Patents by Inventor Katrin Sparbier
Katrin Sparbier has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230159977Abstract: The invention relates to methods for spectrometric characterization of a test cell substrate. The characterization comprises taxonomic classification and determination of a property of interest of the test cell substrate. The characterization may be based on mass-spectrometric measurement data. The property of interest may be a resistance or susceptibility to a growth-influencing factor. After comparing first spectrometric measurement data of the test cell substrate with a provided reference library, a sub-library is created comprising those reference datasets from the reference library that are classified as allowing a taxonomic classification of the test cell substrate. Second spectrometric measurement data after a second preparation of the test cell substrate under conditions that serve to determine a property of interest of the test cell substrate is compared with the sub-library and allow a reliable determination of the property of interest.Type: ApplicationFiled: November 16, 2022Publication date: May 25, 2023Inventor: Katrin SPARBIER
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Publication number: 20220276140Abstract: The invention relates to the preparation of biological cells for the mass spectrometric analysis of cellular properties such as taxonomic classification, antibiotic resistances, response to drugs or other active substances, and others. The cells can be prokaryotic or eukaryotic microorganisms which have particularly been cultivated directly on a mass spectrometric sample support, or eukaryotic cells from tissues or cell cultures. The invention proposes that the cells are not disrupted by adding matrix solution for a subsequent ionization by matrix-assisted laser desorption (MALDI), but that they are disrupted in a separate treatment step using acids and/or solvents on the sample support itself. Surprisingly, the cell proteins released then adhere to the sample support so that they can be carefully washed with buffer solution to remove salts and other soluble impurities which can stem from earlier treatment steps, for example from nutrient solution.Type: ApplicationFiled: March 23, 2022Publication date: September 1, 2022Inventors: Katrin SPARBIER, Beatrix WEGEMANN
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Patent number: 11307122Abstract: The invention relates to the preparation of biological cells for the mass spectrometric analysis of cellular properties such as taxonomic classification, antibiotic resistances, response to drugs or other active substances, and others. The cells can be prokaryotic or eukaryotic microorganisms which have particularly been cultivated directly on a mass spectrometric sample support, or eukaryotic cells from tissues or cell cultures. The invention proposes that the cells are not disrupted by adding matrix solution for a subsequent ionization by matrix-assisted laser desorption (MALDI), but that they are disrupted in a separate treatment step using acids and/or solvents on the sample support itself. Surprisingly, the cell proteins released then adhere to the sample support so that they can be carefully washed with buffer solution to remove salts and other soluble impurities which can stem from earlier treatment steps, for example from nutrient solution.Type: GrantFiled: May 1, 2018Date of Patent: April 19, 2022Inventors: Katrin Sparbier, Beatrix Wegemann
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Patent number: 11299763Abstract: The invention relates to methods and instruments for determining the resistances of microbes to antibiotics, in particular those microbes which produce beta-lactamases. The method determines the resistance of the microbes in less than an hour by incubating a tiny quantity of the microbes on a mass spectrometric sample support plate after they have been combined with a dosed quantity of a suitable antibiotic, for example the beta-lactam antibiotic imipenem, and by direct mass spectrometric measurement of the breakdown of the antibiotic by the microbial enzymes.Type: GrantFiled: November 4, 2019Date of Patent: April 12, 2022Inventors: Katrin Sparbier, Beatrix Wegemann
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Patent number: 11142784Abstract: The invention relates to a mass spectrometric method to determine microbial resistances to antibiotics. The decrease or modification of specific nutrient components by microbes, and thus the metabolism of the microbes, is determined mass spectrometrically in culture media containing antibiotics. Hence it is not the microbes which are introduced into the mass spectrometric analysis, but the culture medium. The special nutrient components which are subject to the mass spectrometric observation are indicators for the metabolism exhibited by the microbes in the culture in the presence of antibiotics, and are thus indicators for their susceptibility or resistance.Type: GrantFiled: January 14, 2015Date of Patent: October 12, 2021Inventors: Markus Kostrzewa, Katrin Sparbier
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Patent number: 10913968Abstract: The invention relates to a mass-spectrometric method to determine microbial resistances to antibiotics, in which the microbes are cultured in a medium comprising an antibiotic, and a mass spectrum of the microbes is acquired after they have been cultured. The method is characterized by the fact that any microbial growth taking place during the culture is mass-spectrometrically determined with the aid of a reference substance, which is added in a dosed amount and is co-measured in the mass spectrum, wherein a growth in microbes indicates the resistance to the antibiotic.Type: GrantFiled: June 15, 2018Date of Patent: February 9, 2021Inventors: Christoph Lange, Katrin Sparbier
