Patents by Inventor Kazuhiro Ohsuye
Kazuhiro Ohsuye has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7807421Abstract: The present invention provides a transformed cell which is transformed by at least one gene of enzymes participating in biosynthesis of tetrahydrobiopterin and a process for the production of a biopterin compound using the same. In accordance with the present invention, the biopterin compound can be produced in large quantities in an industrial advantageous manner from less expensive materials.Type: GrantFiled: August 2, 2005Date of Patent: October 5, 2010Assignee: Asubio Pharma Co., Ltd.Inventors: Masayuki Yabuta, Kazuaki Furukawa, Nobue Miyamoto, Katsuhiko Yamamoto, Kazuhiro Ohsuye
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Patent number: 7547529Abstract: A method for reducing the formation of a byproduct polypeptide containing an O-acetylserine residue in place of a serine residue by adding at least one of histidine, methionine or glycine to the medium in a method for producing a polypeptide containing a serine residue by culturing transformed cells, and a method for producing a polypeptide containing a serine residue by culturing transformed cells, characterized by reducing the formation of a byproduct polypeptide containing an O-acetylserine residue in place of a serine residue by adding at least one of histidine, methionine or glycine to the medium.Type: GrantFiled: May 10, 2001Date of Patent: June 16, 2009Assignee: Asubio Pharma Co., Ltd.Inventors: Masayuki Yabuta, Toshihiro Sawano, Yumiko Masuda, Kazuhiro Ohsuye
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Patent number: 7344856Abstract: Methods of using OmpT protease are provided. In particular, the invention provides for a method of controlling cleavage of a polypeptide by OmpT protease comprising, converting a sequence site comprising two arbitrary consecutive amino acids and/or amino acid(s) in the vicinity of the site in the polypeptide into other amino acids; which method comprises setting lysine or arginine as the amino acid at the ?1-position concerning the site and setting a specific amino acid as the amino acid at the +1-position; and/or setting specific amino acid(s) as the amino acid(s) at the ?4-position and/or the ?6-position relative to the site; so that a desired part of the polypeptide is cleaved by OmpT protease and/or an undesired part of the polypeptide is not (or hardly) cleaved by OmpT protease. A method of producing a target polypeptide is also provided.Type: GrantFiled: March 3, 2000Date of Patent: March 18, 2008Assignee: Asubio Pharma Co. Ltd.Inventors: Kazuaki Okuno, Masayuki Yabuta, Kazuhiro Ohsuye
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Publication number: 20060008869Abstract: The present invention provides a transformed cell which is transformed by at least one gene of enzymes participating in biosynthesis of tetrahydrobiopterin and a process for the production of a biopterin compound using the same. In accordance with the present invention, the biopterin compound can be produced in large quantities in an industrial advantageous manner from less expensive materials.Type: ApplicationFiled: August 2, 2005Publication date: January 12, 2006Inventors: Masayuki Yabuta, Kazuaki Furukawa, Nobue Miyamoto, Katsuhiko Yamamoto, Kazuhiro Ohsuye
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Publication number: 20040014167Abstract: The present invention provides a transformed cell which is transformed by at least one gene of enzymes participating in biosynthesis of tetrahydrobiopterin and a process for the production of a biopterin compound using the same. In accordance with the present invention, the biopterin compound can be produced in large quantities in an industrial advantageous manner from less expensive materials.Type: ApplicationFiled: April 26, 2003Publication date: January 22, 2004Inventors: Masayuki Yabuta, Kazuaki Furukawa, Nobue Miyamoto, Katsuhiko Yamamoto, Kazuhiro Ohsuye
