Abstract: Provided is an analysis method in which increase in a background can be prevented in a simple manner without cost. The analysis method of the present invention is carried out using a sample analysis tool 10 including a development member 11 in which a developing solution supply portion 12, a sample supply portion 13, and a detection portion 14 in which a substance 17 that specifically binds to an analyte 16 in a sample is immobilized are provided in this order from upstream to downstream along the flow of a developing solution. A sample solution is supplied to the sample supply portion 13, and a developing solution is supplied to the developing solution supply portion 12 simultaneously with the supply of the sample solution or prior to the supply of the sample solution. By the development of the developing solution in the development member 11 in the presence of a labeled specifically binding substance 15, the sample solution is introduced to the detection portion 14.

Abstract: Provide is a sample analysis tool whose reactivity and reproducibility in analysis can be prevented from decreasing. The sample analysis tool 10 of the present invention includes a development member 11 and a plastic base 16, and at least part of the development member 11 is in contact with the plastic base 16. The sample analysis tool 10 further includes a hydrophilic component layer 15, and the hydrophilic component layer 15 is formed on part or the whole of at least one of a surface of the plastic base 16 and a surface of the development member 11. It is particularly preferable that the hydrophilic component layer 15 contains sucrose or N-methyl glucosamine. In the sample analysis tool 10 of the present invention, since the hydrophilic component layer 15 is formed on part or the whole of at least one of the surface of the plastic base 16 and the surface of the development member 11, it is possible to prevent the adhesion of a hydrophobic component(s) derived from the plastic base 16.

Abstract: A reducing power analysis method for minimizing peak wavelength shift in a sample, comprising a reduction step of reducing a dye reagent containing a ferric compound and a cyanide at pH conditions of 2.4 or lower in the presence of a sample; and an optical measurement step of optically measuring a peak wavelength of a reduced form of the dye reagent obtained in the reduction step.

Abstract: A specimen supplying tool in which structure is simple, and a liquid specimen and air bubbles do not remain within a through-bore for introduction of a specimen, is provided. The specimen supplying tool includes a substrate 11 and a through-bore 13 provided in the substrate 11. One opening of the through-bore 13 is a specimen inflow port, and the other opening of the through-bore 13 is a specimen outflow port. The specimen supplying tool further includes a projection portion 14 projecting from a part p of a marginal part of the specimen outflow port. A liquid specimen is introduced from the specimen inflow port to the through-bore 13. When the introduced liquid specimen is discharged from the specimen outflow port, and supplied to a specimen analyzing instrument, the liquid specimen is drawn to the projection portion 14, led by the projection portion 14, and supplied to the specimen analyzing instrument.

Abstract: A reducing power analysis method for minimizing peak wavelength shift in a sample, comprising a reduction step of reducing a dye reagent containing a ferric compound and a cyanide at pH conditions of 2.4 or lower in the presence of a sample; and an optical measurement step of optically measuring a peak wavelength of a reduced form of the dye reagent obtained in the reduction step.

Abstract: The present invention provides a method of pretreating a specimen, which allows measurement according to an immunoassay to be carried out on a specimen from nasal secretion while preventing non-specific reactions. According to this method, the specimen from nasal secretion is treated with a protease beforehand and then an immunoassay is performed. As the protease, it is preferable to use semi-alkaline protease (EC 3.4.21.63). Furthermore, it is preferable that a substance to be pretreated by the pretreatment method according to the present invention is an influenza virus contained in the specimen from nasal secretion. The immunoassay preferably is an immunoagglutination assay. Examples of the immunoagglutination assay include a turbidimetric immunoassay, a latex turbidimetric immunoassay, and a latex agglutination assay that is performed on a slide glass.

Abstract: An optical measurement apparatus is provided for conducting a test by efficiently reading color development of a reagent after reaction by optical measurement. To the above end, an optical measurement apparatus is provided which is to be used with at least one test instrument mounted to the apparatus. The test instrument includes a carrier provided with at least one reagent retaining portion which retains a reagent, and a sample is applied to the carrier. The optical measurement apparatus includes a reader for reading color development of the reagent retaining portion, and a controller for performing driving control of the reader and determination. The controller performs the determination by utilizing data obtained by a reading operation P to read the color development of the reagent performed after the lapse of a reaction completion period Tr1-Tr6 from the mounting of the test instrument, and the reaction completion period depends on the reagent.

