kasimalai AZHAKANANDAM
Kasimalai AZHAKANANDAM has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 11643651Abstract: The present invention provides a method of selecting a mRNA for production of a polypeptide of interest comprising: a) producing an array of individual mRNA sequences comprising different nucleotide sequences encoding the polypeptide of interest; b) determining one or more or two or more of the following parameters for each individual mRNA sequence of (a): (i) minimum free energy (MFE) RNA secondary structure; (ii) ensemble free energy (EFE); (iii) frequency of the minimum free energy (FMFE) RNA secondary structure in a thermodynamic ensemble; and (iv) ensemble diversity (ED); c) ranking the individual mRNA sequences of the array according the parameters determined in step (b); and d) selecting a mRNA sequence from the ranked array of step (c), wherein the selected mRNA produces the polypeptide of interest. The present invention further provides a method of selecting a mRNA for enhanced and reduced production of a polypeptide of interest.Type: GrantFiled: February 15, 2017Date of Patent: May 9, 2023Assignee: Syngenta Participations AGInventors: Shib Sankar Basu, Kristen Kamerath Dang, Thomas Z. McNeill, Kasimalai Azhakanandam, Sheng Guo
-
Publication number: 20220325293Abstract: The present invention is directed to promoters that have particular utility in driving root-specific expression of heterologous genes that impart increased agronomic, horticultural and/or pesticidal characteristics to a given transgenic plant. The present invention is also drawn to DNA molecules comprising the promoters of the invention and transformed plant tissues containing DNA molecules comprising a promoter of the invention operably linked to a heterologous gene or genes, and seeds thereof.Type: ApplicationFiled: August 26, 2020Publication date: October 13, 2022Applicant: Syngenta Crop Protection AGInventors: Kasimalai AZHAKANANDAM, Joseph Dallas Clarke, V, Ailing ZHOU, Jared Conville
-
Patent number: 10689664Abstract: The present invention is drawn to a nucleic acid sequence which confers expression of the insecticidal proteins mCry3A and eCry3.1Ab when introduced into a cell.Type: GrantFiled: April 27, 2016Date of Patent: June 23, 2020Assignee: Syngenta Participations AGInventors: Volker Mittendorf, Jared Conville, John Daniel Hipskind, Kasimalai Azhakanandam, Andrew Noe, Xiaoyin Fei, Kevin V. Donohue
-
Publication number: 20180111969Abstract: The present invention is drawn to a nucleic acid sequence which confers expression of the insecticidal proteins mCry3A and eCry3.1Ab when introduced into a cell.Type: ApplicationFiled: April 27, 2016Publication date: April 26, 2018Applicant: SYNGENTA PARTICIPATIONS AGInventors: Volker Mittendorf, Jared Conville, John Daniel Hipskind, Kasimalai Azhakanandam, Andrew Noe, Xiaoyin Fei, Kevin V. Donohue
-
Patent number: 9862960Abstract: Compositions and methods for transiently expressing proteins in a plant are provided. The compositions comprise plants, seeds, plant tissues, and plant parts expressing a protein, wherein the protein is expressed transiently and the transient expression of the protein can be used as a predictive model of how said protein will be expressed in stable transgenic plants in regards to qualitative and quantitative data. The predictive model may be used but is not limited to: promoter evaluation, evaluation of expression cassette construction for best performance (e.g. addition of enhancers or gene silencing suppressors), evaluation of best ways to express heterologous genes (e.g. point mutations, targeting), fast evaluation of endogenous gene knockout, evaluation of protein expression levels, cellular targeting, tissue targeting, transcriptional enhancers, translational enhancer protein toxicity and metabolic profiling. Further provided are methods of use.Type: GrantFiled: December 23, 2013Date of Patent: January 9, 2018Assignee: Syngenta Participations AGInventor: Kasimalai Azhakanandam
-
