Patents by Inventor Kazuhiro Fukae
Kazuhiro Fukae has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20120047586Abstract: Disclosed is a polypeptide having an enhanced effector function. Specifically disclosed are: a polypeptide having a modified Fc region; a nucleic acid encoding the polypeptide; a vector carrying the nucleic acid; a host cell or a host organism harboring the vector; a pharmaceutical composition comprising the polypeptide; a method for producing the polypeptide; a method for enhancing the effector function of an antibody; and a method for producing a cell capable of producing an antibody having a high effector function.Type: ApplicationFiled: October 23, 2008Publication date: February 23, 2012Applicant: Otsuka Chemical Co., LtdInventors: Takashi Tsuji, Yasuhiro Kajihara, Katsunari Tezuka, Yuri Nambu, Kazuhiro Fukae, Hiroaki Asai
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Publication number: 20110313136Abstract: A full-length IgG-Fc fragment having a substantially homogeneous sugar chain added thereto, and a process for producing the full-length IgG-Fc fragment. Specifically, an IgG-Fc fragment has a sugar chain added thereto, in which the sugar chain is added to the same position as that in a naturally occurring IgG-Fc fragment, any one amino acid residue selected from 1st to 30th amino acid residues from the sugar chain-added amino acid residue on the N-terminal side of the sugar chain-added amino acid residue is substituted by a Cys residue and at least one Met reside is substituted by an amino acid reside other than a Met residue.Type: ApplicationFiled: February 9, 2010Publication date: December 22, 2011Applicant: OTSUKA CHEMICAL CO., LTD.Inventors: Yasuhiro Kajihara, Kazuhiro Fukae
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Patent number: 8058394Abstract: The present invention provides a process for producing a peptide thioester compound. The process involves: (A) forming a peptide by a solid-phase synthesis method using a resin modified with a linker represented by the formula (1) as a solid phase: wherein R1 represents C1-4 alkyl group, R2 represents hydrogen atom or C1-4 alkoxy group, and n represents an integer of 1 to 4; (B) cleaving a bond between the solid phase and the peptide with at least one acid selected from dilute hydrochloric acid, dilute sulfuric acid, formic acid, and acetic acid to produce a peptide having a carboxyl group at the C-terminus; and (C) reacting a thiol compound with the peptide at ?100 to 0° C. in the presence of a condensing agent in a solvent.Type: GrantFiled: March 28, 2007Date of Patent: November 15, 2011Assignee: Otsuka Chemical Co., Ltd.Inventors: Yasuhiro Kajihara, Naoki Yamamoto, Yuri Nambu, Kazuhiro Fukae, Hiroaki Asai
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Publication number: 20110262945Abstract: A method for producing a glycoprotein, which is uniform in terms of functions derived from a sugar chain (e.g., a blood half-life) and physiological activities, i.e., a glycoprotein, which is uniform in terms of the amino acid sequence, the sugar chain structure and the higher-order structure; specifically, a method for producing a glycoprotein, which is uniform in terms of the amino acid sequence, the sugar chain structure, and the higher-order structure, includes the following steps (a) to (c): (a) folding a glycoprotein, which is uniform in terms of the amino acid sequence and the sugar chain structure; (b) fractionating the folded glycoprotein by column chromatography; and (c) collecting a fraction having a specified activity.Type: ApplicationFiled: August 18, 2009Publication date: October 27, 2011Applicant: OTSUKA CHEMICAL CO., LTD.Inventors: Yasuhiro Kajihara, Kazuhiro Fukae
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Publication number: 20110245166Abstract: The present invention relates to an oligosaccharide chain added GLP-1 peptide that has higher stability in blood than that of GLP-1 and, preferably, exhibits higher activity of controlling blood-sugar levels than that of GLP-1. The present invention relates to an oligosaccharide chain added GLP-1 peptide having GLP-1 activity, wherein at least one amino acid is substituted with an oligosaccharide chain added amino acid, in: (a) GLP-1; (b) a peptide having the amino acid sequence of GLP-1 with deletion, substitution or addition of one or several amino acids; or (c) a GLP-1 analog.Type: ApplicationFiled: June 2, 2011Publication date: October 6, 2011Applicant: OTSUKA CHEMICAL HOLDINGS CO., LTDInventors: Yasuhiro KAJIHARA, Takashi TSUJI, Izumi SAKAMOTO, Yuri NAMBU, Kazuhiro FUKAE, Katsunari TEZUKA, Hiroaki ASAI
