Abstract: A method of modifying a radiation characteristic of an excited emitter (2) and a layer structure (1) therefore, wherein the emitter (2) is placed in the vicinity of a layer structure (1) comprising a metal material, such that the emitter (2) couples to a surface state of the layer structure (1), in particular a surface plasmon polariton, which modifies the radiation characteristic of the emitter (2), wherein the layer structure (1) comprises a metal layer (3) sandwiched between a non-metal superstrate layer (4) and a non-metal substrate layer (5), wherein at least the metal layer (3) and the superstrate layer (4) are separated by a smooth interface (8) with a root mean square roughness equal to or less than 1 nanometer, and wherein the metal layer (3) has a thickness of between 1/100 and 1/20 in relation to an emission wavelength (??) of the emitter (2).

Abstract: A method of fluorescence measurement on analytes, marked with different fluorescent markers, which have spectrally different fluorescence emissions, in a sample is described, having the following steps: illumination of the sample in the measurement volume using a laser to excite the fluorescence emission of the at least two fluorescent markers, the illumination of the measurement volume being performed with a maximum of one single laser line, having an excitation intensity sufficiently high that the fluorescent markers are jointly excited through 2-photon absorptions, detection of the fluorescence emission using at least two detector devices which are designed for light detection in different spectral ranges in accordance with the spectral fluorescence properties of the fluorescent markers, and performance of a cross-correlation and/or coincidence analysis of detector signals of the detector devices. A measurement device for performing the method is also described.

Abstract: A method of fluorescence measurement on analytes, marked with different fluorescent markers, which have spectrally different fluorescence emissions, in a sample is described, having the following steps: illumination of the sample in the measurement volume using a laser to excite the fluorescence emission of the at least two fluorescent markers, the illumination of the measurement volume being performed with a maximum of one single laser line, having an excitation intensity sufficiently high that the fluorescent markers are jointly excited through 2-photon absorptions, detection of the fluorescence emission using at least two detector devices which are designed for light detection in different spectral ranges in accordance with the spectral fluorescence properties of the fluorescent markers, and performance of a cross-correlation and/or coincidence analysis of detector signals of the detector devices. A measurement device for performing the method is also described.