Publication number: 20170226504Abstract: The present invention provides a method of selecting a mRNA for production of a polypeptide of interest comprising: a) producing an array of individual mRNA sequences comprising different nucleotide sequences encoding the polypeptide of interest; b) determining one or more or two or more of the following parameters for each individual mRNA sequence of (a): (i) minimum free energy (MFE) RNA secondary structure; (ii) ensemble free energy (EFE); (iii) frequency of the minimum free energy (FMFE) RNA secondary structure in a thermodynamic ensemble; and (iv) ensemble diversity (ED); c) ranking the individual mRNA sequences of the array according the parameters determined in step (b); and d) selecting a mRNA sequence from the ranked array of step (c), wherein the selected mRNA produces the polypeptide of interest. The present invention further provides a method of selecting a mRNA for enhanced and reduced production of a polypeptide of interest.Type: ApplicationFiled: February 15, 2017Publication date: August 10, 2017Applicant: Syngenta Participations AGInventors: Shib Sankar BASU, Kristen Kamerath DANG, Thomas Z. MCNEILL, Kasimalai AZHAKANANDAM, Sheng Guo
-
Patent number: 9605258Abstract: The present invention provides a method of selecting a mRNA for production of a polypeptide of interest comprising: a) producing an array of individual mRNA sequences comprising different nucleotide sequences encoding the polypeptide of interest; b) determining one or more or two or more of the following parameters for each individual mRNA sequence of (a): (i) minimum free energy (MFE) RNA secondary structure; (ii) ensemble free energy (EFE); (iii) frequency of the minimum free energy (FMFE) RNA secondary structure in a thermodynamic ensemble; and (iv) ensemble diversity (ED); c) ranking the individual mRNA sequences of the array according the parameters determined in step (b); and d) selecting a mRNA sequence from the ranked array of step (c), wherein the selected mRNA produces the polypeptide of interest. The present invention further provides a method of selecting a mRNA for enhanced and reduced production of a polypeptide of interest.Type: GrantFiled: January 9, 2015Date of Patent: March 28, 2017Assignee: Syngenta Participations AGInventors: Shib Sankar Basu, Kristen Kamerath Dang, Kasimalai Azhakanandam, Thomas Z. McNeill, Sheng Guo
-
Patent number: 9334494Abstract: The present invention provides a method of selecting a mRNA for production of a polypeptide of interest comprising: a) producing an array of individual mRNA sequences comprising different nucleotide sequences encoding the polypeptide of interest; b) determining one or more or two or more of the following parameters for each individual mRNA sequence of (a): (i) minimum free energy (MFE) RNA secondary structure; (ii) ensemble free energy (EFE); (iii) frequency of the minimum free energy (FMFE) RNA secondary structure in a thermodynamic ensemble; and (iv) ensemble diversity (ED); c) ranking the individual mRNA sequences of the array according the parameters determined in step (b); and d) selecting a mRNA sequence from the ranked array of step (c), wherein the selected mRNA produces the polypeptide of interest. The present invention further provides a method of selecting a mRNA for enhanced and reduced production of a polypeptide of interest.Type: GrantFiled: December 7, 2012Date of Patent: May 10, 2016Assignee: SYNGENTA PARTICIPATIONS AGInventors: Shib Sankar Basu, Kristen Kamerath Dang, Kasimalai Azhakanandam, Thomas Z. McNeill, Sheng Guo
-
Patent number: 9068191Abstract: This invention provides methods of transiently expressing a nucleotide sequence in one or more cells of a soybean plant, comprising: a) abrading the surface of a soybean plant and/or intact part thereof; and either (b1) immersing the abraded plant and/or abraded intact part thereof in a solution comprising Agrobacterium cells and a surfactant, wherein the Agrobacterium cells comprise the nucleotide sequence to be transiently expressed; (b2) applying a vacuum to the immersed soybean plant and/or intact part thereof of step (b1); and (b3) releasing the vacuum, thereby introducing the solution into the plant and/or intact part thereof; or (c) contacting the abraded plant and/or abraded intact part thereof with a solution comprising Agrobacterium cells and a surfactant, wherein the Agrobacterium cells comprise the nucleotide sequence to be transiently expressed, whereby the nucleotide sequence is transiently expressed in one or more cells of the plant.Type: GrantFiled: December 15, 2011Date of Patent: June 30, 2015Assignee: Syngenta Participations AGInventors: Kasimalai Azhakanandam, Pei Su
-
