Abstract: As a technique for detecting an influenza virus with an improved accuracy, there is provided a method for detecting an influenza virus in a biological sample by using a first probe, which is decomposed by an influenza virus-derived neuraminidase and a bacterium-derived neuraminidase to generate an optically detectable signal, and a second probe, which is decomposed by the bacterium-derived neuraminidase to generate an optically detectable signal and not decomposed by the influenza virus-derived neuraminidase.

Abstract: Provided is a technique that can be used to detect a pathogenic microorganism such as influenza virus with high sensitivity, and specifically, a method for detecting a reaction product, including reacting an enzyme with a substance serving as a substrate for a reaction involving the enzyme in a hydrophilic solvent that is in interfacial contact with a hydrophobic solvent, wherein the reaction product is produced directly and released from the substrate, and wherein the hydrophilic solvent contains at least one of a buffering substance or trimethylamine oxide (TMAO).

Abstract: Provided is a digital assay method for determining the concentration of a substance in a solution, as a method available for making the operation of digital assay simpler and easier, the method comprising the step of: covering a chamber array region of a substrate with an adhesive tape comprising a base layer and an adhesive layer, followed by pressure-bonding the adhesive layer of the adhesive tape against the surface of the substrate, thereby discharging excess of a solution in the chamber array region outside the chamber array region and sealing each of the chambers containing the solution liquid-tight to function as reactors.

Abstract: As a technique for detecting an influenza virus with an improved accuracy, there is provided a method for detecting an influenza virus in a biological sample by using a first probe, which is decomposed by an influenza virus-derived neuraminidase and a bacterium-derived neuraminidase to generate an optically detectable signal, and a second probe, which is decomposed by the bacterium-derived neuraminidase to generate an optically detectable signal and not decomposed by the influenza virus-derived neuraminidase.

Abstract: Provided is a method of enclosing a microscopic body in at least some of a plurality of cavities formed in the surface of a substrate, including the step of arranging an insertion member above the cavity-formed surface of the substrate, determining relative positions of the insertion member and the substrate by a support section provided on the insertion member such that the bottom surface of the insertion member and the cavity-formed surface of the substrate face each other, thereby providing a solution introduction space between the bottom surface of the insertion member and the cavity-formed surface of the substrate, and providing a solution discharge space that is in communication with the solution introduction space, the solution discharge space being located above the bottom surface of the insertion member, and between the substrate and the insertion member, within the substrate and/or within the insertion member.

Abstract: Provided is a method for detecting pathogenic microorganisms in a biological sample, which is a technique that can be used to perform high-sensitivity detection of pathogenic microorganisms, such as influenza virus, etc.

Abstract: Provided is a technique that can be used to detect a pathogenic microorganism such as influenza virus with high sensitivity, and specifically, a method for detecting a reaction product, including reacting an enzyme with a substance serving as a substrate for a reaction involving the enzyme in a hydrophilic solvent that is in interfacial contact with a hydrophobic solvent, wherein the reaction product is produced directly and released from the substrate, and wherein the hydrophilic solvent contains at least one of a buffering substance or trimethylamine oxide (TMAO).

Abstract: Provided is a method of enclosing a microscopic body in at least some of a plurality of cavities formed in the surface of a substrate, including the step of arranging an insertion member above the cavity-formed surface of the substrate, determining relative positions of the insertion member and the substrate by a support section provided on the insertion member such that the bottom surface of the insertion member and the cavity-formed surface of the substrate face each other, thereby providing a solution introduction space between the bottom surface of the insertion member and the cavity-formed surface of the substrate, and providing a solution discharge space that is in communication with the solution introduction space, the solution discharge space being located above the bottom surface of the insertion member, and between the substrate and the insertion member, within the substrate and/or within the insertion member.

Abstract: Provided is a method for detecting pathogenic microorganisms in a biological sample, which is a technique that can be used to perform high-sensitivity detection of pathogenic microorganisms, such as influenza virus, etc.

Abstract: Disclosed is a substrate (10A) for trapping a microorganism or cell (T), characterized by comprising a base (4) and having a space (2) into which a fluid (R) containing the microorganism or cell (T) is introduced and a microfine suction hole (1) through which the space (2) communicates with the outside of the base (4). The substrate is further characterized in that the space (2) has been formed in the base (4), and at least the portion of the base (4) which forms the microfine suction hole (1) is constituted of a single member.

Abstract: A method of manufacturing a base body having a microscopic hole, includes: forming at least one of a first modified region and a second modified region by scanning inside of a base body with a focal point of a first laser light having a pulse duration on order of picoseconds or less; forming a periodic modified group formed of a plurality of third modified regions and fourth modified regions by scanning an inside of the base body with a focal point of a second laser light having a pulse duration on order of picoseconds or less; obtaining the base body which is formed so that the first modified region and the second modified region overlap or come into contact with the modified group; and forming a microscopic hole by removing the first modified region and the third modified regions by etching.

Abstract: A method of manufacturing a base body having a microscopic hole, includes: forming at least one of a first modified region and a second modified region by scanning inside of a base body with a focal point of a first laser light having a pulse duration on order of picoseconds or less; forming a periodic modified group formed of a plurality of third modified regions and fourth modified regions by scanning an inside of the base body with a focal point of a second laser light having a pulse duration on order of picoseconds or less; obtaining the base body which is formed so that the first modified region and the second modified region overlap or come into contact with the modified group; and forming a microscopic hole by removing the first modified region and the third modified regions by etching.

Abstract: A base body includes: a base member; a channel provided in the base member, the channel having an inner wall surface and flowing a fluid; a fine vacuum hole provided in the inner wall, the fine vacuum hole causing the channel to communicate the outside of the base member other and having an opening; and a slow flow portion disposed at a position close to the opening of the fine vacuum hole in the inner wall surface, the slow flow portion slows a flow of the fluid, wherein at least a portion that configures the fine vacuum hole in the base member is formed of a single member.