Patents by Inventor Kazue Miyake

Kazue Miyake has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 8367328
    Abstract: A DNA synthesis reaction-enhancer comprising at least one kind selected from the group consisting of acidic substances and cationic complexes; a DNA synthesis method in which during a DNA synthesis reaction a reaction is carried out in the presence of the above enhancer by using DNA polymerase; a DNA synthesis reaction composition comprising the above enhancer; a DNA synthesis reaction composition comprising two or more kinds of DNA polymerases each having 3??5? exonuclease activity; a DNA synthesis method in which during a DNA synthesis reaction two or more kinds of DNA polymerases each having 3??5? exonuclease activity are used; a kit for use in in vitro DNA synthesis, comprising two or more kinds of DNA polymerases each having 3??5? exonuclease activity; and a kit for use in in vitro DNA synthesis, wherein the kit comprises the DNA synthesis reaction-enhancer and DNA polymerase.
    Type: Grant
    Filed: October 15, 2008
    Date of Patent: February 5, 2013
    Assignee: Takara Bio Inc.
    Inventors: Kiyozo Asada, Takashi Uemori, Yoshimi Sato, Tomoko Fujita, Kazue Miyake, Osamu Takeda, Hiroyuki Mukai, Ikunoshin Kato
  • Patent number: 7521178
    Abstract: A DNA synthesis reaction-enhancer comprising at least one kind selected from the group consisting of acidic substances and cationic complexes; a DNA synthesis method in which during a DNA synthesis reaction a reaction is carried out in the presence of the above enhancer by using DNA polymerase; a DNA synthesis reaction composition comprising the above enhancer; a DNA synthesis reaction composition comprising two or more kinds of DNA polymerases each having 3??5? exonuclease activity; a DNA synthesis method in which during a DNA synthesis reaction two or more kinds of DNA polymerases each having 3??5? exonuclease activity are used; a kit for use in in vitro DNA synthesis, comprising two or more kinds of DNA polymerases each having 3??5? exonuclease activity; and a kit for use in in vitro DNA synthesis, wherein the kit comprises the DNA synthesis reaction-enhancer and DNA polymerase.
    Type: Grant
    Filed: April 21, 1999
    Date of Patent: April 21, 2009
    Assignee: Takara Bio Inc.
    Inventors: Kiyozo Asada, Takashi Uemori, Yoshimi Sato, Tomoko Fujita, Kazue Miyake, Osamu Takeda, Hiroyuki Mukai, Ikunoshin Kato
  • Publication number: 20090098613
    Abstract: A DNA synthesis reaction-enhancer comprising at least one kind selected from the group consisting of acidic substances and cationic complexes; a DNA synthesis method in which during a DNA synthesis reaction a reaction is carried out in the presence of the above enhancer by using DNA polymerase; a DNA synthesis reaction composition comprising the above enhancer; a DNA synthesis reaction composition comprising two or more kinds of DNA polymerases each having 3??5? exonuclease activity; a DNA synthesis method in which during a DNA synthesis reaction two or more kinds of DNA polymerases each having 3??5? exonuclease activity are used; a kit for use in in vitro DNA synthesis, comprising two or more kinds of DNA polymerases each having 3??5? exonuclease activity; and a kit for use in in vitro DNA synthesis, wherein the kit comprises the DNA synthesis reaction-enhancer and DNA polymerase.
    Type: Application
    Filed: October 15, 2008
    Publication date: April 16, 2009
    Inventors: Kiyozo Asada, Takashi Uemori, Yoshimi Sato, Tomoko Fujita, Kazue Miyake, Osamu Takeda, Hiroyuki Mukai, Ikunoshin Kato
  • Publication number: 20050239100
    Abstract: A convenient and effective method for amplifying a nucleic acid sequence characterized by effecting a DNA synthesis reaction in the presence of chimeric oligonucleotide primers; a method for supplying a large amount of DNA amplification fragments; an effective method for amplifying a nucleic acid sequence by combining the above method with another nucleic acid sequence amplification method; a method for detecting a nucleic acid sequence for detecting or quantitating a microorganism such as a virus, a bacterium, a fungus or a yeast; and a method for detecting a DNA amplification fragment obtained by the above method in situ.
