Abstract: The present invention relates to methods for the detection of a sexually transmitted infectious pathogens in human subjects. The detection can be done without sample purification and on several types of pathogens. The methods are ideal for urine samples. One pathogen is Chlamydia trachomatis.

Abstract: A microfluidic test device has a body, a first chamber having an outlet provided with a first valve and holding a first buffer having a first buffer volume, a primary reaction chamber, a sample inlet for receiving and feeding a sample having a sample volume, into the microfluidic test device, a first fluid path connecting the outlet of the first chamber and the sample inlet, a second fluid path connecting the sample inlet and the primary reaction chamber, a primary test part having a primary test chamber, a third primary fluid path connecting the primary reaction chamber and the primary test part, a primary valve arranged in the third primary fluid path, a flow driving device configured to move fluid from the primary reaction chamber to the primary test part, and a heating assembly configured to heat a reaction fluid in the primary reaction chamber.

Abstract: Current invention is directed for rapid sample pretreatment method that allows highly sensitive and specific detection of target nucleic acid (eg human genomic DNA, human pathogen genomic DNA, human non-pathogen genomic DNA) by amplification directly from crude unpurified biological samples lysates (eg human urine, saliva, blood, urethra and cervical swabs and other samples containing biological material). Invention is focused on the description of the biological sample pretreatment method that enables fast release of the genomic material from human and pathogen cells, components of what are compatible with the following nucleic acid amplification method.