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Publication number: 20110195897Abstract: Oligosaccharide chain added GLP-1 peptides are more stable in blood and more active in controlling blood-sugar levels than GLP-1 peptides without added oligosaccharides. Oligosaccharide chain added GLP-1 peptides having GLP-1 activity include at least one or at least two amino acids each substituted with an oligosaccharide chain added amino acid in GLP-1; a peptide having the amino acid sequence of GLP-1 with deletion, substitution or addition of one or several amino acids; or a GLP-1 analog. Oligosaccharide chain added GLP-1 peptides with at least one amino acid substituted with an oligosaccharide chain added amino acid include an oligosaccharide chain with oligo hyaluronic acid. Oligosaccharide chain added amino acids include oligosaccharide chains attached to amino acids via linkers.Type: ApplicationFiled: June 15, 2009Publication date: August 11, 2011Applicant: OTSUKA CHEMICAL CO., LTD.Inventors: Yasuhiro Kajihara, Takashi Tsuji, Izumi Sakamoto, Yuri Nambu, Naohiro Hayashi, Kazuyuki Ishii, Kazuhiro Fukae, Katsunari Tezuka, Hiroaki Asai
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Patent number: 7985731Abstract: The present invention relates to an oligosaccharide chain added GLP-1 peptide that has higher stability in blood than that of GLP-1 and, preferably, exhibits higher activity of controlling blood-sugar levels than that of GLP-1. The present invention relates to an oligosaccharide chain added GLP-1 peptide having GLP-1 activity, wherein at least one amino acid is substituted with an oligosaccharide chain added amino acid, in: (a) GLP-1; (b) a peptide having the amino acid sequence of GLP-1 with deletion, substitution or addition of one or several amino acids; or (c) a GLP-1 analog.Type: GrantFiled: June 17, 2008Date of Patent: July 26, 2011Assignee: Otsuka Chemical Holdings Co., Ltd.Inventors: Yasuhiro Kajihara, Takashi Tsuji, Izumi Sakamoto, Yuri Nambu, Kazuhiro Fukae, Katsunari Tezuka, Hiroaki Asai
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Publication number: 20110172392Abstract: The present invention provides a method for producing a peptide, characterized in that it comprises converting an —SH group of a peptide comprising an amino acid residue having the —SH group to an —OH group, wherein said method comprises the following steps (a) to (c): (a) allowing an —SH group in a peptide to react with a methylating agent to convert the —SH group to an —SMe group; (b) allowing the —SMe group obtained in the step (a) to react with a cyanizing agent to produce a reaction intermediate; and (c) converting the reaction intermediate obtained in the step (b) to a peptide comprising an amino acid residue having an —OH group under more basic conditions than the conditions in the step (b).Type: ApplicationFiled: July 30, 2008Publication date: July 14, 2011Applicant: OTSUKA CHEMICAL CO., LTD.Inventors: Yasuhiro Kajihara, Izumi Sakamoto, Yuri Nambu, Kazuhiro Fukae, Hiroaki Asai
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Patent number: 7955819Abstract: A process for preparing asparagine-linked oligosaccharide derivatives including the steps of: (a) treating a delipidated egg yolk with a protease to obtain a mixture of peptide-linked oligosaccharides, (b) treating the mixture of peptide-linked oligosaccharides with a peptidase to obtain a mixture of asparagine-linked oligosaccharides, (c) introducing a lipophilic protective group into the asparagine-linked oligosaccharides in the mixture to obtain a mixture of asparagine-linked oligosaccharide derivatives, and (d) subjecting the mixture of asparagine-linked oligosaccharide derivatives to chromatography to separate the mixture into individual asparagine-linked oligosaccharide derivatives.Type: GrantFiled: February 3, 2004Date of Patent: June 7, 2011Assignee: Otsuka Chemical Holdings Co., Ltd.Inventor: Kazuhiro Fukae
