Patents by Inventor Katherine R. Rowlyk

Katherine R. Rowlyk has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Mineral hot start PCR method
    Publication number: 20030082567
    Abstract: The present invention provides a method of performing hot start PCR reactions. The method is based on sequestration of magnesium ions in the form of a precipitate which renders a DNA polymerase inactive until the appropriate time in the PCR reaction when a certain temperature is reached and the magnesium ions are released from the precipitate. Also provided are kits comprising reagents and instructions for amplifying a target nucleic acid by the magnesium precipitate hot start.
    Type: Application
    Filed: March 6, 2002
    Publication date: May 1, 2003
    Inventors: Wayne M. Barnes, Katherine R. Rowlyk
  • Magnesium precipitate methods for magnesium dependent enzymes
    Publication number: 20030027196
    Abstract: The present invention provides methods of performing enzymatic reactions which require the use of magnesium dependent enzymes, including restriction endonucleases, ligases, and reverse transcriptases. The method is based on sequestration of magnesium ions in the form of a precipitate which renders a magnesium dependent enzyme inactive until the appropriate time in the reaction when a certain temperature is reached and the magnesium ions are released from the precipitate. Also provided are kits comprising reagents and instructions for DNA digestion and ligation, and for reverse transcription of RNA into cDNA. Furthermore, the kits and reagents of the present invention can be utilized in other reactions requiring magnesium dependent enzymes.
    Type: Application
    Filed: August 2, 2002
    Publication date: February 6, 2003
    Inventors: Wayne M. Barnes, Katherine R. Rowlyk
  • Magnesium precipitate hot start method for PCR
    Patent number: 6403341
    Abstract: The present invention provides a method of performing hot start PCR reactions. The method is based on sequestration of magnesium ions in the form of a precipitate which renders a DNA polymerase inactive until the appropriate time in the PCR reaction when a certain temperature is reached and the magnesium ions are released from the precipitate. Also provided are kits comprising reagents and instructions for amplifying a target nucleic acid by the magnesium precipitate hot start.
    Type: Grant
    Filed: August 2, 2001
    Date of Patent: June 11, 2002
    Inventors: Wayne M. Barnes, Katherine R. Rowlyk