Abstract: A substrate for feeding cells and/or tissues which is composed of a porous body having a thin film surface and a porous surface and which can be used in the regenerative medical techniques. A cell/tissue feeder is produced by cultivating tissues on the porous surface of the porous body. Cells are seeded on the porous surface of the body, followed by the cultivation of the cells to thus form and/or regenerate tissues. An aqueous solution of silk proteins, to which a water-soluble organic solvent is added, is introduced into a mold whose bottom or top surface is surface-roughened, followed by freezing the aqueous solution and then thawing the frozen aqueous solution to form a porous body having a thin film surface and a porous surface.

Abstract: A substrate for feeding cells and/or tissues which is composed of a porous body having a thin film surface and a porous surface and which can be used in the regenerative medical techniques. A cell/tissue feeder is produced by cultivating tissues on the porous surface of the porous body. Cells are seeded on the porous surface of the body, followed by the cultivation of the cells to thus form and/or regenerate tissues. An aqueous solution of silk proteins, to which a water-soluble organic solvent is added, is introduced into a mold whose bottom or top surface is surface-roughened, followed by freezing the aqueous solution and then thawing the frozen aqueous solution to form a porous body having a thin film surface and a porous surface.

Abstract: In this invention, the charging units are in the separate construction for the main chargers and for the ozone ducts. When installing the process units, the ozone ducts are placed on the elastic sheets of the duct cases that are fixed to the main frame. The process units are slid while slanting the ozone ducts to the duct cases by the main chargers against the bias force of the elastic sheets. Fluctuation in manufacturing accuracy is absorbed by the elastic sheets and the maintenance efficiency when installing/removing the process units is improved by sliding the process units smoothly. Further, the charging units are supported certainly by the main frame.

Abstract: In this invention, the charging units are in the separate construction for the main chargers and for the ozone ducts. When installing the process units, the ozone ducts are placed on the elastic sheets of the duct cases that are fixed to the main frame. The process units are slid while slanting the ozone ducts to the duct cases by the main chargers against the bias force of the elastic sheets. Fluctuation in manufacturing accuracy is absorbed by the elastic sheets and the maintenance efficiency when installing/removing the process units is improved by sliding the process units smoothly. Further, the charging units are supported certainly by the main frame.

Abstract: In this invention, the charging units are in the separate construction for the main chargers and for the ozone ducts. When installing the process units, the ozone ducts are placed on the elastic sheets of the duct cases that are fixed to the main frame. The process units are slid while slanting the ozone ducts to the duct cases by the main chargers against the bias force of the elastic sheets. Fluctuation in manufacturing accuracy is absorbed by the elastic sheets and the maintenance efficiency when installing/removing the process units is improved by sliding the process units smoothly. Further, the charging units are supported certainly by the main frame.

Abstract: Transgenic silkworms comprising GFP whose expression is regulated by the sericin gene promoter were produced. Observation of the silk glands of the last instar larvae of the silkworms showed fluorescence only in the middle silk glands. GFP was secreted from middle silkgland cells from around the spinning stage, indicating that GFP moved into the gland lumen. Finally, GFP was spun into cocoon filaments, and cocoons containing large amounts of GFP were produced. Thus, by using the promoter region of the sericin gene, recombinant proteins can be produced in the middle silk glands. Furthermore, the recombinant proteins produced in the middle silk glands were readily secreted into the lumen of the middle silk glands.

Abstract: In this invention, the charging units are in the separate construction for the main chargers and for the ozone ducts. When installing the process units, the ozone ducts are placed on the elastic sheets of the duct cases that are fixed to the main frame. The process units are slid while slanting the ozone ducts to the duct cases by the main chargers against the bias force of the elastic sheets. Fluctuation in manufacturing accuracy is absorbed by the elastic sheets and the maintenance efficiency when installing/removing the process units is improved by sliding the process units smoothly. Further, the charging units are supported certainly by the main frame.

Abstract: Transgenic silkworms comprising GFP whose expression is regulated by the sericin gene promoter were produced. Observation of the silk glands of the last instar larvae of the silkworms showed fluorescence only in the middle silk glands. GFP was secreted from middle silkgland cells from around the spinning stage, indicating that GFP moved into the gland lumen. Finally, GFP was spun into cocoon filaments, and cocoons containing large amounts of GFP were produced. Thus, by using the promoter region of the sericin gene, recombinant proteins can be produced in the middle silk glands. Furthermore, the recombinant proteins produced in the middle silk glands were readily secreted into the lumen of the middle silk glands.

Abstract: A developing apparatus includes a housing having a toner holding space and toner discharging aperature, a developing roller rotated in a predetermined direction in the aperature, a toner leveling member closing a gap between a periphery of the developing roller and an edge of the aperture at which the periphery of the developing roller is moved out from the aperture in its predetermined rotating direction, and slidably pressed against the periphery of the developing roller, a toner supplying roller rotated in the space and pressed against the periphery of the developing roller, and a sliding and closing member closing a gap between the periphery of the supplying roller and a portion of the inner surface of the housing corresponding to a gap between the periphery of the developing roller and an edge of the aperture at which the periphery of the developing roller is moved into the aperature in its predetermined rotating direction, and slidably pressed against the periphery of the supplying roller.