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Patent number: 6602681Abstract: A C-terminal &agr;-amidating enzyme of Xenopus laevis and precursor thereof produced by a recombinant DNA technique; a DNA coding for the enzyme or precursor thereof; a plasmid containing the DNA; a host organism transformed with the plasmid; a process for production of the enzyme using the transformant; and a process for production of a C-terminal &agr;-amidated peptide using the enzyme.Type: GrantFiled: April 25, 1997Date of Patent: August 5, 2003Assignees: Daiichi Suntory Pharma Co., Ltd.Inventors: Kazuhiro Ohsuye, Katsuhiko Kitano, Shoji Tanaka, Hisayuki Matsuo, Kensaku Mizuno
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Patent number: 6262232Abstract: A C-terminal &agr;-amidating enzyme of Xenopus laevis and precursor thereof produced by a recombinant DNA technique; a DNA coding for the enzyme or precursor thereof; a plasmid containing the DNA; a host organism transformed with the plasmid; a process for production of the enzyme using the transformant; and a process for production of a C-terminal &agr;-amidated peptide using the enzyme.Type: GrantFiled: July 15, 1988Date of Patent: July 17, 2001Assignees: Suntory Limited, Hisayuki MatsuoInventors: Kazuhiro Ohsuye, Katsuhiko Kitano, Shoji Tanaka, Hisayuki Matsuo, Kensaku Mizuno
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Patent number: 6245887Abstract: A C-terminal &agr;-amidating enzyme of Xenopus laevis and precursor thereof produced by a recombinant DNA technique; a DNA coding for the enzyme or precursor thereof; a plasmid containing the DNA; a host organism transformed with the plasmid; a process for production of the enzyme using the transformant; and a process for production of a C-terminal &agr;-amidated peptide using the enzyme.Type: GrantFiled: April 16, 1990Date of Patent: June 12, 2001Assignee: Suntory LimitedInventors: Kazuhiro Ohsuye, Katsuhiko Kitano, Shoji Tanaka, Hisayuki Matsuo, Kensaku Mizuno
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Patent number: 6037145Abstract: A process for the production of a desired polypeptide comprising the steps of: (1) transforming host cells with an expression vector comprising a gene coding for a fusion protein comprising a desired polypeptide and a protective polypeptide; (2) culturing the transformed host cells so as to express said gene to produce a fusion protein; and (3) excising the desired polypeptide from the fusion protein with a protease intrinsic to the host cells. According to the present invention, a large amount of a desired polypeptide can be produced at a low cost. Especially according to the present invention, a large amount of S. aureus V8 protease can be efficiently produced at low cost using a safe host such as E. coli according to gene recombination procedures.Type: GrantFiled: September 5, 1995Date of Patent: March 14, 2000Assignee: Suntory LimitedInventors: Masayuki Yabuta, Kazuhiro Ohsuye
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Patent number: 5976833Abstract: The object of the present invention is to provide a method for improving productivity in the production of useful substances by animal cells. The present invention discloses a method for animal cell culture to produce a desired substance, comprising the steps of (1) culturing animal cells at a temperature at which the animal cells can grow; and (2) culturing the animal cells at a lower temperature.Type: GrantFiled: September 19, 1996Date of Patent: November 2, 1999Assignee: Suntory LimitedInventors: Kazuaki Furukawa, Kazuhiro Ohsuye
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Patent number: 5955307Abstract: Escherichia coli plasmid vectors are provided which have a 5'-terminal untranslated region (inclusive of the promoter region and Shine-Dalgarno sequence) of the Escherichia coli lipoprotein gene, which region is improved to thereby enable direct production of useful polypeptides in substantially complete form.Type: GrantFiled: August 21, 1997Date of Patent: September 21, 1999Assignee: Suntory LimitedInventors: Kazuhiro Ohsuye, Shoji Tanaka
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Patent number: 5834249Abstract: A process for production of a desired protein comprising the steps of:a culturing animal cells capable of producing the desired protein in a medium containing trichostatin compounds; andrecovering the desired protein from the culture.Type: GrantFiled: December 3, 1996Date of Patent: November 10, 1998Assignee: Suntory LimitedInventors: Kazuaki Furukawa, Keijiro Sugimura, Kazuhiro Ohsuye
