Abstract: The present invention provides a method for amplifying, in a reaction solution, at least one target gene having a GC content of at least 60%, wherein the method contains a step of amplifying a specific base sequence in a target gene by using a primer set consisting of a first primer and a second primer for target gene amplification, and wherein one primer in the primer set is added to the reaction solution at a molar ratio higher than the other primer and neither primer is a hot start primer that contains an inactivating chemical modification that is restored by an action of an activating enzyme.

Abstract: Disclosed is a method for identifying causative bacteria of sepsis, the method comprising: a step of performing a PCR method using a sample collected from a subject and a combination of sets of primers each specific to the full-length or a partial region of each of gyrB gene of Escherichia coli, nuc gene of Staphylococcus aureus, atlE gene of Staphylococcus epidermidis, gyrB gene of Klebsiella pneumoniae and rpoB gene of Enterococcus spp.; and a step of detecting the presence or absence of amplification of the full-length or the partial region of each of the genes by using a combination of probes each specific to DNA of the full-length or the partial region.

Abstract: The present invention provides a primer set for detecting the presence of Staphylococcus argenteus in a specimen, the primer set comprising: a first primer; and a second primer, in which the first primer and the second primer target a nuc gene of Staphylococcus argenteus.

Abstract: The present invention provides a primer set for detecting the presence of Klebsiella variicola in a specimen, wherein the primer set consists of a first primer and a second primer that target the gyrB gene of Klebsiella variicola.

Abstract: Provided is an antibacterial drug for an enterobacterium that causes an infection in humans, such as Clostridioides difficile. An antibacterial agent against an enterobacterium, comprising equol as an active ingredient.