Abstract: A method is provided for automatically discerning between object and non-object pixels in a hyperspectral image data cube. In particular embodiments, the object of the method is a plant, plant part, plant trait, plant phenotype, plant pot or a plant medium. The method comprises a first step of providing a partial least squares discriminant analysis (PLSDA) algorithm and a second step of applying the PLSDA algorithm to a hyperspectral image data cube to automatically determine which pixels contain the spectral properties of the object. The PLSDA algorithm of the method can be generated by establishing a training matrix, performing an eigenvector decomposition of the training matrix, experimentally determining a weighted linear combination of object signal-containing eigenvectors, calculating a regression vector using the weighted linear combination of signal-containing eigenvectors, generating a mask matrix and multiplying the mask matrix by the hyperspectral image data cube along two spatial dimensions.

Abstract: The present invention provides compositions and methods for regulating expression of isolated nucleotide sequences in a plant. The compositions are novel nucleic acid sequences for seed-preferred regulatory sequences. Methods for expressing an isolated nucleotide sequence in a plant using the regulatory sequences are also provided. The methods comprise transforming a plant cell to contain an isolated nucleotide sequence operably linked to the seed-preferred regulatory sequences of the present invention and regenerating a stably transformed plant from the transformed plant cell.

Abstract: The invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are novel nucleotide sequences for seed-preferred promoters isolated from genes for end1 and end2. A method for expressing a heterologous nucleotide sequence in a plant using the disclosed promoter sequences is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one of the promoters of the invention and regenerating a stably transformed plant from the transformed plant cell.

Abstract: The invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are novel nucleotide sequences for seed-preferred promoters isolated from genes for end1 and end2. A method for expressing a heterologous nucleotide sequence in a plant using the disclosed promoter sequences is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one of the promoters of the invention and regenerating a stably transformed plant from the transformed plant cell.

Abstract: The invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are nucleotide sequences for seed-preferred promoters isolated from genes for end1 and end2. A method for expressing a heterologous nucleotide sequence in a plant using the disclosed promoter sequences is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one of the promoters of the invention and regenerating a stably transformed plant from the transformed plant cell.

Abstract: The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are novel nucleotide sequences for seed-preferred promoters isolated from genes for Cim1 (cytokinin-induced message). cZ19B1 (maize 19 kDa zein); or mi1ps (myo-inositol-1-phosphate synthase). A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one of the seed-preferred promoters of the present invention and regenerating a stably transformed plant from the transformed plant cell.