Abstract: A method of imaging a sample providing light from a light source, directing the provided light into an extended focus, scanning the extended focus across a wavefront modulating element that modulates amplitudes of the light along the extended focus, providing the modulated light to the sample, detecting light emitted from the sample in response to excitation by the modulated light, and generating an image of the sample based on the detected fluorescence emission light.

Abstract: Techniques are described for imaging a sample where the techniques include acquiring a raster scan image of the sample, providing light from a light source, directing the light into a plurality of different light beam paths at different times, providing light in each of the plurality of light beam paths through an objective lens to the sample, and providing light in each of the plurality of beams to different locations within the sample. Fluorescence emission light from the sample is detected in response to excitation by light in each of the plurality of light beam paths, where the detected fluorescence emission light corresponds to fluorescence intensity projections of the sample with low mutual coherence, and an image of the sample is generated based on the detected fluorescence emission light and based on the raster scan image.

Abstract: Techniques are described for imaging a sample where the techniques include acquiring a raster scan image of the sample, providing light from a light source, directing the light into a plurality of different light beam paths at different times, providing light in each of the plurality of light beam paths through an objective lens to the sample, and providing light in each of the plurality of beams to different locations within the sample. Fluorescence emission light from the sample is detected in response to excitation by light in each of the plurality of light beam paths, where the detected fluorescence emission light corresponds to fluorescence intensity projections of the sample with low mutual coherence, and an image of the sample is generated based on the detected fluorescence emission light and based on the raster scan image.