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Patent number: 7851618Abstract: An aminated complex-type oligosaccharide derivative of the formula (1) wherein R1 is H—(CO)—CH2X, —NH—(CO)—(CH2)b—CH2X, isothiocyanate group, —NH—(CO)a—(CH2)b—CO2H or —NH—(CO)a—(CH2)b—CHO, X being a halogen atom, a being 0 or 1, b being an integer of 1 to 4, R2 and R3 are a hydrogen atom or a group of the formulae (2) to (5) and may be the same or different, except for the case where both R2 and R3 are hydrogen or the formula (5), and the case where one of R2 and R3 is a hydrogen atom, with the formula (5) serving as the other thereof.Type: GrantFiled: July 27, 2004Date of Patent: December 14, 2010Assignee: Otsuka Chemical Co., Ltd.Inventors: Yasuhiro Kajihara, Kazuhiro Fukae
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Publication number: 20100306864Abstract: Disclosed is a polypeptide having an enhanced effector function. Specifically disclosed are: a polypeptide having a modified Fc region; a nucleic acid encoding the polypeptide; a vector carrying the nucleic acid; a host cell or a host organism harboring the vector; a pharmaceutical composition comprising the polypeptide; a method for producing the polypeptide; a method for enhancing the effector function of an antibody; and a method for producing a cell capable of producing an antibody having a high effector function.Type: ApplicationFiled: October 23, 2008Publication date: December 2, 2010Applicant: Otsuka Chemical Co., LtdInventors: Takashi Tsuji, Yasuhiro Kajihara, Katsunari Tezuka, Yuri Nambu, Kazuhiro Fukae, Hiroaki Asai
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Publication number: 20100080794Abstract: Mutant polypeptides of the present invention contain an Fc region in which a cysteine residue is substituted for the second amino acid from the glycosylation site to the N terminal side in the Fc region. The Fc region is preferably a human IgG Fc region. The mutant polypeptides of the present invention may also contain an N-linked sugar chain at the glycosylation site in Fc region. Furthermore, a polypeptide domain other than the Fc region of the mutant polypeptides of the present invention may be a polypeptide molecule that recognizes a human cell surface molecule.Type: ApplicationFiled: April 12, 2007Publication date: April 1, 2010Inventors: Takashi Tsuji, Yasuhiro Kajihara, Yuri Nambu, Kazuhiro Fukae, Hiroaki Asai, Akihiro Murakami
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Patent number: 7687478Abstract: An asparagine-linked disialoundecaoligosaccharide-fatty acid amide, a drug containing the same, and a drug containing an asparagine-linked disialoundecaoligosaccharide.Type: GrantFiled: June 29, 2004Date of Patent: March 30, 2010Assignee: Otsuka Chemical Co., Ltd.Inventors: Yasuhiro Kajihara, Hiroaki Maeda, Kazuhiro Fukae
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Publication number: 20090137780Abstract: The present invention provides a process for producing a peptide thioester compound, characterized by comprising: (A) forming a peptide by a solid-phase synthesis method using a resin modified with a linker represented by the formula (1) as a solid phase; (B) cleaving a bond between the solid phase and the peptide with at least one acid selected from dilute hydrochloric acid, dilute sulfuric acid, formic acid, and acetic acid to produce a peptide having a carboxyl group at the C-terminus; and (C) reacting a thiol compound with the peptide at ?100 to 0° C. in the presence of a condensing agent in a solvent: (1) wherein R1 represents C1-4 alkyl group, R2 represents hydrogen atom or C1-4 alkoxy group, and n represents an integer of 1 to 4.Type: ApplicationFiled: March 28, 2007Publication date: May 28, 2009Applicant: OTSUKA CHEMICAL CO., LTD.Inventors: Yasuhiro Kajihara, Naoki Yamamoto, Yuri Nambu, Kazuhiro Fukae, Hiroaki Asai
