Abstract: Optimizing production of selectively capped, and uncapped, cysteines on antibodies by manipulation of cell growth conditions including the deliberate depletion of cysteine and/or cystine in the cell culture process by way of media components, batch duration, or cell density to achieve efficient production of proteins including antibody-drug-conjugates (ADCs).; conjugating a TNB-capped cysteine-containing protein by reacting it with a reducing agent capable of detaching the TNB-capping moieties from the protein without significantly reducing antibody inter-chain sulfur bonds, and conjugating reduced sulfur bonds on the protein to a payload through a reactive linking moiety.

Abstract: The present invention protocol offers guaranteed Quality of Service (QoS) for a predetermined number of circuits in a highly dynamic, scalable network. The present invention protocol employs a Time Division Multiple Access (TDMA) reservation scheme to transmit deterministic data (e.g., image data) to a destination, while utilizing a Carrier Sense Multiple Access/Collision Avoidance (CSMA/CA) contention scheme to support non-deterministic data traffic. The protocol operates over a link-state based routing protocol that reliably floods routing and resource reservation information to the nodes in the network. Moreover, the present invention capitalizes on certain properties of a radio, such as a RAKE type receiver, to enable simultaneous re-transmissions of resource and route reservation information to constructively interfere and enhance the strength and range of the signal.

Abstract: A wireless communications network including multiple nodes, and a local node having GPS-based time for synchronization of time-of-day (TOD) in the network. The network includes at least the local node and a neighbor node communicating in the network. The local node includes a clock generator for generating a local TOD, and a time management unit, coupled to the clock generator, for adjusting the local TOD. A receiver in the local node receives from the neighbor node a message including a neighbor TOD. A counter in the local node computes and provides an integer value corresponding to a number of update values for synchronization of the neighbor TOD to the GPS-based time. A transmitter in the local node transmits to the neighbor node the integer value provided by the counter, whereby the time management unit adjusts the local TOD to the neighbor TOD and then transmits the adjusted local TOD and the integer value to the neighbor node for synchronization of the neighbor TOD to the GPS-based time.

Abstract: A wireless communications network including multiple nodes, and a local node having GPS-based time for synchronization of time-of-day (TOD) in the network. The network includes at least the local node and a neighbor node communicating in the network. The local node includes a clock generator for generating a local TOD, and a time management unit, coupled to the clock generator, for adjusting the local TOD. A receiver in the local node receives from the neighbor node a message including a neighbor TOD. A counter in the local node computes and provides an integer value corresponding to a number of update values for synchronization of the neighbor TOD to the GPS-based time. A transmitter in the local node transmits to the neighbor node the integer value provided by the counter, whereby the time management unit adjusts the local TOD to the neighbor TOD and then transmits the adjusted local TOD and the integer value to the neighbor node for synchronization of the neighbor TOD to the GPS-based time.