Patents by Inventor Katsutoshi Ara
Katsutoshi Ara has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9029519Abstract: The present invention provides a modified promoter, an expression vector and a transformant each containing the promoter, and a method for producing a gene product of interest using the transformant. The invention provides a modified promoter, including a nucleotide sequence of a promoter derived from bacterium belonging to the genus Bacillus in which at least one nucleotide sequence selected from the following has been modified: a nucleotide sequence represented by SEQ ID NO: 1; a nucleotide sequence equivalent to the nucleotide sequence represented by SEQ ID NO: 1, except that one or a plurality of bases therein are substituted, deleted, added or inserted; and a nucleotide sequence having a sequence identity of 70% or more with respect to the nucleotide sequence represented by SEQ ID NO: 1.Type: GrantFiled: October 18, 2010Date of Patent: May 12, 2015Assignee: Kao CorporationInventors: Akihito Kawahara, Hiroshi Kodama, Katsutoshi Ara
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Patent number: 8852943Abstract: A method of modifying a target region in a host DNA using a donor DNA: wherein the donor DNA having regions homologous to a 5?-side region outside of the target region in the host DNA, a 3?-side region outside of the target region in the host DNA and a first homologous recombination region inside of the target region in the host DNA, respectively, in this order, and further having a first selectable marker gene, an expression-inducing promoter and a second selectable marker gene expressed under the control of the expression-inducing promoter between the region homologous to the 3?-side region and the region homologous to the first homologous recombination region; which method has the steps of: a first step of performing homologous recombination between the donor DNA and the host DNA at the regions of the 5?-side region and the first homologous recombination region, to conduct selection of a host integrated with the donor DNA based on expression of the first selectable marker gene; and a second step of performType: GrantFiled: March 23, 2009Date of Patent: October 7, 2014Assignee: Kao CorporationInventors: Katsutoshi Ara, Takuya Morimoto, Naotake Ogasawara
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Patent number: 8790726Abstract: Deodorant agents, which contain ginkgo or Phellodendron Bark or its extract as an active ingredient and which are high in safety and can radically inhibit the emission of human body malodors typified by sweat odor, especially, axillary odor.Type: GrantFiled: August 27, 2009Date of Patent: July 29, 2014Assignee: Kao CorporationInventors: Syunichi Akiba, Katsutoshi Ara, Hiroshi Kusuoku, Toyoki Hagura
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Publication number: 20130295676Abstract: A method of modifying a target region in a host DNA using a donor DNA: wherein the donor DNA having regions homologous to a 5?-side region outside of the target region in the host DNA, a 3?-side region outside of the target region in the host DNA and a first homologous recombination region inside of the target region in the host DNA, respectively, in this order, and further having a first selectable marker gene, an expression-inducing promoter and a second selectable marker gene expressed under the control of the expression-inducing promoter between the region homologous to the 3?-side region and the region homologous to the first homologous recombination region; which method has the steps of: a first step of performing homologous recombination between the donor DNA and the host DNA at the regions of the 5?-side region and the first homologous recombination region, to conduct selection of a host integrated with the donor DNA based on expression of the first selectable marker gene; and a second step of performType: ApplicationFiled: March 23, 2009Publication date: November 7, 2013Applicant: Kao CorporationInventors: Katsutoshi ARA, Takuya MORIMOTO, Naotake OGASAWARA
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Patent number: 8460893Abstract: The object of the invention is to provide a microorganism which enhances productivity of cellulase and is useful for industrial production of cellulase, and to provide a method for producing cellulase by use of the microorganism. The present invention provides a recombinant microorganism produced by transferring a gene encoding cellulase to a parental microorganism which has been genetically modified so as to overexpress a Bacillus subtilis secY gene or a gene corresponding thereto.Type: GrantFiled: February 15, 2007Date of Patent: June 11, 2013Assignee: Kao CorporationInventors: Keiji Endo, Shenghao Liu, Katsutoshi Ara
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Patent number: 8389264Abstract: A recombinant microorganism having improved productivity for a protein or a polypeptide, and a method for producing a protein or a polypeptide using the recombinant microorganism, are provided. A recombinant microorganism obtained by transfecting a gene for encoding a desired protein or polypeptide into a microorganism strain which is obtained by genetically constructing to overexpress secY gene of Bacillus subtilis or a gene corresponding to the secY gene, and deleting or inactivating one or more genes selected from sporulation-associated genes and genes corresponding to the sporulation-associated genes from the genome.Type: GrantFiled: April 8, 2008Date of Patent: March 5, 2013Assignee: Kao CorporationInventors: Shenghao Liu, Keiji Endo, Katsutoshi Ara
