Patents by Inventor Katsuya Ozaki
Katsuya Ozaki has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20140170703Abstract: A recombinant microorganism obtained by transferring, into a host microorganism capable of producing protein or polypeptide with increased productivity, a gene encoding a protein or polypeptide, and a method for producing a protein or polypeptide by use of the recombinant microorganism. The recombinant microorganism is prepared by transferring, to a mutant strain of microorganism from which any of Bacillus subtilis genes comA, yopO, treR, yvbA, cspB, yvaN, yttP, yurK, yozA, licR, sigL, mntR, glcT, yvdE, ykvE, slr, rocR, ccpA, yaaT, yyaA, yycH, yacP, hprK, rsiX, yhdK, and ylbO, or one or more genes functionally equivalent to any of these genes have been deleted or knocked out, a gene encoding a heterologous protein or polypeptide.Type: ApplicationFiled: November 25, 2013Publication date: June 19, 2014Applicant: KAO CORPORATIONInventors: MASATOSHI TOHATA, KAZUHISA SAWADA, KATSUYA OZAKI, KAZUO KOBAYASHI, NAOTAKE OGASAWARA
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Patent number: 8623631Abstract: The present invention provides a modified promoter DNA capable of enhancing transcription of genes encoding proteins or polypeptides, and a method for producing proteins or polypeptides efficiently by use of the modified promoter DNA. A promoter DNA recognized by SigA and SigE, which is produced by modifying a nucleotide sequence including a promoter recognized by SigA and bases in the vicinity thereof; an expression vector harboring the promoter DNA; a recombinant microorganism containing the expression vector; and a method for producing proteins or polypeptides characterized by culturing the recombinant microorganism.Type: GrantFiled: March 4, 2005Date of Patent: January 7, 2014Assignee: Kao CorporationInventors: Keiji Endo, Katsuya Ozaki
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Patent number: 7855065Abstract: The present invention provides a mutant Bacillus bacterium capable of enhancing productivity of proteins or polypeptides, a recombinant microorganism produced by introducing genes encoding heterologous proteins or polypeptides into the mutant Bacillus bacterium, and a method for producing proteins or polypeptides by use of the recombinant microorganism. A mutant Bacillus bacterium which has, on the genome or plasmid thereof, DNA having a promoter sequence which is recognized and transcribed specifically during the sporulation stage and which is ligated to an upstream end of a sigA gene or a gene equivalent thereto, a recombinant microorganism produced by introducing genes encoding heterologous proteins or polypeptides into the mutant Bacillus bacterium, and a method for producing proteins or polypeptides by use of the recombinant microorganism.Type: GrantFiled: March 4, 2005Date of Patent: December 21, 2010Assignee: Kao CorporationInventors: Keiji Endo, Katsuya Ozaki
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Patent number: 7829322Abstract: A host microorganism capable of increasing productivity of a protein or polypeptide, a recombinant microorganism obtained by introducing a gene encoding a protein or polypeptide into the host microorganism, and a method for producing a protein or polypeptide using the recombinant microorganism are provided. Also provided is a recombinant microorganism obtained by introducing into a host microorganism a gene encoding a heterologous protein or polypeptide, wherein in said host microorganism the Bacillus subtilis aprX gene or a gene corresponding to the aprX gene has been deleted or knocked out, and a method for producing a protein or polypeptide using the recombinant microorganism.Type: GrantFiled: December 20, 2005Date of Patent: November 9, 2010Assignees: Kao Corporation, Shinshu UniversityInventors: Takeko Kodama, Keiji Endo, Katsuya Ozaki, Junichi Sekiguchi
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Publication number: 20090226961Abstract: The invention relates to a mutant ?-amylase obtained by making replacement or deletion of at least one of amino acid residues such as the 167th Gln, 169th Tyr and 178th Ala in the amino acid sequence set forth in SEQ ID NO:1 in an ?-amylase having said amino acid sequence, or an ?-amylase having a homology of at least 70% to said amino acid sequence, a gene encoding the mutant ?-amylase, a vector, transformed cells, a process for producing a mutant ?-amylase, comprising culturing the transformed cells, and a detergent composition comprising the mutant ?-amylase. The mutant ?-amylase of the invention has excellent properties of high resistance to chelating agents, high specific activity in an alkaline region and excellent stability to heat, and is hence useful for detergents for automatic dish washer, laundry detergents and the like.Type: ApplicationFiled: September 28, 2007Publication date: September 10, 2009Inventors: Keiji Endo, Kazuaki Igarashi, Yasuhiro Hayashi, Hiroshi Hagihara, Katsuya Ozaki
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Patent number: 7585674Abstract: A microorganism wherein one or more genes selected from the group of genes participating in sporulation in the middle to late stages of sporulation have been deleted or inactivated; and a process for producing a target product (a protein) by use the microorganism. No spore is formed when this microorganism is employed, thereby enabling production of a target product (a protein) while decreasing energy loss, production of a by-product and specific production speed to decrease unnecessary consumption of a medium. Moreover, the production period can be prolonged, whereby the target product (the protein) can be produced efficiently.Type: GrantFiled: May 28, 2002Date of Patent: September 8, 2009Assignee: Kao CorporationInventors: Kazuhisa Sawada, Keiji Endo, Tadahiro Ozawa, Masatoshi Tohata, Katsuya Ozaki