    Type: Application
    Filed: October 27, 2004
    Publication date: October 27, 2005
    Applicant: TAKARA BIO INC.
    Inventors: Hiroyuki Mukai, Hiroaki Sagawa, Takashi Uemori, Junko Yamamoto, Jun Tomono, Eiji Kobayashi, Tatsuji Enoki, Osamu Takeda, Kazue Miyake, Yoshimi Sato, Mariko Moriyama, Haruhisa Sawaragi, Michio Hagiya, Kiyozo Asada, Ikunoshin Kato
  • Patent number: 6951722
    Abstract: A convenient and effective method for amplifying a nucleic acid sequence characterized by effecting a DNA synthesis reaction in the presence of chimeric oligonucleotide primers; a method for supplying a large amount of DNA amplification fragments; an effective method for amplifying a nucleic acid sequence by combining the above method with another nucleic acid sequence amplification method; a method for detecting a nucleic acid sequence for detecting or quantitating a microorganism such as a virus, a bacterium, a fungus or a yeast; and a method for detecting a DNA amplification fragment obtained by the above method in situ.
    Type: Grant
    Filed: August 23, 2001
    Date of Patent: October 4, 2005
    Assignee: Takara Bio Inc.
    Inventors: Hiroyuki Mukai, Hiroaki Sagawa, Takashi Uemori, Junko Yamamoto, Jun Tomono, Eiji Kobayashi, Tatsuji Enoki, Osamu Takeda, Kazue Miyake, Yoshimi Sato, Mariko Moriyama, Haruhisa Sawaragi, Michio Hagiya, Kiyozo Asada, Ikunoshin Kato
  • Patent number: 6949634
    Abstract: A method for synthesizing cDNA characterized by performing a reverse transcription reaction in the presence of an enzyme having a reverse transcriptional activity and another enzyme different from the former one which as a 3?-5? exonuclease activity.
    Type: Grant
    Filed: July 24, 2002
    Date of Patent: September 27, 2005
    Assignee: Takara Shuzo Co., Ltd.
    Inventors: Junko Yamamoto, Kazue Miyake, Hiroyuki Mukai, Fumitsugu Hino, Ikunoshin Kato
  • Publication number: 20050153326
    Abstract: A method for synthesizing cDNA characterized by performing a reverse transcription reaction in the presence of an enzyme having a reverse transcriptional activity and another enzyme different from the former one which as a 3?-5? exonuclease activity.
    Type: Application
    Filed: November 23, 2004
    Publication date: July 14, 2005
    Applicant: TAKARA SHUZO CO., LTD.
    Inventors: Junko Yamamoto, Kazue Miyake, Hiroyuki Mukai, Fumitsugu Hino, Ikunoshin Kato
  • Publication number: 20050123950
    Abstract: A convenient and effective method for amplifying a nucleic acid sequence characterized by effecting a DNA synthesis reaction in the presence of chimeric oligonucleotide primers; a method for supplying a large amount of DNA amplification fragments; an effective method for amplifying a nucleic acid sequence by combining the above method with another nucleic acid sequence amplification method; a method for detecting a nucleic acid sequence for detecting or quantitating a microorganism such as a virus, a bacterium, a fungus or a yeast; and a method for detecting a DNA amplification fragment obtained by the above method in situ.
    Type: Application
    Filed: August 31, 2004
    Publication date: June 9, 2005
    Applicant: TAKARA BIO NIC.
