Abstract: In order to provide a method of testing lymph node metastasis in cervical cancer which can determine the presence of lymph node metastasis in cervical cancer with higher accuracy, the method includes acquiring information about mRNA expression level of at least one gene selected from the group consisting of SERPINB5, CK15, CK4, PI3, and ANXA8 contained in a measurement sample prepared from a lymph node tissue from a cervical cancer patient which may have cervical cancer metastasis; and determining whether cervical cancer has spread to the lymph node tissue based on the information about mRNA expression level acquired.

Abstract: A method of determining a lymph node metastasis in a lung cancer, comprising: acquiring mRNA expression level of stratifin contained in a measurement sample prepared by using a lymph node tissue of a lung cancer patient who may have a lymph node metastasis; and determining that the lymph node tissue is a lymph node metastasis of the lung cancer when the acquired mRNA expression level of stratifin is overexpressed. An apparatus and a computer program product are also disclosed.

Abstract: The present invention provides a means and method for detecting TYR mRNA. As a primer for detecting TYP mRNA, a primer comprising a first sequence at the 5? end side and a second sequence at the 3? end side, wherein the first sequence has 10 to 30 nucleotides in length, and the first sequence is a sequence of a polynucleotide capable of hybridizing with a strand complementary to a first region which is a partial region of SEQ ID NO: 1; and wherein the second sequence has 10 to 30 nucleotides in length, and the second sequence is a sequence of a polynucleotide capable of hybridizing with a second region located in the 3? end side from the first region in SEQ ID NO: 1 is used.

Abstract: Disclosed is a primer for detecting mRNA for CK7. The primer comprises a first sequence on its 5?-terminal side and a second sequence on its 3?-terminal side. The first sequence has a length of 10 to 30 nucleotides, and can hybridize with a strand complementary to a first region which is a region contained in the sequence depicted in SEQ ID NO:1. The second sequence has a length of 10 to 30 nucleotides, and can hybridize with a second region which is located on the 3?-terminal side from the first region in the sequence depicted in SEQ ID NO:1.

Abstract: A method of determining metastasis of a cancer to a lymph node, comprising the steps of: quantitating a polypeptide associated with cytokeratin, in a sample for detection prepared using a lymph node tissue collected from a living body, and determining metastasis of a cancer cell to the lymph node based on the quantitation result of the polypeptide is provided. Thereby, metastasis of a cancer to a lymph node can be determined with high reliance.

Abstract: A method for determining a cancer cell which has good precision and high reliability, including a step of quantitating an mRNA transcribed from a tumor marker gene contained in a biological sample, a step of quantitating a polypeptide translated from the tumor marker gene in the biological sample, and a step of detecting a cancer cell in the biological sample based on the quantitation result of the mRNA and the quantitation result of the polypeptide.

Abstract: A method and apparatus for judging the presence or absence of metastasis of malignant tumor is herein described. The method comprising: measuring an absolute amount of a tumor marker; and judging the presence or absence of metastasis of malignant tumor by comparing the absolute amount with a predetermined threshold value. The apparatus comprising: a measuring assembly for obtaining information related to an absolute amount of a tumor marker; and a computer for obtaining absolute amount of the tumor marker based on the information related to the absolute amount of the tumor marker, and judging the presence or absence of metastasis by comparing the absolute amount of the tumor marker with a predetermined threshold value.

Abstract: A method of detecting a gene as an amplified product includes subjecting a solution containing the gene to a gene amplification reaction at a predetermined reaction temperature in the presence of a surfactant having a higher clouding point than the predetermined reaction temperature and detecting the gene as an amplified product, and a reagent kit for the method of detecting the gene as the amplified product utilizing the gene amplification includes a primer necessary for amplifying the gene, an enzyme for amplifying the gene, and a surfactant having a clouding point higher than the predetermined reaction temperature, wherein the primer is packed separately from the enzyme, and the surfactant is packed with the primer or the enzyme, or separately.

Abstract: An object is to provide a new primer to use in genetic amplification reactions to detect carcinoembryonic antigen (CEA).

Abstract: A method of detecting a gene as an amplified product includes subjecting a solution containing the gene to a gene amplification reaction at a predetermined reaction temperature in the presence of a surfactant having a higher clouding point than the predetermined reaction temperature and detecting the gene as an amplified product, and a reagent kit for the method of detecting the gene as the amplified product utilizing the gene amplification includes a primer necessary for amplifying the gene, an enzyme for amplifying the gene, and a surfactant having a clouding point higher than the predetermined reaction temperature, wherein the primer is packed separately from the enzyme, and the surfactant is packed with the primer or the enzyme, or separately.