Abstract: The present invention is in the field of plant molecular biology and is directed to excision of recombinant DNA from the genome of plant somatic cells.

Abstract: The present invention relates to the field of increasing genetic diversity in a targeted way. In particular, it relates to the provision of methods and means for targeted sequence diversification using base editors with an expanded mutation spectrum, including the provision of Cas12a diversifying base editing systems, and uses thereof.

Abstract: The present invention relates to an adenine base editor (ABE), and components thereof. The present invention also relates to a complex comprising an adenine base editor (ABE) and a guide RNA in a functionally associated form. The present invention further relates to a nucleic acid molecule encoding the ABE/guide RNA, an expression construct or a vector comprising a nucleic acid sequence encoding the adenine base editor and/or the nucleic acid sequence encoding the guide RNA. The present invention further relates to a cell comprising an adenine base editor (ABE) and a method of adenine base editing of a target site in a genome of interest in at least one cell of a prokaryotic organism, including bacterial and archaeal organisms, or eukaryotic organism. Besides that, the present invention relates to various methods, kits and uses associated with the ABEs provided.

Abstract: The present disclosure relates to the field of gene genome editing. In particular, it relates to the provision of a CRISPR-Cas class 2 type V prime editing system, including a prime editor, a prime editor complex, a prime editing guide RNA system as well as the means and methods for the modification of a nucleic acid sequence of interest with the prime editing system of the present invention.

Abstract: The present invention relates to the field of plant molecular biology and provides materials and methods for modulating expression of a gene of interest in plants. In particular, the invention provides modified plant promoters or modified coding sequences having increased expression, for example, in developing spikes as well as methods for producing promoters or coding sequences having increased expression. The modified promoters comprise i) at least one binding site for an EIL3 transcription factor and/or at least one binding site for a PHD transcription factor and/or ii) one or more enhancer elements. Moreover, the present invention concerns a nucleic acid molecule encoding a functional restorer polypeptide for wheat G-type cytoplasmic male sterility comprising in the coding sequence a mutated microRNA (“miRNA”) binding site. In some embodiments, said nucleic acid molecule is operably linked to the modified promoter of the present invention.

Abstract: The present invention is in the field of plant molecular biology and is directed to a method for improved screening for known edits within the genome of a cell. The method of the invention comprises dividing a population of plant cells comprising a desired nucleic acid sequence, into subgroups, quantifying the concentration of the desired nucleic acid sequence for each sub-group, culturing cells from the a sub-group(s), dividing the cells into subgroups, quantifying the concentration of the desired nucleic acid sequence in each subgroup, and regenerating intact individual plants from the cells of a selected subgroup(s) with the desired nucleic acid sequence.

Abstract: The present invention relates to the field of gene genome editing. In particular, it relates to the provision of a Cas12a enzyme having nickase activity, as well as the means and methods for the modification of a genomic locus of interest with a Cas12a enzyme having nickase activity and uses thereof.

Abstract: The present invention is in the field of genome editing and is directed to a method for the seam-less introduction of targeted precise modifications in genomic DNA of wheat.

Abstract: Genome editing including the introducing of precise gene edits is well established in diploid plants. Methods well established in the art introduce double strand DNA breaks in the genome of a plant applying technologies such as Zn-finger nucleases, homing endonucleases, TALEN or RNA guided nuclease e.g. Cas9 or Cas12a.

Abstract: Improved methods and means are provided to modify in a targeted manner the genome of a plant cell or plant at a predefined site via bacterial transformation.

Abstract: Methods and means are provided to modify in a targeted manner the genome of a cotton plant using a double stranded DNA break inducing enzyme and embryogenic callus.

Abstract: The present invention relates to the field of agriculture. In particular, the invention provides a thermostable Rca proteins, a recombinant gene, plants comprising the recombinant genes and a method to improve thermotolerance of a cereal plant under stress conditions.

Abstract: Improved methods and means are provided to modify in a targeted manner the genome of a plant cell at a predefined site using a nucleotide-guided DNA modifying polypeptide such as a RNA-guided endonuclease, a guide-polynucleotide and a donor molecule for repair of the DNA break.

Abstract: Improved methods and means are provided to modify in a targeted manner the genome of a eukaryotic cell at a predefined site using a double stranded break inducing enzyme such as a TALEN and a donor molecule for repair of the double stranded break.

Abstract: Improved methods and means are provided to modify in a targeted manner the genome of a plant cell or plant at a predefined site via bacterial transformation.

Abstract: Methods and means are provided to modify in a targeted manner the genome of a cotton plant using a double stranded DNA break inducing enzyme and embryogenic callus.