Abstract: The present invention is a method for purifying an enzyme, particularly an acylase, comprising causing selective aggregation and precipitation of a contaminant enzyme, particularly deacetylase, by the use of a surfactant, particularly a cationic surfactant. The present invention is also a method for regenerating an immobilized enzyme carrier, particularly a synthetic adsorbent or ion exchange resin, comprising allowing a protease to act on the immobilized enzyme to remove the enzyme from the carrier, said immobilized enzyme being prepared by binding the enzyme with the carrier and optionally crosslinking the enzymes by the use of a crosslinking agent after binding. Particularly, the present invention is the above-mentioned carrier regeneration method, wherein the carrier has fine pores, and the above-mentioned carrier regeneration method, wherein the enzyme is cephalosporin C acylase.

Abstract: An enzyme in a mixture containing the enzyme and a contaminant enzyme is purified by selectively aggregating and precipitating the contaminant enzyme with a surfactant. A deacetylase contaminant is separated from Cephalosporin C acylase using a cationic surfactant in an amount of 0.1 to 0.6%. Surfactants include benzylated or methylated cationic surfactants such as alkyl(palm)dimethylbenzyl ammonium chloride, alkyl(palm)trimethyl ammonium chloride and dodecyltrimethyl ammonium chloride. An immobilized enzyme is regenerated using a protease to remove the enzyme from a carrier. The carrier may be a synthetic adsorbent or an ion exchange resin having pores of 100 nm or less in diameter, and the immobilized enzyme is optionally crosslinked. Immobilized cephalosporin C acylase is regenerated using an alkaline or acidic protease.

Abstract: The present invention is a method for purifying an enzyme, particularly an acylase, comprising causing selective aggregation and precipitation of a contaminant enzyme, particularly deacetylase, by the use of a surfactant, particularly a cationic surfactant. The present invention is also a method for regenerating an immobilized enzyme carrier, particularly a synthetic adsorbent or ion exchange resin, comprising allowing a protease to act on the immobilized enzyme to remove the enzyme from the carrier, said immobilized enzyme being prepared by binding the enzyme with the carrier and optionally crosslinking the enzymes by the use of a crosslinking agent after binding. Particularly, the present invention is the above-mentioned carrier regeneration method, wherein the carrier has fine pores, and the above-mentioned carrier regeneration method, wherein the enzyme is cephalosporin C acylase.

Abstract: An enzyme in a mixture containing the enzyme and a contaminant enzyme is purified by selectively aggregating and precipitating the contaminant enzyme with a surfactant. A carrier containing an immobilized enzyme is regenerated by using a protease to remove the enzyme from the carrier. Cephalosporin C acylase is purified from a mixture of the acylase and a deacetylase contaminant by selectively aggregating and precipitating the deacetylase contaminant by adding to the solution a benzylated cationic or methylated cationic surfactant in an amount of 0.1 to 0.6% of the mixture. The surfactant may be alkyl(palm)dimethylbenzyl ammonium chloride. A carrier containing immobilized cephalosporin C acylase is regenerated by contacting the carrier with an alkaline or acidic protease. The acylase is immobilized by being bound to the carrier and optionally crosslinked. The carrier may have pores of 100 nm or less in diameter.