Abstract: We describe a Cricetulus griseus cell which is modified, preferably genetically engineered, so that the expression of a heat shock protein is up-regulated compared to a cell which has not been so modified, as well as a method of expressing a recombinant protein from a host comprising such an engineered cell. Novel CHO heat shock protein sequences SEQ ID NO: 2 and SEQ ID NO: 4, as well as nucleic acid sequences SEQ ID NO: 1 and SEQ ID NO: 3 are also disclosed.

Abstract: The present invention provides a method for manufacturing a photochromic article having a photochromic compound containing layer. The method includes the step of coating a casting face of at least one mould section with a photochromic host layer. The photochromic host layer is treated to minimise damage during subsequent steps, and a mould is then assembled so that it includes the mould section having the photochromic host layer. The mould is then filled with a photochromic article monomer composition and the monomer composition is subsequently cured to form a photochromic article substrate adhered to the photochromic host layer. The photochromic compound is introduced into the photochromic host layer.