Abstract: The invention provides a system and methods of multiplexed, solid-phase isothermal nucleic acid amplification. In various aspects, the invention uses a microfluidic device that includes a field of actuatable microposts in a reaction (or assay) chamber to enhance fluid flow, mixing, and hybridization/capture efficiency in a solid-phase capture assay. In various other aspects, the invention uses oligonucleotide primers immobilized in a field of actuatable microposts in a reaction chamber of a microfluidics device for capture and amplification of target-specific nucleic acids in a sample fluid. The invention provides methods of producing a micropost field (array) on a substrate for printing of a capture array (e.g., an array of primer spots). The invention also provides methods of printing an array of capture spots (e.g., primer spots) on the substrate surface of a micropost field.

Abstract: Methods of surface modification of silicones for specific target and high efficiency binding are disclosed. Namely, surface-attached microposts and methods of functionalizing the surface-attached microposts for target-specific analyte capture are provided. For example, a microposts processing platform is provided that is based on a microfluidic flow cell structure that includes a reaction (or assay) chamber. The method utilizes the microposts processing platform that includes an arrangement of surface-attached microposts on at least one surface of the reaction (or assay) chamber. Methods of functionalizing the surface-attached microposts include one or more steps, wherein the incorporation of one or more functionalizing agents are used to provide a micropost surface for target-specific analyte capture.