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Patent number: 5821083Abstract: A C-terminal .alpha.-amidating enzyme of Xenopus laevis and precursor thereof produced by a recombinant DNA technique; a DNA coding for the enzyme or precursor thereof; a plasmid containing the DNA; a host organism transformed with the plasmid; a process for production of the enzyme using the transformant; and a process for production of a C-terminal .alpha.-amidated peptide using the enzyme.Type: GrantFiled: December 4, 1996Date of Patent: October 13, 1998Assignees: Suntory Limited, Hisayuki MatsuoInventors: Kazuhiro Ohsuye, Katsuhiko Kitano, Shoji Tanaka, Hisayuki Matsuo, Kensaku Mizuno
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Patent number: 5747321Abstract: Mutant proteases are obtained with one or more mutation sites in the natural V8 protease protein, and with enzyme activities even in the presence of high urea concentrations.Inactivation of enzyme activity is minimized even in the presence of high concentrations of urea, to thus allow lower amounts of enzyme to be added to urea-containing reaction systems and shorten reaction times. As an additional advantage, the ability to cleave proteins in the presence of high urea concentrations makes it possible to obtain hitherto unobtainable peptide fragments.Type: GrantFiled: June 3, 1996Date of Patent: May 5, 1998Assignee: Suntory LimitedInventors: Masayuki Yabuta, Kazuhiro Ohsuye
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Patent number: 5670340Abstract: The present invention is a process to express a target peptide in a large amount and accumulate the target peptide in host cells in the form of inclusion bodies. The process comprises:A) culturing host cells transformed with a plasmid able to express a gene coding for a fusion protein represented in the formula A--L--B, wherein B is a target peptide, A is a protective peptide comprising a 90-210 amino acid fragment E. coli .beta.-galactosidase, and L is a linker peptide positioned between the C-terminus of the protective peptide and the N-terminus of the target peptide and selected so that when the fusion protein is treated by an enzyme or chemical substance, the target peptide is separated, and wherein the protective peptide and linker peptide are selected so that the isoelectric point of the fusion protein in between 4.9 and 6.Type: GrantFiled: December 5, 1994Date of Patent: September 23, 1997Assignee: Suntory LimitedInventors: Masayuki Yabuta, Yuji Suzuki, Kazuhiro Ohsuye, Takehiro Oshima, Seiko Onai, Koji Magota, Shoji Tanaka
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Patent number: 5622840Abstract: A lactose repressor protein wherein at least one amino acid at the position of 94, 241, 265 or 300 in the wild lactose repressor is replaced with an amino acid other than that of the wild lactose repressor, and the use thereof.The present mutant lactose repressor represses the expression of a desired gene at 30.degree. C. or lower temperature, and induces the expression of a desired gene at 37.degree. C. or higher temperature, and therefor can control the expression by change of a culture temperature without using an expensive inducer such as IPTG.Type: GrantFiled: June 1, 1995Date of Patent: April 22, 1997Assignee: Suntory LimitedInventors: Masayuki Yabuta, Seiko Miura, Kazuhiro Ohsuye
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Patent number: 5252482Abstract: A precursor of a C-terminal amidated peptide represented by the general formula P-X-Gly-Y.sub.n, wherein P is a peptide residue. X is an amino acid residue the C terminal of which (on the Gly side) can be converted in vivo to a --CONH.sub.2 group. Gly is a glycine residue, Y is a basic amino acid residue, n is an interger of 2 to 4 and a further amino acid residue other than Y or a peptide residue may be attached to Y.sub.n, is produced by a gene engineering technology. The precursor exhibits in vivo physiological activity like the C-terminal amidated peptide.Type: GrantFiled: November 5, 1990Date of Patent: October 12, 1993Assignee: Suntory LimitedInventors: Shoji Tanaka, Kazuhiro Ohsuye, Ichiro Kubota, Norio Ohnuma, Teruhisa Noguchi