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Patent number: 8293516Abstract: A recombinant microorganism is provided that has increased productivity of a protein or polypeptide of interest. A method for producing a protein of interest using the microorganism is also provided. The microorganism is prepared by inserting, in its genome, a transcription initiation regulatory region or both a transcription initiation regulatory region and a ribosome-binding site, upstream of a Bacillus subtilis prsA gene and by deleting or inactivating one or more of the abrB gene, dltA gene, dltB gene, dltC gene, dltD gene, dltE gene, or a gene (genes) corresponding thereto.Type: GrantFiled: December 27, 2007Date of Patent: October 23, 2012Assignee: Kao CorporationInventors: Keiji Endo, Katsutoshi Ara
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Patent number: 8236524Abstract: To provide a microorganism with enhanced secretory production of a protein or polypeptide and a method of producing the protein or polypeptide using the microorganism. A modified microorganism that has been genetically modified to delete 60 to 80 carboxyl-terminal amino acids of SecA.Type: GrantFiled: April 1, 2008Date of Patent: August 7, 2012Assignee: Kao CorporationInventors: Katsutoshi Ara, Hiroshi Kakeshita, Kouji Nakamura
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Publication number: 20120183998Abstract: The present invention provides a modified promoter, an expression vector and a transformant each containing the promoter, and a method for producing a gene product of interest using the transformant. The invention provides a modified promoter, including a nucleotide sequence of a promoter derived from bacterium belonging to the genus Bacillus in which at least one nucleotide sequence selected from the following has been modified: a nucleotide sequence represented by SEQ ID NO: 1; a nucleotide sequence equivalent to the nucleotide sequence represented by SEQ ID NO: 1, except that one or a plurality of bases therein are substituted, deleted, added or inserted; and a nucleotide sequence having a sequence identity of 70% or more with respect to the nucleotide sequence represented by SEQ ID NO: 1.Type: ApplicationFiled: October 18, 2010Publication date: July 19, 2012Applicant: Kao CorporationInventors: Akihito Kawahara, Hiroshi Kodama, Katsutoshi Ara
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Publication number: 20110275158Abstract: A method of modifying a target region in a host DNA using a donor DNA: wherein the donor DNA having regions homologous to a 5?-side region outside of the target region in the host DNA, a 3?-side region outside of the target region in the host DNA and a first homologous recombination region inside of the target region in the host DNA, respectively, in this order, and further having a first selectable marker gene, an expression-inducing promoter and a second selectable marker gene expressed under the control of the expression-inducing promoter between the region homologous to the 3?-side region and the region homologous to the first homologous recombination region; which method has the steps of: a first step of performing homologous recombination between the donor DNA and the host DNA at the regions of the 5?-side region and the first homologous recombination region, to conduct selection of a host integrated with the donor DNA based on expression of the first selectable marker gene; and a second step of performType: ApplicationFiled: October 4, 2010Publication date: November 10, 2011Applicant: Kao CorporationInventors: Katsutoshi ARA, Takuya MORIMOTO, Naotake OGASAWARA
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Patent number: 7981659Abstract: Novel Bacillus subtilis mutant strains having good productivity of various enzymes are provided through extensive analysis of strains that are derived from Bacillus subtilis via gene disruption. The Bacillus subtilis mutant strains according to the present invention have genomic structures prepared by deletion of regions listed in the columns for deficient regions. Each of these Bacillus subtilis mutant strains exerts significantly improved secretory productivity of a protein when a gene encoding such a secretory target protein is introduced so that it can be expressed, compared with a case in which the same gene is introduced into a wild-type strain.Type: GrantFiled: September 25, 2006Date of Patent: July 19, 2011Assignees: Kao Corporation, Nara Institute of Science and TechnologyInventors: Ryosuke Kadoya, Keiji Endo, Masatoshi Tohata, Katsutoshi Ara, Naotake Ogasawara
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Publication number: 20110151567Abstract: A recombinant microorganism having improved productivity for a protein or a polypeptide, and a method for producing a protein or a polypeptide using the recombinant microorganism, are provided. A recombinant microorganism obtained by transfecting a gene for encoding a desired protein or polypeptide into a microorganism strain which is obtained by genetically constructing to overexpress secY gene of Bacillus subtilis or a gene corresponding to the secY gene, and deleting or inactivating one or more genes selected from sporulation-associated genes and genes corresponding to the sporulation-associated genes from the genome.Type: ApplicationFiled: April 8, 2008Publication date: June 23, 2011Applicant: KAO CORPORATIONInventors: Shenghao Liu, Keiji Endo, Katsutoshi Ara
