Abstract: (5R)-3-[4-[1-[(2S)-2,3-dihydroxypropanoyl]-3,6-dihydro-2H-pyridin-4-yl]-3,5-difluoro-phenyl]-5-(isoxazol-3-yloxymethyl)oxazolidin-2-one, or a pharmaceutically-acceptable salt, or an in-vivo-hydrolysable ester thereof, for use in the treatment of Mycobacterium tuberculosis.

Abstract: The invention provides an assay for detecting phospho-N-acetylmuramyl-pentapeptide translocase enzyme activity, which comprises the steps of: (1) incubating a reaction mixture comprising, in aqueous medium, N-succinimidyl [2,3-3H] propionate substituted UDP-MurNAc-pentapeptide, N-succinimidyl propionate substituted UDP-MurNAc-pentapeptide (non-radioactive), a source of divalent metal ions, a source of undecaprenyl phosphate, a source of translocase enzyme and a detergent, under conditions suitable for enzyme activity to occur; (2) acidification of the reaction mixture with a suitable buffer comprising a quaternary ammonium salt at pH˜4.2 to stop the enzyme reaction of step (1); and (3) extraction of any undecaprenol-pyrophosphate-[2,3-3H]propionate-N-acetylmuramylpentapeptide product formed and measuring radioactivity using a scintillation counter; and also a kit for use therein.