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Publication number: 20200080127Abstract: The invention relates to methods and instruments for determining the resistances of microbes to antibiotics, in particular those microbes which produce beta-lactamases. The method determines the resistance of the microbes in less than an hour by incubating a tiny quantity of the microbes on a mass spectrometric sample support plate after they have been combined with a dosed quantity of a suitable antibiotic, for example the beta-lactam antibiotic imipenem, and by direct mass spectrometric measurement of the breakdown of the antibiotic by the microbial enzymes.Type: ApplicationFiled: November 4, 2019Publication date: March 12, 2020Inventors: Katrin SPARBIER, Beatrix WEGEMANN
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Patent number: 10480020Abstract: The invention relates to methods and instruments for determining the resistances of microbes to antibiotics, in particular those microbes which produce beta-lactamases. The method determines the resistance of the microbes in less than an hour by incubating a tiny quantity of the microbes on a mass spectrometric sample support plate after they have been combined with a dosed quantity of a suitable antibiotic, for example the beta-lactam antibiotic imipenem, and by direct mass spectrometric measurement of the breakdown of the antibiotic by the microbial enzymes.Type: GrantFiled: April 4, 2016Date of Patent: November 19, 2019Inventors: Katrin Sparbier, Beatrix Wegemann
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Patent number: 10457972Abstract: The invention relates to a mass spectrometric method for determining microbial resistances to antibiotics. The invention provides specific methods comprising cultivation in synthetic media, in which several amino acids, preferably only a single amino acid, are isotopically labeled by incorporating 13C, 15N, 18O or 34S. If several amino acids are isotopically labeled, they are labeled in such a way that they are each heavier than the corresponding unlabeled amino acids by the same integer mass difference ?m. This ensures that the mass shifts of the peaks always amount to an integer multiple of the mass difference ?m. The total mass difference can be kept relatively small by selecting suitable amino acids. A mass shift of the protein peaks in media with antibiotics indicates that the microbes are resistant. A second embodiment first produces isotopically labeled microbes, which are then tested for their resistance by cultivating them in normal media.Type: GrantFiled: May 7, 2014Date of Patent: October 29, 2019Inventors: Jochen Franzen, Markus Kostrzewa, Christoph Lange, Katrin Sparbier
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Patent number: 10144946Abstract: The invention relates to the detection of specified, flagellated bacteria, particularly Salmonella, in food and stool. A single culturing period of about 12 to 24 hours in a liquid nutrient medium without agitation is combined with a position-selective sampling of the flagellated microbes from the liquid of the culture, after which a mass spectrometric detection method is used which recognizes the target bacteria in mixtures. A second culture step is only necessary in exceptional cases. A species-selective or genus-selective culture medium is advantageous. Positional selection becomes possible because these bacteria use their flagella to counteract sedimentation by chemotaxis, and they collect near the surface.Type: GrantFiled: May 6, 2011Date of Patent: December 4, 2018Assignee: Bruker Daltonik GmbHInventors: Katrin Sparbier, Markus Kostrzewa
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Publication number: 20180328822Abstract: The invention relates to the preparation of biological cells for the mass spectrometric analysis of cellular properties such as taxonomic classification, antibiotic resistances, response to drugs or other active substances, and others. The cells can be prokaryotic or eukaryotic microorganisms which have particularly been cultivated directly on a mass spectrometric sample support, or eukaryotic cells from tissues or cell cultures. The invention proposes that the cells are not disrupted by adding matrix solution for a subsequent ionization by matrix-assisted laser desorption (MALDI), but that they are disrupted in a separate treatment step using acids and/or solvents on the sample support itself. Surprisingly, the cell proteins released then adhere to the sample support so that they can be carefully washed with buffer solution to remove salts and other soluble impurities which can stem from earlier treatment steps, for example from nutrient solution.Type: ApplicationFiled: May 1, 2018Publication date: November 15, 2018Inventors: Katrin SPARBIER, Beatrix WEGEMANN
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Publication number: 20180312897Abstract: The invention relates to a mass-spectrometric method to determine microbial resistances to antibiotics, in which the microbes are cultured in a medium comprising an antibiotic, and a mass spectrum of the microbes is acquired after they have been cultured. The method is characterized by the fact that any microbial growth taking place during the culture is mass-spectrometrically determined with the aid of a reference substance, which is added in a dosed amount and is co-measured in the mass spectrum, wherein a growth in microbes indicates the resistance to the antibiotic.Type: ApplicationFiled: June 15, 2018Publication date: November 1, 2018Inventors: Christoph LANGE, Katrin SPARBIER