    Inventors: Hiroyuki Mukai, Hiroaki Sagawa, Takashi Uemori, Junko Yamamoto, Jun Tomono, Eiji Kobayashi, Tatsuji Enoki, Osamu Takeda, Kazue Miyake, Yoshimi Sato, Mariko Moriyama, Haruhisa Sawaragi, Michio Hagiya, Kiyozo Asada, Ikunoshin Kato
  • Patent number: 6673578
    Abstract: A DNA synthesis method with a shortened time period required for DNA synthesis by polymerase chain reaction (PCR), characterized in that a DNA polymerase is used in an amount effective for providing more than 10 ng of amplified DNA fragments of about 2 kb per 50 &mgr;l of a reaction mixture, when PCR is carried out under the following conditions (A) and (B): (A) reaction mixture: 50 &mgr;l volume of a reaction mixture comprising DNA polymerase, 1 ng of genomic DNA from Escherichia coli, and 10 pmol each of primers Eco-1 and Eco-2 (nucleotide sequences of the primers Eco-1 and Eco-2 being shown in SEQ ID NOs: 10 and 11 of Sequence Listing, respectively); and having a composition suitable for the DNA polymerase; and (B) reaction conditions: 35 cycles of PCR, wherein one cycle consists of 99° C., 1 second-66° C., 7 seconds; a kit for DNA synthesis usable for the DNA synthesis method; and an article of manufacture of a PCR agent.
    Type: Grant
    Filed: May 22, 2001
    Date of Patent: January 6, 2004
    Assignee: Takara Shuzo Co., Ltd.
    Inventors: Takashi Uemori, Yoshimi Sato, Mariko Okawa, Tomoko Fujita, Kazue Miyake, Osamu Takeda, Hiroaki Sagawa, Michio Hagiya, Hiroyuki Mukai, Kiyozo Asada, Ikunoshin Kato
  • Publication number: 20030186312
    Abstract: A DNA synthesis method with a shortened time period required for DNA synthesis by polymerase chain reaction (PCR), characterized in that a DNA polymerase is used in an amount effective for providing more than 10 ng of amplified DNA fragments of about 2 kb per 50 &mgr;l of a reaction mixture, when PCR is carried out under the following conditions (A) and (B): (A) reaction mixture: 50 &mgr;l volume of a reaction mixture comprising DNA polymerase, 1 ng of genomic DNA from Escherichia coli, and 10 pmol each of primers Eco-1 and Eco-2 (nucleotide sequences of the primers Eco-1 and Eco-2 being shown in SEQ ID NOs: 10 and 11 of Sequence Listing, respectively); and having a composition suitable for the DNA polymerase; and (B) reaction conditions: 35 cycles of PCR, wherein one cycle consists of 99° C., 1 second-66° C., 7 seconds; a kit for DNA synthesis usable for the DNA synthesis method; and an article of manufacture of a PCR agent.
    Type: Application
    Filed: May 12, 2003
    Publication date: October 2, 2003
    Applicant: Takara Shuzo Co., Ltd.
    Inventors: Takashi Uemori, Yoshimi Sato, Mariko Okawa, Tomoko Fujita, Kazue Miyake, Osamu Takeda, Hiroaki Sagawa, Michio Hagiya, Hiroyuki Mukai, Kiyozo Asada, Ikunoshin Kato
  • Publication number: 20030077762
    Abstract: A method for synthesizing cDNA characterized by performing a reverse transcription reaction in the presence of an enzyme having a reverse transcriptional activity and another enzyme different from the former one which as a 3′-5′ exonuclease activity.
    Type: Application
    Filed: July 24, 2002
    Publication date: April 24, 2003
    Applicant: TAKARA SHUZO CO., LTD.
    Inventors: Junko Yamamoto, Kazue Miyake, Hiroyuki Mukai, Fumitsugu Hino, Ikunoshin Kato
  • Publication number: 20030073081
    Abstract: A convenient and effective method for amplifying a nucleic acid sequence characterized by effecting a DNA synthesis reaction in the presence of chimeric oligonucleotide primers; a method for supplying a large amount of DNA amplification fragments; an effective method for amplifying a nucleic acid sequence by combining the above method with another nucleic acid sequence amplification method; a method for detecting a nucleic acid sequence for detecting or quantitating a microorganism such as a virus, a bacterium, a fungus or a yeast; and a method for detecting a DNA amplification fragment obtained by the above method in situ.