Publication number: 20150152408Abstract: The present invention provides a method of selecting a mRNA for production of a polypeptide of interest comprising: a) producing an array of individual mRNA sequences comprising different nucleotide sequences encoding the polypeptide of interest; b) determining one or more or two or more of the following parameters for each individual mRNA sequence of (a): (i) minimum free energy (MFE) RNA secondary structure; (ii) ensemble free energy (EFE); (iii) frequency of the minimum free energy (FMFE) RNA secondary structure in a thermodynamic ensemble; and (iv) ensemble diversity (ED); c) ranking the individual mRNA sequences of the array according the parameters determined in step (b); and d) selecting a mRNA sequence from the ranked array of step (c), wherein the selected mRNA produces the polypeptide of interest. The present invention further provides a method of selecting a mRNA for enhanced and reduced production of a polypeptide of interest.Type: ApplicationFiled: January 9, 2015Publication date: June 4, 2015Inventors: Shib Sankar Basu, Kristen Kamerath Dang, Kasimalai Azhakanandam, Thomas Z. McNeill, Sheng Guo
-
Publication number: 20140123341Abstract: Compositions and methods for transiently expressing proteins in a plant are provided. The compositions comprise plants, seeds, plant tissues, and plant parts expressing a protein, wherein the protein is expressed transiently and the transient expression of the protein can be used as a predictive model of how said protein will be expressed in stable transgenic plants in regards to qualitative and quantitative data. The predictive model may be used but is not limited to: promoter evaluation, evaluation of expression cassette construction for best performance (e.g. addition of enhancers or gene silencing suppressors), evaluation of best ways to express heterologous genes (e.g. point mutations, targeting), fast evaluation of endogenous gene knockout, evaluation of protein expression levels, cellular targeting, tissue targeting, transcriptional enhancers, translational enhancer protein toxicity and metabolic profiling. Further provided are methods of use.Type: ApplicationFiled: December 23, 2013Publication date: May 1, 2014Inventor: Kasimalai Azhakanandam
-
Patent number: 8642839Abstract: Compositions and methods for transiently expressing proteins in a plant are provided. The compositions comprise plants, seeds, plant tissues, and plant parts expressing a protein, wherein the protein is expressed transiently and the transient expression of the protein can be used as a predictive model of how said protein will be expressed in stable transgenic plants in regards to qualitative and quantitative data. The predictive model may be used but is not limited to: promoter evaluation, evaluation of expression cassette construction for best performance (e.g. addition of enhancers or gene silencing suppressors), evaluation of best ways to express heterologous genes (e.g. point mutations, targeting), fast evaluation of endogenous gene knockout, evaluation of protein expression levels, cellular targeting, tissue targeting, transcriptional enhancers, translational enhancer protein toxicity and metabolic profiling. Further provided are methods of use.Type: GrantFiled: June 11, 2010Date of Patent: February 4, 2014Assignee: Syngenta Participations AGInventor: Kasimalai Azhakanandam
-
Publication number: 20130084641Abstract: The present invention provides a method of selecting a mRNA for production of a polypeptide of interest comprising: a) producing an array of individual mRNA sequences comprising different nucleotide sequences encoding the polypeptide of interest; b) determining one or more or two or more of the following parameters for each individual mRNA sequence of (a): (i) minimum free energy (MFE) RNA secondary structure; (ii) ensemble free energy (EFE); (iii) frequency of the minimum free energy (FMFE) RNA secondary structure in a thermodynamic ensemble; and (iv) ensemble diversity (ED); c) ranking the individual mRNA sequences of the array according the parameters determined in step (b); and d) selecting a mRNA sequence from the ranked array of step (c), wherein the selected mRNA produces the polypeptide of interest. The present invention further provides a method of selecting a mRNA for enhanced and reduced production of a polypeptide of interest.Type: ApplicationFiled: December 7, 2012Publication date: April 4, 2013Applicant: Syngenta Participations AGInventors: Shib Sankar Basu, Kristern Kamerath Dang, Kasimalai Azhakanandam, Thomas Z. McNeill, Sheng Guo
