Abstract: Aspects of the present disclosure relates to genetically altered LysM receptors. In particular, the present disclosure relates to a hydrophobic patch into the LysM2 domain which can increase affinity and/or selectivity for LCOs and by replacement of regions in the LysM1 domain with the corresponding regions of the LysM1 domain from a donor LysM receptor that can alter the affinity and/or selectivity for the oligosaccharide particularly for LCOs and can alter the specificity between LCO when using regions from a high affinity and specificity LCO LysM receptor such as a legume NFR1 receptor. The present disclosure also relates to genetically altering LysM receptors in plants to include a hydrophobic patch or alter the hydrophobic patch and to genetically altering LysM receptors in plants by replacement of regions in the LysM2 domain.

Abstract: Aspects of the present disclosure relates to genetically altered LysM receptors. In particular, the present disclosure relates to a hydrophobic patch into the LysM2 domain which can increase affinity and/or selectivity for LCOs and by replacement of regions in the LysM1 domain with the corresponding regions of the LysM1 domain from a donor LysM receptor that can alter the affinity and/or selectivity for the oligosaccharide particularly for LCOs and can alter the specificity between LCO when using regions from a high affinity and specificity LCO LysM receptor such as a legume NFR1 receptor. The present disclosure also relates to genetically altering LysM receptors in plants to include a hydrophobic patch or alter the hydrophobic patch and to genetically altering LysM receptors in plants by replacement of regions in the LysM2 domain.

Abstract: The present disclosure relates to modified plant SYMRK polypeptides that constitutively induces symbiotic organogenesis or induces symbiotic organogenesis in the absence of rhizobial bacteria and/or arbuscular mycorrhizal fungi recognized by the plant at a higher level than the unmodified plant SYMRK polypeptide under the same conditions and use thereof in plants.

Abstract: The present disclosure relates to genetically modified plant LysM receptors and methods of producing the same. In particular, the present disclosure relates to modified plant LysM receptors including a modified juxtamembrane (JM) zone 4, and optionally further including a modified JM zone 2, a modified JM zone 3, a modified extracellular domain, and/or a modified kinase C-terminus region or a modified kinase N-terminus region. The modified LysM receptors of the present disclosure are either able to able to initiate NFR1-mediated root nodule symbiosis signaling or able to initiate ROS signaling. In addition, the present disclosure relates to genetically modified plants or parts thereof including the genetically modified plant LysM receptors and methods of producing the same. The present disclosure further relates to expression vectors, isolated DNA molecules, or recombinant nucleic acids encoding the genetically modified plant LysM receptors.

Abstract: The present disclosure relates to modified plant LysM receptor polypeptides with modified intracellular domains, specifically, modified ?A motifs and/or modified ?A? motifs in the juxtamembrane domain. The present disclosure further relates to genetically modified plants including the modified plant LysM receptor polypeptides, and methods of producing the same.

Abstract: The present disclosure relates to enhancing nitrogen fixation in legumes grown under high nitrate or nitrate stress conditions. In particular, the present disclosure relates to genetically modified plants with altered level or expression of FUN or FUN downstream targets, and methods of producing and growing the same. The present disclosure further relates to nodule senescence controlled by FUN and its downstream targets, as well as the regulation of FUN activity or expression by cellular zinc.

Abstract: The present disclosure relates to synthetic approaches based on affinity peptides to drive assembly and activation of transmembrane receptor complexes. The present disclosure further relates to the core NFR1-NFR5 receptor complex initiating the cortical root nodule organogenesis program and the epidermal program, as well as the identification of barley receptor complexes that function in root nodule symbiosis.

Abstract: Aspects of the present disclosure relate to genetically altered plants having a heterologous EPR3 or EPR3-like polypeptide or a modified EPR3 or EPR3-like polypeptide and/or having a heterologous EPR3a or EPR3a-like polypeptide or a modified EPR3a or EPR3a-like polypeptide, wherein the EPR3 or EPR3-like polypeptide and/or the EPR3a or EPR3a-like polypeptide provide increased selectivity for a beneficial commensal microbe as compared to a wild-type plant under the same conditions. Other aspects of the present disclosure relate to methods of making such plants as well as cultivating these genetically altered plants. Additional aspects of the present disclosure relate to methods of identifying a beneficial commensal microbe capable of interacting with a plant root microbiota.

Abstract: Aspects of the present disclosure relate to genetically altered LysM receptors. In particular, the present disclosure relates replacement of part or all of motifs in the LysM1 domain with the corresponding motifs of the LysM1 domain from a donor LysM receptor that can alter the affinity, selectivity, and/or specificity for an oligosaccharide, particularly for Nod factors (lipochitooligosaccharides (LCOs)). The present disclosure also relates to genetically altering LysM receptors in plants to include a modified LysM1 domain and to genetically altering LysM receptors in plants by replacement of part or all of motifs in the LysM1 domain. The present disclosure further relates to combining LysM1 domain modifications with modifications of LysM2 domains to include a hydrophobic patch or alter the hydrophobic patch, whereby the LysM2 domain modifications can alter the affinity, selectivity, and/or specificity for an oligosaccharide, particularly for Nod factors (lipochitooligosaccharides (LCOs)).

Abstract: Aspects of the present disclosure relates to genetically altered LysM receptors. In particular, the present disclosure relates to a hydrophobic patch into the LysM2 domain which can increase affinity and/or selectivity for LCOs and by replacement of regions in the LysM1 domain with the corresponding regions of the LysM1 domain from a donor LysM receptor that can alter the affinity and/or selectivity for the oligosaccharide particularly for LCOs and can alter the specificity between LCO when using regions from a high affinity and specificity LCO LysM receptor such as a legume NFR1 receptor. The present disclosure also relates to genetically altering LysM receptors in plants to include a hydrophobic patch or alter the hydrophobic patch and to genetically altering LysM receptors in plants by replacement of regions in the LysM2 domain.

Abstract: Aspects of the present disclosure relate to genetically modified plants comprising a nucleic acid sequence encoding a heterologous receptor polypeptide. The plants are able to recognize lipo-chitooligosaccharides (LCOs) through the heterologous receptor polypeptide. Other aspects of the present disclosure relate to methods of making such plants.