Abstract: Disclosed is a method for producing 1-(2-hydroxyethyl)-2,5,5,8a-tetramethyldecahydronaphthalene-2-ol represented by formula (2), wherein microbial conversion is carried out using a compound(s) represented by formula (1a) and/or (1b) as a substrate, the resulting culture product, in which microorganisms obtained by the microbial conversion are contained, and a solvent having an SP value within the range of 7.5 to 9.0 [(cal/cm3)1/2] are mixed together, and subsequently the aqueous phase is removed therefrom.

Abstract: A method for producing an immobilized enzyme which includes the steps of immobilizing an enzyme used for hydrolyzing fats and oils on a immobilization carrier by adsorption, bringing the immobilized enzyme into contact with an organic solvent in which fat-soluble fatty acids or the derivatives thereof have been dissolved, and adjusting the moisture content of the immobilized enzyme from 1 to 20% by weight based on the weight of the carrier.

Abstract: The present invention relates to a method for production of a diol form represented by the formula (2), which includes filtering a culture fluid obtained by microbial conversion using a compound represented by the following formula (1a) and/or formula (1b) as a substrate, using a filter having an aperture size of 10 to 100 ?m; washing the residue on the filter with water or a solvent having an SP value outside the range of 8.3 to 20 (cal/cm3)1/2; subsequently dissolving the obtained cake in a solvent having an SP value of 8.3 to 20 (cal/cm3)1/2; and then filtering or centrifuging the solution. According to the present invention, 1-(2-hydroxyethyl)-2,5,5,8a-tetramethyldecahydronaphthalen-2-ol, which is useful as an intermediate for the production of 3a,6,6,9a-tetramethyldodecahydronaphtho[2,1-b]furan, can be efficiently produced at high purity.

Abstract: Disclosed is a method for producing 1-(2-hydroxyethyl) 2,5,5,8a-tetramethyldecahydronaphthalene-2-ol represented by formula (2), wherein microbial conversion is carried out using a compound(s) represented by formula (1a) and/or (1b) as a substrate, the resulting culture product, in which microorganisms obtained by the microbial conversion are contained, and a solvent having an SP value within the range of 7.5 to 9.0 [(cal/cm3)1/2] are mixed together, and subsequently the aqueous phase is removed therefrom.

Abstract: Provided is a process for producing a diol, which can efficiently produce a diol having a low intensity of abnormal odor and high purity. A process for producing 1-(2-hydroxyethyl)-2,5,5,8a-tetramethyldecahydronaphthalen-2-ol represented by formula (2): the process containing drying a culture fluid obtained by microbial conversion using a compound(s) represented by formula (1a) and/or (1b) as a substrate, bringing the dried product of the culture fluid into contact with such a solvent that the SP value of the medium obtainable after the contact between the dried product of the culture fluid and the solvent falls in the range of 9.5 to 16 [(cal/cm3)1/2], subsequently removing the microorganism from the medium, and crystallizing the medium.

Abstract: A method for producing an immobilized enzyme which includes the steps of immobilizing an enzyme used for hydrolyzing fats and oils on a immobilization carrier by adsorption, bringing the immobilized enzyme into contact with an organic solvent in which fat-soluble fatty acids or the derivatives thereof have been dissolved, and adjusting the moisture content of the immobilized enzyme from 1 to 20% by weight based on the weight of the carrier.

Abstract: A method for producing an immobilized enzyme which includes the steps of immobilizing an enzyme used for hydrolyzing fats and oils on a immobilization carrier by adsorption, bringing the immobilized enzyme into contact with an organic solvent in which fat-soluble fatty acids or the derivatives thereof have been dissolved, and adjusting the moisture content of the immobilized enzyme from 1 to 20% by weight based on the weight of the carrier.

Abstract: The present invention relates to a method for production of a diol form represented by the formula (2), which includes filtering a culture fluid obtained by microbial conversion using a compound represented by the following formula (1a) and/or formula (1b) as a substrate, using a filter having an aperture size of 10 to 100 ?m; washing the residue on the filter with water or a solvent having an SP value outside the range of 8.3 to 20 (cal/cm3)1/2; subsequently dissolving the obtained cake in a solvent having an SP value of 8.3 to 20 (cal/cm3)1/2; and then filtering or centrifuging the solution. According to the present invention, 1-(2-hydroxyethyl)-2,5,5,8a-tetramethyldecahydronaphthalen-2-ol, which is useful as an intermediate for the production of 3a,6,6,9a-tetramethyldodecahydronaphtho[2,1-b]furan, can be efficiently produced at high purity.

Abstract: Provided is a preparation process of diglycerides which comprises reacting a fatty acid or lower alkyl ester thereof with glycerin in the presence of an immobilized partial glyceride lipase while removing water, which is generated upon reaction, out of the system. The reaction is preferably terminated when the acid value (AV) of the reaction mixture satisfies the following range: 50R-60>AV>70R-150 (with the proviso that AV>0). The present process makes it possible to efficiently prepare a high purity of diglycerides without impairing lipase activity even under reduced pressure. The present process is effective without being influenced by variations in reaction conditions such as fatty acid/glycerin ratio, reaction temperature or enzyme concentration. Particularly, even when the fatty acid/glycerin ratio is high, a high purity of diglycerides can be produced at a high reaction yield.

Abstract: A method for producing an immobilized enzyme which includes the steps of immobilizing an enzyme used for hydrolyzing fats and oils on a immobilization carrier by adsorption, bringing the immobilized enzyme into contact with an organic solvent in which fat-soluble fatty acids or the derivatives thereof have been dissolved, and adjusting the moisture content of the immobilized enzyme from 1 to 20% by weight based on the weight of the carrier.

Abstract: Provided is a preparation process of diglycerides which comprises reacting a fatty acid or lower alkyl ester thereof with glycerin in the presence of an immobilized partial glyceride lipase while removing water, which is generated upon reaction, out of the system. The reaction is preferably terminated when the acid value (AV) of the reaction mixture satisfies the following range: 50R-60>AV>70R-150 (with the proviso that AV>0). The present process makes it possible to efficiently prepare a high purity of diglycerides without impairing lipase activity even under reduced pressure. The present process is effective without being influenced by variations in reaction conditions such as fatty acid/glycerin ratio, reaction temperature or enzyme concentration. Particularly, even when the fatty acid/glycerin ratio is high, a high purity of diglycerides can be produced at a high reaction yield.