-
Publication number: 20120185969Abstract: Compositions and methods for increasing expression of a polypeptide of interest in a plant or plant part thereof are provided. Compositions of the invention are polynucleotide constructs comprising (a) at least one translational enhancer element derived from a virus tandemly stacked with at least one translational enhancer element derived from a cellular gene, and (b) an operably linked polynucleotide encoding a polypeptide of interest. Expression cassettes, vectors, and transgenic plants and plant parts comprising these polynucleotide constructs are also provided. Methods for increasing expression of a polypeptide of interest in a plant or plant part thereof utilizing the polynucleotide constructs and expression cassettes of the invention are also provided.Type: ApplicationFiled: September 2, 2010Publication date: July 19, 2012Inventors: Andrew DeBrecht, Kasimalai Azhakanandam
-
Publication number: 20120174262Abstract: This invention provides methods of transiently expressing a nucleotide sequence in one or more cells of a soybean plant, comprising: a) abrading the surface of a soybean plant and/or intact part thereof; and either (b1) immersing the abraded plant and/or abraded intact part thereof in a solution comprising Agrobacterium cells and a surfactant, wherein the Agrobacterium cells comprise the nucleotide sequence to be transiently expressed; (b2) applying a vacuum to the immersed plant and/or intact part thereof of step (b1); and (b3) releasing the vacuum, thereby introducing the solution into the plant and/or intact part thereof; or (c) contacting the abraded plant and/or abraded intact part thereof with a solution comprising Agrobacterium cells and a surfactant, wherein the Agrobacterium cells comprise the nucleotide sequence to be transiently expressed, whereby the nucleotide sequence is transiently expressed in one or more cells of the plant.Type: ApplicationFiled: December 15, 2011Publication date: July 5, 2012Inventors: Kasimalai Azhakanandam, Pei Su
-
Publication number: 20120096583Abstract: The present invention provides a method for selecting an intron for enhanced expression of a nucleotide sequence encoding a polypeptide, comprising: a) determining a mean free energy value per base pair of one or more introns; b) determining a mean IMEter score of the one or more introns of (a); and c) selecting an intron having a mean free energy value per base pair of below about ?0.268 kcal/mol/bp and a mean IMEter score of at least about ?0.034/bp, thereby selecting an intron for enhanced expression of a nucleotide sequence encoding the polypeptide, wherein expression of a nucleotide sequence operably associated with the selected intron is enhanced as compared to expression of a nucleotide sequence not operably associated with an intron having a mean free energy value per base pair of below about ?0.268 kcal/mol/bp and a mean IMEter score of at least about ?0.034/bp.Type: ApplicationFiled: September 15, 2011Publication date: April 19, 2012Inventors: Kasimalai Azhakanandam, Thomas Z. McNeill
-
Publication number: 20100319089Abstract: Compositions and methods for transiently expressing proteins in a plant are provided. The compositions comprise plants, seeds, plant tissues, and plant parts expressing a protein, wherein the protein is expressed transiently and the transient expression of the protein can be used as a predictive model of how said protein will be expressed in stable transgenic plants in regards to qualitative and quantitative data. The predictive model may be used but is not limited to: promoter evaluation, evaluation of expression cassette construction for best performance (e.g. addition of enhancers or gene silencing suppressors), evaluation of best ways to express heterologous genes (e.g. point mutations, targeting), fast evaluation of endogenous gene knockout, evaluation of protein expression levels, cellular targeting, tissue targeting, transcriptional enhancers, translational enhancer protein toxicity and metabolic profiling. Further provided are methods of use.Type: ApplicationFiled: June 11, 2010Publication date: December 16, 2010Inventor: Kasimalai AZHAKANANDAM