    Type: Application
    Filed: August 23, 2001
    Publication date: April 17, 2003
    Applicant: Takara Shuzo Co., Ltd
    Inventors: Hiroyuki Mukai, Hiroaki Sagawa, Takashi Uemori, Junko Yamamoto, Jun Tomono, Eiji Kobayashi, Tatsuji Enoki, Osamu Takeda, Kazue Miyake, Yoshimi Sato, Mariko Moriyama, Haruhisa Sawaragi, Michio Hagiya, Kiyozo Asada, Ikunoshin Kato
  • Patent number: 6485917
    Abstract: A method for synthesizing cDNA characterized by performing a reverse transcription reaction in the presence of an enzyme having a reverse transcriptional activity and another enzyme different from the former one which as a 3′-5′ exonuclease activity.
    Type: Grant
    Filed: May 23, 2001
    Date of Patent: November 26, 2002
    Assignee: Takara Shuzo Co., Ltd.
    Inventors: Junko Yamamoto, Kazue Miyake, Hiroyuki Mukai, Fumitsugu Hino, Ikunoshin Kato
  • Publication number: 20020106675
    Abstract: The present invention relates to a thermostable DNA polymerase-associated factor capable of enhancing DNA synthesizing-activity of a DNA polymerase; a thermostable DNA polymerase-associated factor possessing an activity of binding to a DNA polymerase and a method for producing the same; a gene encoding the DNA polymerase-associated factor; a method of DNA synthesis by using a DNA polymerase in the presence of the DNA polymerase-associated factor; and a kit comprising the DNA polymerase-associated factor. According to the present invention, there can be provided in vitro DNA synthesis and a DNA amplification system which are more excellent than conventional techniques by utilizing the DNA polymerase-associated factor of the present invention.
    Type: Application
    Filed: October 5, 2001
    Publication date: August 8, 2002
    Applicant: Takara Shuzo Co., Ltd.
    Inventors: Takashi Uemori, Yoshimi Sato, Tomoko Fujita, Kazue Miyake, Hiroyuki Mukai, Kiyozo Asada, Ikunoshin Kato
  • Patent number: 6333158
    Abstract: The present invention relates to a thermostable DNA polymerase-associated factor capable of enhancing DNA synthesizing-activity of a DNA polymerase; a thermostable DNA polymerase-associated factor possessing an activity of binding to a DNA polymerase and a method for producing the same; a gene encoding the DNA polymerase-associated factor; a method of DNA synthesis by using a DNA polymerase in the presence of the DNA polymerase-associated factor; and a kit comprising the DNA polymerase-associated factor. According to the present invention, there can be provided in vitro DNA synthesis and a DNA amplification system which are more excellent than conventional techniques by utilizing the DNA polymerase-associated factor of the present invention.
    Type: Grant
    Filed: November 15, 2000
    Date of Patent: December 25, 2001
    Assignee: Takara Shuzo Co., Ltd.
    Inventors: Takashi Uemori, Yoshimi Sato, Tomoko Fujita, Kazue Miyake, Hiroyuki Mukai, Kiyozo Asada, Ikunoshin Kato
  • Patent number: 6218150
    Abstract: The present invention relates to a thermostable DNA polymerase-associated factor capable of enhancing DNA synthesizing-activity of a DNA polymerase; a thermostable DNA polymerase-associated factor possessing an activity of binding to a DNA polymerase and a method for producing the same; a gene encoding the DNA polymerase-associated factor; a method of DNA synthesis by using a DNA polymerase in the presence of the DNA polymerase-associated factor; and a kit comprising the DNA polymerase-associated factor. According to the present invention, there can be provided in vitro DNA synthesis and a DNA amplification system which are more excellent than conventional techniques by utilizing the DNA polymerase-associated factor of the present invention.
    Type: Grant
    Filed: December 23, 1999
    Date of Patent: April 17, 2001
    Assignee: Takara Shuzo Co., Ltd.
    Inventors: Takashi Uemori, Yoshimi Sato, Tomoko Fujita, Kazue Miyake, Hiroyuki Mukai, Kiyozo Asada, Ikunoshin Kato