Abstract: Provided herein is a reaction mixture comprising Cas9 and a non-ionic surfactant, e.g., a polyoxyethylene surfactant. In certain embodiments, the reaction mixture may comprise a Cas9 protein, a guide RNA, a salt, a buffering agent, a nucleic acid target and a non-ionic surfactant. Kits are also provided. In certain embodiments, a kit may comprise: a Cas9 protein; and a concentrated reaction buffer comprising salt, a buffering agent and a non-ionic surfactant.

Abstract: Provided herein is a reaction mixture comprising Cas9 and a non-ionic surfactant, e.g., a polyoxyethylene surfactant. In certain embodiments, the reaction mixture may comprise a Cas9 protein, a guide RNA, a salt, a buffering agent, a nucleic acid target and a non-ionic surfactant. Kits are also provided. In certain embodiments, a kit may comprise: a Cas9 protein; and a concentrated reaction buffer comprising salt, a buffering agent and a non-ionic surfactant.

Abstract: The present invention provides a detection reagent for detection of the presence of a substance of interest in a sample. The detection reagent comprises a binding portion, a linking portion, and a catalytic portion. The linking portion comprises a cleavage site for cleavage of the binding portion from the catalytic portion. According to the method, the detection reagent is caused to bind to the substance of interest. The bound reagent is then cleaved by breaking of a bond in the linking portion. Upon cleavage, the catalytic portion is removed from the binding reaction mixture and caused to catalyze a reaction that produces a detectable product.

Abstract: The invention relates to recombinant polynucleotides encoding the Green Fluorescent Protein (GFP) from R. reniformis, as well as polynucleotides encoding variants and fusion polypeptides of R. reniformis GFP. The invention further relates to vectors encoding R. Reniformis GFP and variants and fusions thereof, as well as to cells comprising and/or expressing such vectors. The invention also relates to recombinant R. reniformis GFP polypeptides and fusion polypeptides and variants thereof, as well as to methods of making and using such polypeptides both in vivo and in vitro.

Abstract: The invention relates to recombinant polynucleotides encoding the Green Fluorescent Protein (GFP) from R. reniformis, as well as polynucleotides encoding variants and fusion polypeptides of R. reniformis GFP. The invention further relates to vectors encoding R. Reniformis GFP and variants and fusions thereof, as well as to cells comprising and/or expressing such vectors. The invention also relates to recombinant R. reniformis GFP polypeptides and fusion polypeptides and variants thereof, as well as to methods of making and using such polypeptides both in vivo and in vitro.