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Publication number: 20090111739Abstract: The present invention relates to an oligosaccharide chain added GLP-1 peptide that has higher stability in blood than that of GLP-1 and, preferably, exhibits higher activity of controlling blood-sugar levels than that of GLP-1. The present invention relates to an oligosaccharide chain added GLP-1 peptide having GLP-1 activity, wherein at least one amino acid is substituted with an oligosaccharide chain added amino acid, in: (a) GLP-1; (b) a peptide having the amino acid sequence of GLP-1 with deletion, substitution or addition of one or several amino acids; or (c) a GLP-1 analog.Type: ApplicationFiled: June 17, 2008Publication date: April 30, 2009Applicant: OTSUKA CHEMICAL CO., LTD.Inventors: Yasuhiro Kajihara, Takashi Tsuji, Izumi Sakamoto, Yuri Nambu, Kazuhiro Fukae, Katsunari Tezuka, Hiroaki Asai
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Publication number: 20080214798Abstract: An asparagine-linked ?2,3-oligosaccharide having undeca- to hepta-saccharides, an asparagine-linked ?2,6-oligosaccharide having undeca- to hepta-saccharides and containing fluorine and an asparagine-linked oligosaccharide derivative containing at least one fucose in N-acetylglucosamine on the nonreducing terminal side of an asparagine-linked oligosaccharide wherein the asparagine has amino group protected with a lipophilic protective group, and a process for producing these compounds.Type: ApplicationFiled: October 25, 2007Publication date: September 4, 2008Applicant: OTSUKA CHEMCIAL CO., LTD.Inventors: Yasuhiro Kajihara, Kazuaki Kakehi, Kazuhiro Fukae
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Patent number: 7304148Abstract: An asparagine-linked ?2,3-oligosaccharide having undeca- to hepta-saccharides, an asparagine-linked ?2,6-oligosaccharide having undeca- to hepta-saccharides and containing fluorine and an asparagine-linked oligosaccharide derivative containing at least one fucose in N-acetylglucosamine on the nonreducing terminal side of an asparagine-linked oligosaccharide wherein the asparagine has amino group protected with a lipophilic protective group, and a process for producing these compounds.Type: GrantFiled: December 24, 2003Date of Patent: December 4, 2007Assignee: Otsuka Chemical Co., Ltd.Inventors: Yasuhiro Kajihara, Kazuaki Kakehi, Kazuhiro Fukae
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Patent number: 7273934Abstract: A 3-branched asparagine-linked oligosaccharide derivative of the formula (1) wherein the nitrogen of amino group of asparagine is modified with a lipophilic protective group, biotin group or FITC group; a 3-branched asparagine-linked oligosaccharide derivative which contains at least one fucose in N-acetylglucosamine on the nonreducing terminal side of the asparagine-linked oligosaccharide of the derivative; asparagine-linked oligosaccharides and oligosaccharides thereof wherein Q is a lipophilic protective group, biotin group or FITC group.Type: GrantFiled: December 26, 2003Date of Patent: September 25, 2007Assignees: Otsuka Chemical Co., Ltd.Inventors: Yasuhiro Kajihara, Kazuaki Kakehi, Kazuhiro Fukae
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Publication number: 20070105813Abstract: An asparagine-linked disialoundecaoligosaccharide-fatty acid amide, a drug containing the same, and a drug containing an asparagine-linked disialoundecaoligosaccharide.Type: ApplicationFiled: June 29, 2004Publication date: May 10, 2007Applicant: OTSUKA CHEMICAL CO., LTD.Inventors: Yasuhiro Kajihara, Hiroaki Maeda, Kazuhiro Fukae
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Publication number: 20070060543Abstract: An aminated complex-type oligosaccharide derivative, for example, of the formula (1) wherein R1 is H—(CO)—CH2X, —NH—(CO)—(CH2)b—CH2X, isothiocyanate group, —NH—(CO)a—(CH2)b—CO2H or —NH—(CO)a—(CH2)b—CHO, X being a halogen atom, a being 0 or 1, b being an integer of 1 to 4, R2 and R3 are a hydrogen atom or a group of the formulae (2) to (5) and may be the same or different, except for the case where both R2 and R3 are hydrogen or the formula (5), and the case where one of R2 and R3 is a hydrogen atom, with the formula (5) serving as the other thereof.Type: ApplicationFiled: July 27, 2004Publication date: March 15, 2007Inventors: Yasuhiro Kajihara, Kazuhiro Fukae