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Publication number: 20110014709Abstract: A method of modifying a target region in a host DNA using a donor DNA: wherein the donor DNA having regions homologous to a 5?-side region outside of the target region in the host DNA, a 3?-side region outside of the target region in the host DNA and a first homologous recombination region inside of the target region in the host DNA, respectively, in this order, and further having a first selectable marker gene, an expression-inducing promoter and a second selectable marker gene expressed under the control of the expression-inducing promoter between the region homologous to the 3?-side region and the region homologous to the first homologous recombination region; which method has the steps of: a first step of performing homologous recombination between the donor DNA and the host DNA at the regions of the 5?-side region and the first homologous recombination region, to conduct selection of a host integrated with the donor DNA based on expression of the first selectable marker gene; and a second step of pType: ApplicationFiled: March 23, 2009Publication date: January 20, 2011Applicant: Kao CorporationInventors: Katsutoshi Ara, Takuya Morimoto, Naotake Ogasawara
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Publication number: 20100297703Abstract: This invention relates to inhibition of microbial lysis via regulation of activity of a lytic enzyme for degrading the cell wall. The lytic enzyme inhibitor and the lysis inhibitor are each composed mainly of the Bacillus subtilis-derived YoeB gene or the YoeB protein encoded by a gene homologous to the YoeB gene. An example of the YoeB protein is a protein consisting of the amino acid sequence as shown in SEQ ID NO: 2.Type: ApplicationFiled: October 16, 2007Publication date: November 25, 2010Inventors: Katsutoshi Ara, Junichi Sekiguchi, Hiroki Yamamoto, Hiroyuki Harada
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Publication number: 20100255534Abstract: Provision of a recombinant microorganism which has increased productivity of a protein or polypeptide of interest and a method for producing a protein or polypeptide of interest using the recombinant microorganism.Type: ApplicationFiled: December 27, 2007Publication date: October 7, 2010Applicant: KAO CORPORATIONInventors: Keiji Endo, Katsutoshi Ara
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Publication number: 20100093032Abstract: To provide a microorganism with enhanced secretory production of a protein or polypeptide and a method of producing the protein or polypeptide using the microorganism. A modified microorganism that has been genetically modified to delete 60 to 80 carboxyl-terminal amino acids of SecA.Type: ApplicationFiled: April 1, 2008Publication date: April 15, 2010Inventors: Katsutoshi Ara, Hiroshi Kakeshita, Kouji Nakamura
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Publication number: 20090317346Abstract: This invention relates to deodorant agents, which contain ginkgo or Phellodendron Bark or its extract as an active ingredient. According to the present invention, deodorant agents which are high in safety and can radically inhibit the emission of human body malodors typified by sweat odor, especially, axillary odor.Type: ApplicationFiled: August 27, 2009Publication date: December 24, 2009Applicant: Kao CorporationInventors: Syunichi Akiba, Katsutoshi Ara, Hiroshi Kusuoku, Toyoki Hagura
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Publication number: 20090221055Abstract: Novel Bacillus subtilis mutant strains having good productivity of various enzymes are provided through extensive analysis of strains that are derived from Bacillus subtilis via gene disruption. The Bacillus subtilis mutant strains according to the present invention have genomic structures prepared by deletion of regions listed in the columns for deficient regions. Each of these Bacillus subtilis mutant strains exerts significantly improved secretory productivity of a protein when a gene encoding such a secretory target protein is introduced so that it can be expressed, compared with a case in which the same gene is introduced into a wild-type strain.Type: ApplicationFiled: September 25, 2006Publication date: September 3, 2009Inventors: Ryosuke Kadoya, Keiji Endo, Masatoshi Tohata, Katsutoshi Ara, Naotake Ogasawara
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Publication number: 20090029417Abstract: The object of the invention is to provide a microorganism which enhances productivity of cellulase and is useful for industrial production of cellulase, and to provide a method for producing cellulase by use of the microorganism. The present invention provides a recombinant microorganism produced by transferring a gene encoding cellulase to a parental microorganism which has been genetically modified so as to overexpress a Bacillus subtilis secY gene or a gene corresponding thereto.Type: ApplicationFiled: February 15, 2007Publication date: January 29, 2009Applicant: Kao CorporationInventors: Keiji Endo, Shenghao Liu, Katsutoshi Ara
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Publication number: 20080050807Abstract: The present invention provides a DNA fragment encoding alkaline liquefying ?-amylase, recombinant DNA containing the DNA fragment, a transformed microorganism harboring the recombinant DNA, as well as a method for producing alkaline liquefying ?-amylase using the transformant. The method of the present invention enables mass production of alkaline liquefying ?-amylase useful as a detergent component. The present invention is directed to a liquefying alkaline alph?-amylase, and a DNA encoding for the same and functional fragments thereof.Type: ApplicationFiled: April 22, 2004Publication date: February 28, 2008Inventors: Yuji Hatada, Katsuya Ozaki, Katsutoshi Ara, Shuji Kawai, Susumu Ito