Abstract: Provide is a sample analysis tool whose reactivity and reproducibility in analysis can be prevented from decreasing. The sample analysis tool 10 of the present invention includes a development member 11 and a plastic base 16, and at least part of the development member 11 is in contact with the plastic base 16. The sample analysis tool 10 further includes a hydrophilic component layer 15, and the hydrophilic component layer 15 is formed on part or the whole of at least one of a surface of the plastic base 16 and a surface of the development member 11. It is particularly preferable that the hydrophilic component layer 15 contains sucrose or N-methyl glucosamine. In the sample analysis tool 10 of the present invention, since the hydrophilic component layer 15 is formed on part or the whole of at least one of the surface of the plastic base 16 and the surface of the development member 11, it is possible to prevent the adhesion of a hydrophobic component(s) derived from the plastic base 16.

Abstract: Provided is an analysis method in which increase in a background can be prevented in a simple manner without cost. The analysis method of the present invention is carried out using a sample analysis tool 10 including a development member 11 in which a developing solution supply portion 12, a sample supply portion 13, and a detection portion 14 in which a substance 17 that specifically binds to an analyte 16 in a sample is immobilized are provided in this order from upstream to downstream along the flow of a developing solution. A sample solution is supplied to the sample supply portion 13, and a developing solution is supplied to the developing solution supply portion 12 simultaneously with the supply of the sample solution or prior to the supply of the sample solution. By the development of the developing solution in the development member 11 in the presence of a labeled specifically binding substance 15, the sample solution is introduced to the detection portion 14.

Abstract: The present invention provides a method of pretreating a specimen, which allows measurement according to an immunoassay to be carried out on a specimen from nasal secretion while preventing non-specific reactions. According to this method, the specimen from nasal secretion is treated with a protease beforehand and then an immunoassay is performed. As the protease, it is preferable to use semi-alkaline protease (EC 3.4.21.63). Furthermore, it is preferable that a substance to be pretreated by the pretreatment method according to the present invention is an influenza virus contained in the specimen from nasal secretion. The immunoassay preferably is an immunoagglutination assay. Examples of the immunoagglutination assay include a turbidimetric immunoassay, a latex turbidimetric immunoassay, and a latex agglutination assay that is performed on a slide glass.

Abstract: An optical measurement apparatus is provided for conducting a test by efficiently reading color development of a reagent after reaction by optical measurement. To the above end, an optical measurement apparatus is provided which is to be used with at least one test instrument mounted to the apparatus. The test instrument includes a carrier provided with at least one reagent retaining portion which retains a reagent, and a sample is applied to the carrier. The optical measurement apparatus includes a reader for reading color development of the reagent retaining portion, and a controller for performing driving control of the reader and determination. The controller performs the determination by utilizing data obtained by a reading operation P to read the color development of the reagent performed after the lapse of a reaction completion period Tr1-Tr6 from the mounting of the test instrument, and the reaction completion period depends on the reagent.

Abstract: A specimen supplying tool in which structure is simple, and a liquid specimen and air bubbles do not remain within a through-bore for introduction of a specimen, is provided. The specimen supplying tool includes a substrate 11 and a through-bore 13 provided in the substrate 11. One opening of the through-bore 13 is a specimen inflow port, and the other opening of the through-bore 13 is a specimen outflow port. The specimen supplying tool further includes a projection portion 14 projecting from a part p of a marginal part of the specimen outflow port. A liquid specimen is introduced from the specimen inflow port to the through-bore 13. When the introduced liquid specimen is discharged from the specimen outflow port, and supplied to a specimen analyzing instrument, the liquid specimen is drawn to the projection portion 14, led by the projection portion 14, and supplied to the specimen analyzing instrument.

Abstract: In an immunoassay method using an antibody sensitization particle, the occurrence of false positives is prevented. The immunoassay method of the present invention is an immunoassay method for detecting or quantifying an analyte in a specimen by mixing an antibody sensitization particle obtained by sensitizing a particle with an antibody that reacts with the analyte in the specimen with the specimen, and measuring agglutination of the antibody sensitization particle by an antigen-antibody reaction, and the method includes pre-treating the specimen by using a cation exchange material in advance of the mixing step. As the cation exchange material, for example, a cation exchange material having a sulfonic acid group or a carboxyl group as a functional group can be used, and a form thereof is not limited particularly, and may be, for example, a cation exchange filter, a cation exchange column or the like.

Abstract: The present invention provides a method of pretreating a specimen, which allows measurement according to an immunoassay to be carried out on a specimen from nasal secretion while preventing non-specific reactions. According to this method, the specimen from nasal secretion is treated with a protease beforehand and then an immunoassay is performed. As the protease, it is preferable to use semi-alkaline protease (EC 3.4.21.63). Furthermore, it is preferable that a substance to be pretreated by the pretreatment method according to the present invention is an influenza virus contained in the specimen from nasal secretion. The immunoassay preferably is an immunoagglutination assay. Examples of the immunoagglutination assay include a turbidimetric immunoassay, a latex turbidimetric immunoassay, and a latex agglutination assay that is performed on a slide glass.