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Patent number: 7563611Abstract: The present invention provides a recombinant microorganism obtained by transferring, into a host microorganism which is capable of producing protein or polypeptide with increased productivity and which was identified by the present inventors, a gene encoding a protein or polypeptide, and a method for producing a protein or polypeptide by use of the recombinant microorganism.Type: GrantFiled: November 5, 2004Date of Patent: July 21, 2009Assignee: Kao CorporationInventors: Masatoshi Tohata, Kazuhisa Sawada, Katsuya Ozaki, Junichi Sekiguchi
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Publication number: 20090170154Abstract: The present invention provides a mutant Bacillus bacterium capable of enhancing productivity of proteins or polypeptides, a recombinant microorganism produced by introducing genes encoding heterologous proteins or polypeptides into the mutant Bacillus bacterium, and a method for producing proteins or polypeptides by use of the recombinant microorganism. A mutant Bacillus bacterium which has, on the genome or plasmid thereof, DNA having a promoter sequence which is recognized and transcribed specifically during the sporulation stage and which is ligated to an upstream end of a sigA gene or a gene equivalent thereto, a recombinant microorganism produced by introducing genes encoding heterologous proteins or polypeptides into the mutant Bacillus bacterium, and a method for producing proteins or polypeptides by use of the recombinant microorganism.Type: ApplicationFiled: March 4, 2005Publication date: July 2, 2009Applicant: KAO CORPORATIONInventors: Keiji Endo, Katsuya Ozaki
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Publication number: 20090081726Abstract: A host microorganism capable of increasing productivity of a protein or polypeptide, a recombinant microorganism obtained by introducing a gene encoding a protein or polypeptide into the host microorganism, and a method for producing a protein or polypeptide using the recombinant microorganism are provided. Also provided is a recombinant microorganism obtained by introducing into a host microorganism a gene encoding a heterologous protein or polypeptide, wherein in said host microorganism the Bacillus subtilis aprX gene or a gene corresponding to the aprX gene has been deleted or knocked out, and a method for producing a protein or polypeptide using the recombinant microorganism.Type: ApplicationFiled: December 20, 2005Publication date: March 26, 2009Inventors: Takeko Kodama, Keiji Endo, Katsuya Ozaki, Junichi Sekiguchi
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Publication number: 20080081352Abstract: The present invention provides a recombinant microorganism obtained by transferring, into a host microorganism which is capable of producing protein or polypeptide with increased productivity and which was identified by the present inventors, a gene encoding a protein or polypeptide, and a method for producing a protein or polypeptide by use of the recombinant microorganism. The recombinant microorganism is prepared by transferring, to a mutant strain of microorganism from which any of at least one gene participating in membrane permeation of maltose has been deleted or knocked out, a gene encoding a heterologous protein or polypeptide.Type: ApplicationFiled: November 5, 2004Publication date: April 3, 2008Applicant: Kao CorporationInventors: Masatoshi Tohata, Kazuhisa Sawada, Katsuya Ozaki, Junichi Sekiguchi
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Publication number: 20080050807Abstract: The present invention provides a DNA fragment encoding alkaline liquefying ?-amylase, recombinant DNA containing the DNA fragment, a transformed microorganism harboring the recombinant DNA, as well as a method for producing alkaline liquefying ?-amylase using the transformant. The method of the present invention enables mass production of alkaline liquefying ?-amylase useful as a detergent component. The present invention is directed to a liquefying alkaline alph?-amylase, and a DNA encoding for the same and functional fragments thereof.Type: ApplicationFiled: April 22, 2004Publication date: February 28, 2008Inventors: Yuji Hatada, Katsuya Ozaki, Katsutoshi Ara, Shuji Kawai, Susumu Ito
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Publication number: 20080026976Abstract: The invention relates to mutant ?-amylases that may be produced at high yield from recombinant microorganisms.Type: ApplicationFiled: November 1, 2006Publication date: January 31, 2008Applicant: Kao CorporationInventors: Hiroyuki Araki, Keiji Endo, Hiroshi Hagihara, Kazuaki Igarashi, Yasuhiro Hayashi, Katsuya Ozaki
