Patents by Inventor Katsuyuki Shiroguchi

Katsuyuki Shiroguchi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • T-CELL RECEPTOR AND B-CELL RECEPTOR REPERTOIRE ANALYSIS SYSTEM USING ONE-STEP REVERSE TRANSCRIPTION TEMPLATE-SWITCHING PCR
    Publication number: 20190300934
    Abstract: The present invention provides a method for analyzing the variable region repertoire of the T-cell receptors (TCR) or the B-cell receptors (BCR) of a subject, said method including: (1) a step for providing a nucleic acid sample that is amplified from RNA obtained from the subject using one-step reverse transcription template-switching PCR and that includes the nucleic acid sequences of a plurality of types of T-cell receptors (TCR) or B-cell receptors (BCR); (2) a step for determining the nucleic acid sequences included in the nucleic acid sample; and (3) a step for calculating the frequencies of occurrence of individual genes or combinations thereof on the basis of the determined nucleic acid sequences and deriving the TCR or BCR repertoire of the subject.
    Type: Application
    Filed: June 23, 2017
    Publication date: October 3, 2019
    Inventors: Katsuyuki Shiroguchi, Ryuji Suzuki, Takaji Matsutani, Kazutaka Kitaura
  • Single Cell Nucleic Acid Detection and Analysis
    Publication number: 20190211394
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Application
    Filed: March 26, 2019
    Publication date: July 11, 2019
    Inventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia
  • ONE-STEP REVERSE TRANSCRIPTION TEMPLATE-SWITCHING PCR
    Publication number: 20190169679
    Abstract: The present invention provides technology for carrying out one-step reverse transcription template-switching PCR more quickly, more easily, and with high specificity.
    Type: Application
    Filed: June 23, 2017
    Publication date: June 6, 2019
    Inventor: Katsuyuki Shiroguchi
  • Single cell nucleic acid detection and analysis
    Patent number: 10287630
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Grant
    Filed: October 11, 2017
    Date of Patent: May 14, 2019
    Assignee: President and Fellows of Harvard College
    Inventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia
  • Single Cell Nucleic Acid Detection and Analysis
    Publication number: 20180030533
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Application
    Filed: October 11, 2017
    Publication date: February 1, 2018
    Inventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia
  • Single Cell Nucleic Acid Detection and Analysis
    Publication number: 20160130649
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Application
    Filed: January 7, 2016
    Publication date: May 12, 2016
    Inventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia
  • Single cell nucleic acid detection and analysis
    Patent number: 9260753
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Grant
    Filed: March 22, 2012
    Date of Patent: February 16, 2016
    Assignee: President and Fellows of Harvard College
    Inventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z. Jia
  • Single Cell Nucleic Acid Detection and Analysis
    Publication number: 20140155274
    Abstract: Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.
    Type: Application
    Filed: March 22, 2012
    Publication date: June 5, 2014
    Applicant: President and Fellows of Harvard College
    Inventors: Xiaoliang Sunney Xie, Katsuyuki Shiroguchi, Peter A. Sims, Tony Z Jia
  • Loss-loss Mn-Zn ferrite and electronic part made thereof and switching power supply
    Patent number: 7790053
    Abstract: A low-loss Mn—Zn ferrite comprising Fe, Mn and Zn as main components, Co, Ca and Si as first sub-components, and at least one Va-group metal as a second sub-component: Fe and Zn being 53-56% by mol (calculated as Fe2O3) and 1-9% by mol (calculated as ZnO), respectively, and Mn being the balance, based on the total amount (100% by mol) of the main components; Co and Ca being 500-5000 ppm (calculated as Co3O4) and 3000 ppm or less (calculated as CaCO3), respectively, by mass based on the total amount of the main components, a mass ratio of Ca (calculated as CaCO3) to Si (calculated as SiO2) being 2 or more; Ta being 250 ppm or more (calculated as Ta2O5) among the Va-group metals; the ferrite having an average crystal grain size of less than 3.2 ?m and volume resistivity ? of 1 ?·m or more, and a power loss Pcv of 350 kW/m3 or less in a range of 0° C.-120° C. at a frequency of 2 MHz and a magnetic flux density of 25 mT.
    Type: Grant
    Filed: November 21, 2005
    Date of Patent: September 7, 2010
    Assignee: Hitachi Metals, Ltd.
    Inventors: Masao Ishiwaki, Katsuyuki Shiroguchi
  • Loss-Loss Mn-Zn Ferrite and Electronic Part Made Thereof nd Switching Power Supply
    Publication number: 20080007377
    Abstract: A low-loss Mn—Zn ferrite comprising Fe, Mn and Zn as main components, Co, Ca and Si as first sub-components, and at least one Va-group metal as a second sub-component: Fe and Zn being 53-56% by mol (calculated as Fe2O3) and 1-9% by mol (calculated as ZnO), respectively, and Mn being the balance, based on the total amount (100% by mol) of the main components; Co and Ca being 500-5000 ppm (calculated as Co3O4) and 3000 ppm or less (calculated as CaCO3), respectively, by mass based on the total amount of the main components, a mass ratio of Ca (calculated as CaCO3) to Si (calculated as SiO2) being 2 or more; Ta being 250 ppm or more (calculated as Ta2O5) among the Va-group metals; the ferrite having an average crystal grain size of less than 3.2 ?m and volume resistivity ? of 1 ?·m or more, and a power loss Pcv of 350 kW/m3 or less in a range of 0° C.-120° C. at a frequency of 2 MHz and a magnetic flux density of 25 mT.
    Type: Application
    Filed: November 21, 2005
    Publication date: January 10, 2008
    Applicant: HITACHI METALS. LTD.
    Inventors: Masao Ishiwaki, Katsuyuki Shiroguchi