Abstract: A polylactic acid (PLA) multi-layer film is produced by arranging a flexible biodegradable polyester resin having a modulus of elasticity of 50 to 1,000 MPa between two kinds of polylactic acids and co-extruding the three through a die. The multi-layer film of the invention is constituted of an interlayer of a biodegradable polyester resin, which is opaque and has blocking tendency though it is very flexible, and transparent and hard PLA layers between which the interlayer is sandwiched, so that the multi-layer film is improved in wrinkle, surface waviness, and elongation and impact resistance of film in which a film made of PLA alone is problematic-by virtue of the presence of the biodegradable polyester resin layer, and in transparency and blocking resistance-in which a film made of a biodegradable polyester resin alone is problematic-by virtue of the PLA layers.