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Patent number: 10011860Abstract: The invention relates to a mass-spectrometric method to determine microbial resistances to antibiotics, in which the microbes are cultured in a medium comprising an antibiotic, and a mass spectrum of the microbes is acquired after they have been cultured. The method is characterized by the fact that any microbial growth taking place during the culture is mass-spectrometrically determined with the aid of a reference substance, which is added in a dosed amount and is co-measured in the mass spectrum, wherein a growth in microbes indicates the resistance to the antibiotic.Type: GrantFiled: April 3, 2014Date of Patent: July 3, 2018Inventors: Christoph Lange, Katrin Sparbier
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Publication number: 20160333388Abstract: The invention relates to a mass spectrometric method to determine microbial resistances to antibiotics. The decrease or modification of specific nutrient components by microbes, and thus the metabolism of the microbes, is determined mass spectrometrically in culture media containing antibiotics. Hence it is not the microbes which are introduced into the mass spectrometric analysis, but the culture medium. The special nutrient components which are subject to the mass spectrometric observation are indicators for the metabolism exhibited by the microbes in the culture in the presence of antibiotics, and are thus indicators for their susceptibility or resistance.Type: ApplicationFiled: January 14, 2015Publication date: November 17, 2016Inventors: Markus KOSTRZEWA, Katrin SPARBIER
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Publication number: 20160298164Abstract: The invention relates to methods and instruments for determining the resistances of microbes to antibiotics, in particular those microbes which produce beta-lactamases. The method determines the resistance of the microbes in less than an hour by incubating a tiny quantity of the microbes on a mass spectrometric sample support plate after they have been combined with a dosed quantity of a suitable antibiotic, for example the beta-lactam antibiotic imipenem, and by direct mass spectrometric measurement of the breakdown of the antibiotic by the microbial enzymes.Type: ApplicationFiled: April 4, 2016Publication date: October 13, 2016Inventors: Katrin SPARBIER, Beatrix WEGEMANN
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Publication number: 20160083769Abstract: The invention relates to a mass-spectrometric method to determine microbial resistances to antibiotics, in which the microbes are cultured in a medium comprising an antibiotic, and a mass spectrum of the microbes is acquired after they have been cultured. The method is characterized by the fact that any microbial growth taking place during the culture is mass-spectrometrically determined with the aid of a reference substance, which is added in a dosed amount and is co-measured in the mass spectrum, wherein a growth in microbes indicates the resistance to the antibiotic.Type: ApplicationFiled: April 3, 2014Publication date: March 24, 2016Inventors: Christoph LANGE, Katrin SPARBIER
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Publication number: 20140335556Abstract: The invention relates to a mass spectrometric method for determining microbial resistances to antibiotics. The invention provides specific methods comprising cultivation in synthetic media, in which several amino acids, preferably only a single amino acid, are isotopically labeled by incorporating 13C, 15N, 18O or 34S. If several amino acids are isotopically labeled, they are labeled in such a way that they are each heavier than the corresponding unlabeled amino acids by the same integer mass difference ?m. This ensures that the mass shifts of the peaks always amount to an integer multiple of the mass difference ?m. The total mass difference can be kept relatively small by selecting suitable amino acids. A mass shift of the protein peaks in media with antibiotics indicates that the microbes are resistant. A second embodiment first produces isotopically labeled microbes, which are then tested for their resistance by cultivating them in normal media.Type: ApplicationFiled: May 7, 2014Publication date: November 13, 2014Inventors: Jochen FRANZEN, Markus KOSTRZEWA, Christoph LANGE, Katrin SPARBIER
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Publication number: 20130095511Abstract: The invention relates to the determination of resistances of microorganisms which produce ?-lactamases, in particular “extended spectrum ?-lactamases” (ESBL). The invention provides a method whereby the microbial resistance can be measured very simply and quickly by means of the catalytic effect of the microbially produced ?-lactamases on ?-lactam antibiotics, which consists in a hydrolytic cleavage of the ?-lactam ring. The method determines the resistance of the bacteria a few hours after a suitable substrate, either a ?-lactam antibiotic or a customized ?-lactam derivative, has been added to a suspension of the microbes, by direct mass spectrometric measurement of the substrate breakdown caused by the ?-lactamases.Type: ApplicationFiled: June 10, 2011Publication date: April 18, 2013Inventors: Markus Kostrzewa, Karsten Michelmann, Katrin Sparbier
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Publication number: 20120107864Abstract: The invention relates to the detection of specified, flagellated bacteria, particularly Salmonella, in food and stool. A single culturing period of about 12 to 24 hours in a liquid nutrient medium without agitation is combined with a position-selective sampling of the flagellated microbes from the liquid of the culture, after which a mass spectrometric detection method is used which recognizes the target bacteria in mixtures. A second culture step is only necessary in exceptional cases. A species-selective or genus-selective culture medium is advantageous. Positional selection becomes possible because these bacteria use their flagella to counteract sedimentation by chemotaxis, and they collect near the surface.Type: ApplicationFiled: May 6, 2011Publication date: May 3, 2012Inventors: Katrin Sparbier, Markus Kostrzewa