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Publication number: 20080014608Abstract: The present invention provides a modified promoter DNA capable of enhancing transcription of genes encoding proteins or polypeptides, and a method for producing proteins or polypeptides efficiently by use of the modified promoter DNA. A promoter DNA recognized by SigA and SigE, which is produced by modifying a nucleotide sequence including a promoter recognized by SigA and bases in the vicinity thereof; an expression vector harboring the promoter DNA; a recombinant microorganism containing the expression vector; and a method for producing proteins or polypeptides characterized by culturing the recombinant microorganism.Type: ApplicationFiled: March 4, 2005Publication date: January 17, 2008Applicant: KAO CORPORATIONInventors: Keiji Endo, Katsuya Ozaki
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Publication number: 20080009039Abstract: A recombinant microorganism obtained by transferring, into a host microorganism capable of producing protein or polypeptide with increased productivity, a gene encoding a protein or polypeptide, and a method for producing a protein or polypeptide by use of the recombinant microorganism. The recombinant microorganism is prepared by transferring, to a mutant strain of microorganism from which any of Bacillus subtilis genes comA, yopO, treR, yvbA, cspB, yvaN, yttP, yurK, yozA, licR, sigL, mntR, glcT, yvdE, ykvE, sir, rocR, ccpA, yaaT, yyaA, yycH, yacP, hprK, rsiX, yhdK, and yibO, or one or more genes functionally equivalent to any of these genes have been deleted or knocked out, a gene encoding a heterologous protein or polypeptide.Type: ApplicationFiled: November 5, 2004Publication date: January 10, 2008Applicant: Kao CorporationInventors: Masatoshi Tohata, Kazuhisa Sawada, Katsuya Ozaki, Kazuo Kobayashi, Naotake Ogasawara
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Patent number: 7297527Abstract: The invention relates to mutant ?-amylases that may be produced at high yield from recombinant microorganisms.Type: GrantFiled: March 12, 2004Date of Patent: November 20, 2007Assignee: Kao CorporationInventors: Hiroyuki Araki, Keiji Endo, Hiroshi Hagihara, Kazuaki Igarashi, Yasuhiro Hayashi, Katsuya Ozaki
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Patent number: 7078212Abstract: The invention relates to a mutant ?-amylase obtained by making replacement or deletion of at least one of amino acid residues such as the 167th Gln, 169th Tyr and 178th Ala in the amino acid sequence set forth in SEQ ID NO:1 in an ?-amylase having said amino acid sequence, or an ?-amylase having a homology of at least 70% to said amino acid sequence, a gene encoding the mutant ?-amylase, a vector, transformed cells, a process for producing a mutant ?-amylase, comprising culturing the transformed cells, and a detergent composition comprising the mutant ?-amylase. The mutant ?-amylase of the invention has excellent properties of high resistance to chelating agents, high specific activity in an alkaline region and excellent stability to heat, and is hence useful for detergents for automatic dish washer, laundry detergents and the like.Type: GrantFiled: June 9, 2000Date of Patent: July 18, 2006Assignee: Kao CorporationInventors: Keiji Endo, Kazuaki Igarashi, Yasuhiro Hayashi, Hiroshi Hagihara, Katsuya Ozaki
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Patent number: 6979731Abstract: The present invention is directed to a liquefying alkaline alpha-amylase, and a DNA encoding for the same and functional fragments thereof.Type: GrantFiled: June 14, 1996Date of Patent: December 27, 2005Assignee: Kao CorporationInventors: Yuji Hatada, Katsuya Ozaki, Katsutoshi Ara, Shuji Kawai, Susumu Ito
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Publication number: 20050181493Abstract: The invention relates to a mutant ?-amylase obtained by making replacement or deletion of at least one of amino acid residues such as the 167th Gln, 169th Tyr and 178th Ala in the amino acid sequence set forth in SEQ ID NO:1 in an ?-amylase having said amino acid sequence, or an ?-amylase having a homology of at least 70% to said amino acid sequence, a gene encoding the mutant ?-amylase, a vector, transformed cells, a process for producing a mutant ?-amylase, comprising culturing the transformed cells, and a detergent composition comprising the mutant ?-amylase. The mutant ?-amylase of the invention has excellent properties of high resistance to chelating agents, high specific activity in an alkaline region and excellent stability to heat, and is hence useful for detergents for automatic dish washer, laundry detergents and the like.Type: ApplicationFiled: November 16, 2004Publication date: August 18, 2005Inventors: Keiji Endo, Kazuaki Igarashi, Yasuhiro Hayashi, Hiroshi Hagihara, Katsuya Ozaki
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Patent number: 6916645Abstract: Liquefying alkaline amylases, each having residual activity not less than 70% when treated at pH 10 and 45° C. for 30 minutes in the presence of 1 to 100 mM of EDTA or EGTA, are described. Also described are detergents comprising these amylases. In comparison with conventional amylases for detergents, the liquefying alkaline amylases of this invention have a high chelating-agent resisting performance.Type: GrantFiled: May 2, 2002Date of Patent: July 12, 2005Assignee: Kao CorporationInventors: Hiroshi Hagihara, Kaori Kitayama, Yasuhiro Hayashi, Kazuaki Igarashi, Keiji Endo, Katsuya Ozaki
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Publication number: 20040265959Abstract: The invention relates to mutant &agr;-amylases that may be produced at high yield from recombinant microorganisms.Type: ApplicationFiled: March 12, 2004Publication date: December 30, 2004Applicant: Kao CorporationInventors: Hiroyuki Araki, Keiji Endo, Hiroshi Hagihara, Kazuaki Igarashi, Yasuhiro Hayashi, Katsuya Ozaki